You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Mouse (Murine) SIRT1 antibody for Immunocytochemistry

Recommended SIRT1 Antibody (supplied by: Log in to see )

Antigen
Sirtuin 1 (SIRT1) Antibodies
  • AA673258
  • SIR2
  • Sir2
  • Sir2a
  • Sir2alpha
  • SIR2L1
  • sir2l1
  • sirtuin
  • sirtuin 1
Epitope
AA 236-490
21
20
19
17
16
13
13
10
5
4
4
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
217
98
43
8
6
4
2
1
1
1
1
Host
Rabbit
150
54
25
6
2
Clonality
Polyclonal
Conjugate
This SIRT1 antibody is un-conjugated
12
11
7
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
176
108
43
34
32
31
25
17
9
4
4
4
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN1078528
$ 396.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010160999 ABIN1863146 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal

Similar anti-SIRT1 Antibodies

Application / Reactivity Mouse (Murine)
ELISA 38 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 13 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 63 Antibodies

General

Antigen Sirtuin 1 (SIRT1) Antibodies
Epitope AA 236-490
(21), (20), (19), (17), (16), (13), (13), (10), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(217), (98), (43), (8), (6), (4), (2), (1), (1), (1), (1)
Host Rabbit
(150), (54), (25), (6), (2)
Clonality
Polyclonal
Conjugate This SIRT1 antibody is un-conjugated
(12), (11), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(176), (108), (43), (34), (32), (31), (25), (17), (9), (4), (4), (4), (2), (1), (1)
Supplier Log in to see

Product Details anti-SIRT1 Antibody

Target Details SIRT1 Application Details Handling Images
Sequence MGHHHHHHSG SEF-EDAVK LLQECKKIIV LTGAGVSVSC GIPDFRSRDG IYARLAVDFP DLPDPQAMFD IEYFRKDPRP FFKFAKEIYP GQFQPSLCHK FIALSDKEGK LLRNYTQNID TLEQVAGIQR ILQCHGSFAT ASCLICKYKV DCEAVRGDIF NQVVPRCPRC PADEPLAIMK PEIVFFGENL PEQFHRAMKY DKDEVDLLIV IGSSLKVRPV ALIPSSIPHE VPQILINREP LPHLHFDVEL LGDCDVIINE LCHRLGGEYA
Specificity It has been selected for its ability to recognize SIRT1 in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen Recombinant SIRT1 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against SIRT1.
Isotype IgG

Target Details SIRT1

Product Details anti-SIRT1 Antibody Application Details Handling Images back to top
Antigen
Pathways MAPK Signaling

Application Details

Product Details anti-SIRT1 Antibody Target Details SIRT1 Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant SIRT1 (Glu236~Ala490) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-SIRT1 antibody (Sirtuin 1) (AA 236-490) (ABIN1078528) anti-Sirtuin 1 (SIRT1) (AA 236-490) antibody