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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||224 Antibodies||98 Antibodies||95 Antibodies|
|Immunoprecipitation (IP)||23 Antibodies||5 Antibodies||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||53 Antibodies||29 Antibodies||29 Antibodies|
|Immunohistochemistry (IHC)||122 Antibodies||68 Antibodies||67 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||44 Antibodies||44 Antibodies||44 Antibodies|
|Immunofluorescence (IF)||57 Antibodies||36 Antibodies||36 Antibodies|
|Antigen||P21-Activated Kinase 1 (PAK1) Antibodies|
|Epitope||pThr402, pThr423 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PAK1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Product Details anti-PAK1 AntibodyTarget Details PAK1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of p-PAK1/2 protein, only when phosphorylated at Thr423/402.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details PAK1Product Details anti-PAK1 Antibody Application Details Handling Images back to top
|Alternative Name||PAK1 (PAK1 Antibody Abstract)|
|Background||Three isoforms of Serine/Threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac 1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. The site of autophosphorylation on αPAK is Thr 423, which is in the kinase activation loop. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK-4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac 1 and Cdc42, they do not interact with Rho.Synonyms: Alpha-PAK, Gamma-PAK, PAK 1, PAK 2, PAK alpha, PAK gamma, PAK-1, PAK-2, PAK65, Serine/threonine-protein kinase PAK 1, Serine/threonine-protein kinase PAK 2, p21-activated kinase 1, p21-activated kinase 2, p58, p65-PAK|
|Molecular Weight||approx. 60 kDa|
|Research Area||Cancer, Apoptosis/Necrosis, Kinases/Phosphatases|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-PAK1 Antibody Target Details PAK1 Handling Images back to top
Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PAK1 Antibody Target Details PAK1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-PAK1 Antibody Target Details PAK1 Application Details Handling back to top