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anti-Pig (Porcine) Insulin antibody for Immunoassay

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • AA986540
  • IDDM2
  • ILPR
  • Ins-2
  • ins1
  • ins1-a
  • InsII
  • Insulin
  • IRDN
  • Mody
  • Mody4
  • MODY10
  • proinsulin
  • xins
  • zgc:109842
Reactivity
Pig (Porcine)
563
134
134
97
78
25
4
4
3
3
3
3
2
2
1
1
1
1
1
1
1
Host
Guinea Pig
447
177
56
2
2
Clonality
Polyclonal
Conjugate
This Insulin antibody is conjugated to FITC
37
27
25
12
10
10
6
5
5
5
5
5
5
5
5
1
1
1
1
Application
Immunoassay (IA)
385
181
140
124
68
56
49
42
39
36
26
13
12
11
9
8
7
6
5
5
4
3
1
1
1
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Catalog No. ABIN458552
$ 458.18
Plus shipping costs $45.00

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Application / Reactivity Pig (Porcine)
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Enzyme Immunoassay (EIA) 12 Antibodies
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Functional Studies (Func) 13 Antibodies
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Immunohistochemistry (Frozen Sections) (IHC (fro)) 29 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 56 Antibodies
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General

Antigen Insulin (INS) Antibodies
Reactivity Pig (Porcine)
(563), (134), (134), (97), (78), (25), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Guinea Pig
(447), (177), (56), (2), (2)
Clonality
Polyclonal
Conjugate This Insulin antibody is conjugated to FITC
(37), (27), (25), (12), (10), (10), (6), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1), (1)
Application Immunoassay (IA)
(385), (181), (140), (124), (68), (56), (49), (42), (39), (36), (26), (13), (12), (11), (9), (8), (7), (6), (5), (5), (4), (3), (1), (1), (1)
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling Images
Specificity Inter-species cross-reactivity is a normal feature of antibodies to mammalian proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has not been tested for cross-reactivity.
Characteristics Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal guinea pig antiserum to swine insulin
Purification Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG (7S) fraction by DEAE-chromatography.
Immunogen Insulin is a hormone produced by the beta cells of the islets of Langerhans in the pancreas. It plays an important role in the metabolism of carbohydrate by its effects on the storage of glycogen in the liver, the conversion of carbohydrate to fat, and the oxidation of glucose by the peripheral tissues. Insulin has a molecular weight of 6,000 and contains two peptide chains linked by two disulphide bonds. The hormone is isolated from porcine pancreas. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background The reactivity of the antiserum is directed to insulin as tested by ELISA and immunoblotting. No reaction was obtained with other swine serum proteins.
Pathways NF-kappaB Signaling, RTK Signaling

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling Images back to top
Application Notes The immunoconjugate is intended for use in different types of highly sensitive fluorescence immunoassays on appropriately treated cell and tissue substrates. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Working dilutions are usually between 1:10 and 1:40.
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details Images back to top
Format Lyophilized
Concentration IgG protein concentration 10 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) approximately 2.7. No foreign proteins added.
Buffer Purified hyperimmune guinea pig IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
Preservative Without preservative
Storage 4 °C/-20 °C
Storage Comment The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile di stilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.