anti-Human Insulin antibody for Immunocytochemistry

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • insulin-like
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • Ins2
  • LOC100060077
Epitope
AA 25-110
27
20
15
14
12
10
7
6
6
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
949
397
283
274
262
121
49
6
6
5
5
5
4
2
2
1
1
1
1
1
1
1
Host
Mouse
835
182
54
16
3
1
Clonality
Monoclonal
Conjugate
This Insulin antibody is un-conjugated
56
42
41
27
26
26
21
21
18
17
17
17
17
17
17
16
16
12
11
10
9
9
9
5
5
5
5
5
5
2
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
622
372
247
233
205
203
161
137
70
66
40
28
15
11
9
8
8
7
5
4
3
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN4899865
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
26.040287 ABIN4900790 CyTOF FACS ICC Rat IgG2a Log in to see 182410 5
26.040287 ABIN4899864 FACS ICC Biotin Mouse IgG2a AA 25-110 Log in to see 253627
1 ABIN617877 IEM ICC IF IHC Rabbit IgG Log in to see Polyclonal 44
1 ABIN269653 DB ICC IF IHC (p) IHC RIA Mouse IgG1 Log in to see K36aC10
1 ABIN337249 FACS ICC IF IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN4326102 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see SPM139
1 ABIN269045 ICC IF IHC (p) IHC RIA ELISA Mouse IgG1 Log in to see C-PEP-01
1 ABIN94408 Func ICC IHC (p) RIA ELISA Mouse IgG1 Log in to see IN-05 1
1 ABIN4326053 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326054 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326055 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326056 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326057 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326058 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN206973 BR ELISA Func ICC IHC (p) Neut RIA Mouse IgG1 Log in to see IN-05
1 ABIN4904068 ICC IF IHC Rabbit IgG Log in to see
1 ABIN1735946 ICC FACS IF IHC (p) ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN4326067 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326068 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326069 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)

General

Antigen Insulin (INS) Antibodies
Epitope AA 25-110
(27), (20), (15), (14), (12), (10), (7), (6), (6), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(949), (397), (283), (274), (262), (121), (49), (6), (6), (5), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(835), (182), (54), (16), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is un-conjugated
(56), (42), (41), (27), (26), (26), (21), (21), (18), (17), (17), (17), (17), (17), (17), (16), (16), (12), (11), (10), (9), (9), (9), (5), (5), (5), (5), (5), (5), (2), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
(622), (372), (247), (233), (205), (203), (161), (137), (70), (66), (40), (28), (15), (11), (9), (8), (8), (7), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling ProductDetails: References for anti-Insulin antibody (ABIN4899865) Images
Specificity Detects human and mouse Proinsulin. Does not detect mature insulin.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen E. coli-derived recombinant human Proinsulin, Phe25-Asn110, Accession # P01308
Clone 253627
Isotype IgG2a

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling ProductDetails: References for anti-Insulin antibody (ABIN4899865) Images back to top
Antigen
Alternative Name Proinsulin (INS Antibody Abstract)
Background Alternative Names: IDDM2, ILPR, insulin, IRDN, MODY10, proinsulin
Gene ID 3630
UniProt P01308
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling ProductDetails: References for anti-Insulin antibody (ABIN4899865) Images back to top
Application Notes Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details ProductDetails: References for anti-Insulin antibody (ABIN4899865) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

ProductDetails: References for anti-Insulin antibody (ABIN4899865)

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
Product cited in:

Jonatan, Spence, Method, Kofron, Sinagoga, Haataja, Arvan, Deutsch, Wells: "Sox17 regulates insulin secretion in the normal and pathologic mouse β cell." in: PLoS ONE, Vol. 9, Issue 8, pp. e104675, 2014 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Cao, Mao, Kam, Xiao, Cao, Shen, Cheng, Xu, Lee, Jiang, Xia: "PICK1 and ICA69 control insulin granule trafficking and their deficiencies lead to impaired glucose tolerance." in: PLoS biology, Vol. 11, Issue 4, pp. e1001541, 2013

Chen, Begum, Opare-Addo, Garyu, Gibson, Bothwell, Papaioannou, Herold: "Promotion of beta-cell differentiation in pancreatic precursor cells by adult islet cells." in: Endocrinology, Vol. 150, Issue 2, pp. 570-9, 2009 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Images

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling ProductDetails: References for anti-Insulin antibody (ABIN4899865) back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-Insulin (INS) (AA 25-110) antibody (ABIN4899865) Proinsulin in beta TC-6 Mouse Cell Line. Proinsulin was detected in immersion fixed b...
Immunocytochemistry (ICC) image for anti-Insulin (INS) (AA 25-110) antibody (ABIN4899865) Proinsulin in beta TC-6 Mouse Cell Line. Proinsulin was detected in immersion fixed b...