|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||33 Antibodies||33 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||1 Antibodies|
|Immunochromatography (IC)||3 Antibodies||3 Antibodies|
|Immunocytochemistry (ICC)||11 Antibodies||11 Antibodies|
|Immunofluorescence (IF)||36 Antibodies||36 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||44 Antibodies||44 Antibodies|
|Immunohistochemistry (IHC)||67 Antibodies||68 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||29 Antibodies||29 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Western Blotting (WB)||95 Antibodies|
|Antigen||P21-Activated Kinase 1 (PAK1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PAK1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-PAK1 AntibodyTarget Details PAK1 Application Details Handling Images
|Purification||Tissue culture supernatant|
|Immunogen||A synthetic peptide corresponding to residues in the N-terminus of human PAK1 protein was used as immunogen.|
Target Details PAK1Product Details anti-PAK1 Antibody Application Details Handling Images back to top
|Alternative Name||PAK1 (PAK1 Antibody Abstract)|
P21-activated kinase 1 (PAK1) is a member of a family of serine/threonine proteinkinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). ThePAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 andPAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). PAK1participates in regulating the actin cytoskeleton, focal adhesion contacts, cell motility,apoptosis and transcription. It also promotes the disassembly of stress fibers (4-5).PAK1 also binds PIX (PAK-binding protein), COOL and adapter protein Nck (5). Alternate Names: anti-Alpha PAK antibody, anti-Alpha PAK antibody, anti-p21 activated kinase 1 antibody,anti-p21 activated kinase1 antibody, anti-p21/Cdc42/Rac1 activated kinase 1 antibody,anti-p65 PAK antibody, anti-PAK 1 antibody, anti-PAK alpha antibody,anti-Serine/threonine protein kinase PAK 1 antibody, anti-Serine/threonine proteinkinase PAK1 antibody, anti-STE20 homolog (yeast) antibody.
Gene Symbol: PAK1
|Gene ID||5058, 18479, 29431|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-PAK1 Antibody Target Details PAK1 Handling Images back to top
|Application Notes||Recommended dilutions: Flow Cytometry 1:10, Immunocytochemistry/Immunofluorescence 1:50-100, Immunohistochemistry 1:100-250, Immunohistochemistry-Paraffin 1:100-250, Immunoprecipitation 1:50, Western Blot 1:500|
Protocol specific for PAK1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-PAK1 Antibody Target Details PAK1 Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
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