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Cat (Feline) IL1B ELISA Kit

Recommended IL1B Product (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) ELISA Kits
  • IL-1
  • IL-1B
  • Il-1b
  • IL-1beta
  • IL-1BETA
  • il1-b
  • IL1-BETA
  • IL1B
  • IL1beta
  • IL1F2
  • zgc:111873
Reactivity
Cat (Feline)
Alternatives
Kits with alternative reactivity to:
67
39
32
12
9
8
7
6
6
6
5
4
2
2
2
2
1
Methode Type
Sandwich ELISA
Detection Range
16-4.000 pg/mL
Minimum Detection Limit
16 pg/mL
Application
ELISA
Supplier
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Available images

Catalog No. ABIN2703137
$ 666.60
Plus shipping costs $45.00

Similar IL1B ELISA Kits

Application / Reactivity Cat (Feline)
ELISA 1 ELISA Kits

General

Antigen Interleukin 1, beta (IL1B) ELISA Kits
Reactivity Cat (Feline)
Kits with alternative reactivity to:
(67), (39), (32), (12), (9), (8), (7), (6), (6), (6), (5), (4), (2), (2), (2), (2), (1)
Methode Type Sandwich ELISA
Detection Range 16-4.000 pg/mL
Minimum Detection Limit 16 pg/mL
Application ELISA
Supplier Log in to see

Product Details IL1B ELISA Kit

Target details Application Details Handling Images
Purpose Feline IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Cell Culture Supernatant, Plasma, Serum
Analytical Method Quantitative
Specificity This ELISA antibody pair detects feline IL-1 beta. Other species not determined.
Sensitivity 16 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details IL1B ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name IL-1 beta (IL1B ELISA Kit Abstract)
Background Gene Names: IL1B
Protein names: Interleukin-1 beta (IL-1 beta)
Gene ID 768274
UniProt P41687
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details IL1B ELISA Kit Target details Handling Images back to top
Application Notes Recommended Dilution for serum and plasma samples2 fold
Plate Pre-coated,Strips (12 x 8)
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details IL1B ELISA Kit Target details Application Details Images back to top
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

Images

Product Details IL1B ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for IL1B ELISA Kit (Interleukin 1, beta) (ABIN2703137) Interleukin 1, beta (IL1B) ELISA Kit