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anti-Mouse (Murine) SIRT1 antibody for Immunohistochemistry

Recommended SIRT1 Antibody (supplied by: Log in to see )

Antigen
Sirtuin 1 (SIRT1) Antibodies
  • sirtuin 1
  • AA673258
  • SIR2L1
  • Sir2
  • Sir2a
  • Sir2alpha
  • sir2l1
  • sirtuin
  • SIR2
  • sirtuin 1
  • SIRT1
  • SRT1
  • LOC100349271
  • Sirt1
  • sirt1
Epitope
AA 566-601
29
23
20
18
16
15
15
15
10
5
5
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
288
139
72
21
14
11
7
7
6
5
5
3
3
2
2
2
2
1
1
Host
Rabbit
192
81
27
7
3
Clonality
Polyclonal
Conjugate
This SIRT1 antibody is un-conjugated
15
13
10
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
251
132
82
61
59
48
40
31
30
5
5
4
4
2
2
2
1
Supplier
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Catalog No. ABIN3032583
$ 411.13
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4353901 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 1F3 1
1 ABIN1450758 IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal 3
1 ABIN3186962 ELISA IHC WB Rabbit IgG Tyr631 Log in to see Polyclonal
1 ABIN1525672 IHC WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN3020731 IHC WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1874783 IHC IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN1078528 ICC IHC IP WB Rabbit IgG AA 236-490 Log in to see Polyclonal
1 ABIN1457379 IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN2559933 IHC ELISA WB Goat IgG AA 577-590 Log in to see Polyclonal
1 ABIN2505459 IHC ELISA WB Rabbit IgG Ser47 Log in to see Polyclonal
1 ABIN4353900 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 1F3
1 ABIN2885319 IHC ELISA WB Rabbit IgG AA 41-90 Log in to see Polyclonal
1 ABIN4963891 ELISA FACS ICC IF IHC IHC (p) WB DyLight 350 Mouse IgG1 Log in to see 1F3
1 ABIN4963892 ELISA FACS ICC IF IHC IHC (p) WB DyLight 405 Mouse IgG1 Log in to see 1F3
1 ABIN4963876 ELISA FACS ICC IF IHC IHC (p) WB Alexa Fluor 405 Mouse IgG1 Log in to see 1F3
1 ABIN4963887 ELISA FACS ICC IF IHC IHC (p) WB DyLight 755 Mouse IgG1 Log in to see 1F3
1 ABIN4963884 ELISA FACS ICC IF IHC IHC (p) WB DyLight 680 Mouse IgG1 Log in to see 1F3
1 ABIN4963885 ELISA FACS ICC IF IHC IHC (p) WB DyLight 488 Mouse IgG1 Log in to see 1F3
1 ABIN4963883 ELISA FACS ICC IF IHC IHC (p) FITC Mouse IgG1 Log in to see 1F3
1 ABIN4963890 ELISA FACS ICC IF IHC IHC (p) WB DyLight 550 Mouse IgG1 Log in to see 1F3

Top referenced anti-SIRT1 antibody for Immunohistochemistry

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Enzyme Immunoassay (EIA) 2 Antibodies 4 Antibodies
Flow Cytometry (FACS) 17 Antibodies 30 Antibodies
Immunocytochemistry (ICC) 19 Antibodies 45 Antibodies
Immunofluorescence (IF) 26 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 39 Antibodies
Immunohistochemistry (IHC) 37 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 Antibodies
Immunoprecipitation (IP) 3 Antibodies
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Western Blotting (WB) 95 Antibodies
Chromatin Immunoprecipitation (ChIP) 5 Antibodies
ELISA (Capture) 1 Antibodies
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General

Antigen Sirtuin 1 (SIRT1) Antibodies
Epitope AA 566-601
(29), (23), (20), (18), (16), (15), (15), (15), (10), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(288), (139), (72), (21), (14), (11), (7), (7), (6), (5), (5), (3), (3), (2), (2), (2), (2), (1), (1)
Host Rabbit
(192), (81), (27), (7), (3)
Clonality
Polyclonal
Conjugate This SIRT1 antibody is un-conjugated
(15), (13), (10), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(251), (132), (82), (61), (59), (48), (40), (31), (30), (5), (5), (4), (4), (2), (2), (2), (1)
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Product Details anti-SIRT1 Antibody

Target Details SIRT1 Application Details Handling Images
Purification Antigen affinity purified
Immunogen A portion of amino acids 566-601 of the mouse protein was used as the immunogen for the SIRT1 antibody.
Isotype Ig Fraction

Target Details SIRT1

Product Details anti-SIRT1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name SIRT1 (SIRT1 Antibody Abstract)
Background SIRT1 is a NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. [UniProt]
UniProt Q923E4
Research Area Chromatin and Nuclear Signaling, Apoptosis/Necrosis, Proteases
Pathways MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis

Application Details

Product Details anti-SIRT1 Antibody Target Details SIRT1 Handling Images back to top
Application Notes The stated application concentrations are suggested starting points. Titration of the SIRT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. IHC (Paraffin): 1:25,Immunofluorescence: 1:25,Western blot: 1:1000
Restrictions For Research Use only

Handling

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Images back to top
Format Liquid
Buffer In 1X PBS, pH 7.4, with 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at 4oC for up to one month. For long term, aliquot the SIRT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-SIRT1 antibody (Sirtuin 1) (AA 566-601) (ABIN3032583) Fluorescent image of A549 cells stained with SIRT1 antibody. Ab was diluted at 1:25 d...
Immunohistochemistry (IHC) image for anti-SIRT1 antibody (Sirtuin 1) (AA 566-601) (ABIN3032583) IHC analysis of FFPE mouse brain section using SIRT1 antibody at 1:25.
Western Blotting (WB) image for anti-SIRT1 antibody (Sirtuin 1) (AA 566-601) (ABIN3032583) Western blot analysis of lysate from mouse F9 cell line using SIRT1 antibody at 1:100...