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anti-Cat (Feline) SIRT1 antibody for Western Blotting

Recommended SIRT1 Antibody (supplied by: Log in to see )

Antigen
Sirtuin 1 (SIRT1) Antibodies
  • AA673258
  • SIR2
  • Sir2
  • Sir2a
  • Sir2alpha
  • SIR2L1
  • sir2l1
  • sirtuin
  • sirtuin 1
Epitope
AA 33-55
30
21
20
17
17
16
13
13
10
5
4
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Chicken, Cat (Feline), Human, Mouse (Murine), Rat (Rattus)
252
103
45
8
8
5
4
4
2
2
2
2
2
2
1
1
Host
Rabbit
182
57
25
7
2
Clonality
Polyclonal
Conjugate
This SIRT1 antibody is un-conjugated
14
12
9
5
5
4
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
Application
Western Blotting (WB)
212
112
57
42
41
34
28
25
11
5
4
4
4
2
1
1
Supplier
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Catalog No. ABIN493460
$ 456.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011728023 ABIN4353904 SimWes WB Rabbit Log in to see Polyclonal
0.0011728023 ABIN4353905 SimWes WB Rabbit Log in to see Polyclonal

Top referenced anti-SIRT1 antibody for Western Blotting

Similar anti-SIRT1 Antibodies

Application / Reactivity Human Chicken Mouse (Murine) Rat (Rattus) Cow (Bovine) Cat (Feline)
Western Blotting (WB) 204 Antibodies 6 Antibodies 68 Antibodies 33 Antibodies 3 Antibodies 3 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 29 Antibodies 2 Antibodies 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 40 Antibodies 14 Antibodies 6 Antibodies
Immunohistochemistry (IHC) 58 Antibodies 15 Antibodies

General

Antigen Sirtuin 1 (SIRT1) Antibodies
Epitope AA 33-55
(30), (21), (20), (17), (17), (16), (13), (13), (10), (5), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Chicken, Cat (Feline), Human, Mouse (Murine), Rat (Rattus)
(252), (103), (45), (8), (8), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(182), (57), (25), (7), (2)
Clonality
Polyclonal
Conjugate This SIRT1 antibody is un-conjugated
(14), (12), (9), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(212), (112), (57), (42), (41), (34), (28), (25), (11), (5), (4), (4), (4), (2), (1), (1)
Pubmed 4 references available
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Product Details anti-SIRT1 Antibody

Target Details SIRT1 Application Details Handling References for anti-SIRT1 Antibody (ABIN493460) Images
Specificity This antibody label a 90-92 kDa and some smaller bands (identity of these bands is not known) in Western blot positive control samples.
Cross-Reactivity (Details) Species reactivity (expected):Bovine, Zebrafish, Chicken and CatSpecies reactivity (tested):Human, Mouse and Rat.
Purification Affinity Chromatography on immobilized antigen
Immunogen Synthetic peptide corresponding to amino acids 33-55 of Rat Sirtuin-1.
Isotype IgG

Target Details SIRT1

Product Details anti-SIRT1 Antibody Application Details Handling References for anti-SIRT1 Antibody (ABIN493460) Images back to top
Antigen
Alternative Name SIRT1 (SIRT1 Antibody Abstract)
Background Silent information regulators (Sir proteins) or atypical class III HDACs (NAD-dependent deacetylase proteins) is a member of the Sir2 family of NAD+-dependent protein deacetylases involved in caloric restriction-dependent life span extension in diverse organisms but their role in humans have not been elucidated fully. The cell survival is partly controlled by activity of NFkB and Sir 1 controls the transcriptional activity of NFkB. Sir1 is a mammalian homolog of yeast SIR2 (silencing Information Regulator) and is a member of a larger family of Sirtuin proteins (Sir1 thru Sir7) which are implicated in modulating transacriptional silencing and cell survival. Sir 1 interacts with RelA/p65 subunit of NF-kB and inhibits transcription by de-acetylating RelA/p65 at lysine 310 (1). SIRT1 plays an active role in inhibiting transcription by interacting with the basic helix-loop-helix proteins HES1, HEY2, and with the COUP-TF interacting protein 2 (CTIP2, 2). Sir1 also deactylates non-histone protein substrate, including TAFI68, PCAF, p300, MyoD, p53, and Ku 70. SIRT1 regulates cell fate, in part, by deacetylating the p53 protein at lysine 382 and inactivating p53-mediated transcription and apoptosis. Recently, SIRT1 was shown to control Bax-induced apoptosis by de-acetylating Ku70, and to inhibit Forkhead-mediated cell death (3). While SIRT1 is capable of protecting cells from p53-induced apoptosis, our work provides evidence that SIRT1 activity augments apoptosis in response to TNFα by the ability of the deacetylase to inhibit the transactivation potential of the RelA/p65 protein. Evidence suggests that histone deacetylase, SIRT1, is a mediator of life span extension by calorie restriction, however, in some cases the HDAC activity is necessary for the maintenance and survival and growth advantage in some types of cancer. SIRT1 may paradoxically increase the risk of cancer and acts as tumor promoter, it should be considered as a therapeutic target for the treatment of cancers (4). Sir1 protein has 737 amino acids, that is significantly elevated in human and mouse prostate cancer (5). SIRT1 represses Forkhead transcriptional factor (FOX3) and also acts a corepressor of androgen receptor and suggest a new molecular pathway relevant to prostate cancer growth and approaches to therapy.Synonyms: NAD-dependent deacetylase sirtuin-1, S. cerevisiae, SIR2-like protein 1, SIR2L1, SIR2alpha, SIRT-1, homolog) 1, sirtuin (silent mating type information regulation 2, sirtuin 1, sirtuin type 1, sirtuin-1
Gene ID 23411
NCBI Accession NP_036370
UniProt Q96EB6
Research Area Chromatin and Nuclear Signaling, Apoptosis/Necrosis, Proteases
Pathways MAPK Signaling

Application Details

Product Details anti-SIRT1 Antibody Target Details SIRT1 Handling References for anti-SIRT1 Antibody (ABIN493460) Images back to top
Application Notes This antibody is ideal for Western Blot on Rat, Human and Mouse samples. Recommended Dilution: Western Blot: > 1/500
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details References for anti-SIRT1 Antibody (ABIN493460) Images back to top
Concentration 0.69-0.75 mg/mL
Buffer Stabilization buffer
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-SIRT1 Antibody (ABIN493460)

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling Images back to top
Background publications

Huffman, Grizzle, Bamman, Kim, Eltoum, Elgavish, Nagy: "SIRT1 is significantly elevated in mouse and human prostate cancer." in: Cancer research, Vol. 67, Issue 14, pp. 6612-8, 2007

Yeung, Hoberg, Ramsey, Keller, Jones, Frye, Mayo: "Modulation of NF-kappaB-dependent transcription and cell survival by the SIRT1 deacetylase." in: The EMBO journal, Vol. 23, Issue 12, pp. 2369-80, 2004

Motta, Divecha, Lemieux, Kamel, Chen, Gu, Bultsma, McBurney, Guarente: "Mammalian SIRT1 represses forkhead transcription factors." in: Cell, Vol. 116, Issue 4, pp. 551-63, 2004

Cohen, Lavu, Bitterman, Hekking, Imahiyerobo, Miller, Frye, Ploegh, Kessler, Sinclair: "Acetylation of the C terminus of Ku70 by CBP and PCAF controls Bax-mediated apoptosis." in: Molecular cell, Vol. 13, Issue 5, pp. 627-38, 2004