anti-Human Insulin antibody for Immunocytochemistry

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • insulin-like
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • Ins2
  • LOC100060077
Epitope
AA 25-110
27
20
15
14
12
10
6
6
5
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
933
395
283
271
263
121
49
6
6
5
5
5
4
3
2
1
1
1
1
1
1
1
Host
Mouse
821
176
55
16
2
1
Clonality
Monoclonal
Conjugate
This Insulin antibody is un-conjugated
55
42
41
27
26
26
21
21
18
17
17
17
17
17
17
16
16
12
11
10
9
9
9
5
5
5
5
5
5
2
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC)
615
362
243
222
205
199
158
136
70
66
38
29
14
11
9
9
8
7
5
4
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN4899865
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
27.67279 ABIN4900790 FACS ICC Rat IgG2a Log in to see 182410 5
27.67279 ABIN4899864 FACS ICC Biotin Mouse IgG2a AA 25-110 Log in to see 253627
1 ABIN617877 IEM ICC IF IHC Rabbit IgG Log in to see Polyclonal 44
1 ABIN4326102 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see SPM139
1 ABIN269045 ICC IF IHC (p) IHC RIA ELISA Mouse IgG1 Log in to see C-PEP-01
1 ABIN269653 DB ICC IF IHC (p) IHC RIA Mouse IgG1 Log in to see K36aC10
1 ABIN337249 FACS ICC IF IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN94408 Func ICC IHC (p) RIA ELISA Mouse IgG1 Log in to see IN-05 1
1 ABIN4326016 FACS ICC IF IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN4326030 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM139
1 ABIN4326112 FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see SPM139
1 ABIN4326028 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM139
1 ABIN4326029 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM139
1 ABIN4326104 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see SPM139
1 ABIN4326106 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG1 kappa Log in to see SPM139
1 ABIN4326108 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG1 kappa Log in to see SPM139
1 ABIN4326107 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG1 kappa Log in to see SPM139
1 ABIN4326111 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG1 kappa Log in to see SPM139
1 ABIN4326109 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG1 kappa Log in to see SPM139
1 ABIN4326114 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 550 Mouse IgG1 kappa Log in to see SPM139

Top referenced anti-Insulin antibody for Immunocytochemistry

Similar anti-Insulin Antibodies

Application / Reactivity Mouse (Murine)
Cytometry by Time of Flight (CyTOF) 10 Antibodies
ELISA 87 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 138 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 61 Antibodies
Immunoelectron Microscopy (IEM) 1 Antibodies
Immunofluorescence (IF) 75 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 31 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 92 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 153 Antibodies
Immunoprecipitation (IP) 15 Antibodies
Protein Assay (PrA) 2 Antibodies
Radioimmunoassay (RIA) 3 Antibodies
Western Blotting (WB) 87 Antibodies

General

Antigen Insulin (INS) Antibodies
Epitope AA 25-110
(27), (20), (15), (14), (12), (10), (6), (6), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(933), (395), (283), (271), (263), (121), (49), (6), (6), (5), (5), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(821), (176), (55), (16), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is un-conjugated
(55), (42), (41), (27), (26), (26), (21), (21), (18), (17), (17), (17), (17), (17), (17), (16), (16), (12), (11), (10), (9), (9), (9), (5), (5), (5), (5), (5), (5), (2), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC)
(615), (362), (243), (222), (205), (199), (158), (136), (70), (66), (38), (29), (14), (11), (9), (9), (8), (7), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling ProductDetails: References for anti-Insulin antibody (ABIN4899865) Images
Specificity Detects human and mouse Proinsulin. Does not detect mature insulin.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen E. coli-derived recombinant human Proinsulin, Phe25-Asn110, Accession # P01308
Clone 253627
Isotype IgG2a

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling ProductDetails: References for anti-Insulin antibody (ABIN4899865) Images back to top
Antigen
Alternative Name Proinsulin (INS Antibody Abstract)
Background Alternative Names: IDDM2, ILPR, insulin, IRDN, MODY10, proinsulin
Gene ID 3630
UniProt P01308
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling ProductDetails: References for anti-Insulin antibody (ABIN4899865) Images back to top
Application Notes Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details ProductDetails: References for anti-Insulin antibody (ABIN4899865) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

ProductDetails: References for anti-Insulin antibody (ABIN4899865)

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
Product cited in:

Jonatan, Spence, Method, Kofron, Sinagoga, Haataja, Arvan, Deutsch, Wells: "Sox17 regulates insulin secretion in the normal and pathologic mouse β cell." in: PLoS ONE, Vol. 9, Issue 8, pp. e104675, 2014 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Cao, Mao, Kam, Xiao, Cao, Shen, Cheng, Xu, Lee, Jiang, Xia: "PICK1 and ICA69 control insulin granule trafficking and their deficiencies lead to impaired glucose tolerance." in: PLoS biology, Vol. 11, Issue 4, pp. e1001541, 2013

Chen, Begum, Opare-Addo, Garyu, Gibson, Bothwell, Papaioannou, Herold: "Promotion of beta-cell differentiation in pancreatic precursor cells by adult islet cells." in: Endocrinology, Vol. 150, Issue 2, pp. 570-9, 2009 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Images

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling ProductDetails: References for anti-Insulin antibody (ABIN4899865) back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-Insulin antibody (INS) (AA 25-110) (ABIN4899865) Proinsulin in beta TC-6 Mouse Cell Line. Proinsulin was detected in immersion fixed b...