anti-Rabbit Insulin antibody for Immunohistochemistry (Frozen Sections)

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • insulin-like
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • Ins2
  • LOC100060077
Reactivity
Cat (Feline), Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
941
398
284
272
262
120
49
5
5
4
4
4
4
3
2
1
1
1
1
1
1
1
Host
Mouse
829
179
55
16
3
1
Clonality
Monoclonal
Conjugate
This Insulin antibody is un-conjugated
55
42
41
27
26
26
21
21
18
17
17
17
17
17
17
16
16
12
11
10
9
9
9
5
5
5
5
5
5
2
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
617
373
244
228
206
204
161
138
69
66
38
29
14
11
9
9
8
7
5
4
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN114751
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1804387 IHC (fro) IHC (p) ELISA Mouse IgG1 Log in to see E2E3
1 ABIN114750 IHC (fro) IHC (p) Mouse IgG1 Log in to see K36aC10
1 ABIN2475104 IHC (fro) IHC (p) ELISA Mouse IgG1 Log in to see E2E3 1
1 ABIN1868716 ICC IHC (fro) IHC (p) ELISA WB Guinea Pig IgG Log in to see Polyclonal
1 ABIN2963151 IHC (fro) IHC (p) ELISA Mouse IgG1 full length Log in to see E2E3
1 ABIN4326128 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG1 kappa Log in to see SPM531
1 ABIN4326126 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG1 kappa Log in to see SPM531
1 ABIN4326121 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see SPM531
1 ABIN4326033 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM531
1 ABIN4326056 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326058 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326067 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326069 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326068 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326070 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326074 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326073 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326077 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326081 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326082 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)

Top referenced anti-Insulin antibody for Immunohistochemistry (Frozen Sections)

Similar anti-Insulin Antibodies

Application / Reactivity Human Cow (Bovine) Pig (Porcine) Rabbit Rat (Rattus) Horse (Equine) Dog (Canine) Cat (Feline)
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies 4 Antibodies 5 Antibodies 1 Antibodies 1 Antibodies
Cytometry by Time of Flight (CyTOF) 14 Antibodies 8 Antibodies 12 Antibodies 8 Antibodies 4 Antibodies
Western Blotting (WB) 191 Antibodies 110 Antibodies 97 Antibodies 51 Antibodies 1 Antibodies 62 Antibodies 1 Antibodies 1 Antibodies
Radioimmunoassay (RIA) 32 Antibodies

General

Antigen Insulin (INS) Antibodies
Reactivity Cat (Feline), Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
(941), (398), (284), (272), (262), (120), (49), (5), (5), (4), (4), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(829), (179), (55), (16), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is un-conjugated
(55), (42), (41), (27), (26), (26), (21), (21), (18), (17), (17), (17), (17), (17), (17), (16), (16), (12), (11), (10), (9), (9), (9), (5), (5), (5), (5), (5), (5), (2), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(617), (373), (244), (228), (206), (204), (161), (138), (69), (66), (38), (29), (14), (11), (9), (9), (8), (7), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling ProductDetails: References for anti-Insulin antibody (ABIN114751) Images
Specificity This antibody recognizes purified Insulin from the pancreas of Human, Bovine, Horse, Sheep as well as Proinsulin from Human. Cross reaction has been observed with insulin containing cells in fixed sections of pancreas from Human, Porcine, Dog, Rabbit, Bovine, Sheep, Rat, Guinea Pig and Cat.
Cellular Localization: Cytoplasmic.Cellular Localization
Purification Supernatant
Immunogen Human insulin.
Clone K36aC10
Isotype IgG1

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling ProductDetails: References for anti-Insulin antibody (ABIN114751) Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.Synonyms: INS
Gene ID 3630
NCBI Accession NP_000198
UniProt P01308
Research Area Neurology, Diabetes, Hormones
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling ProductDetails: References for anti-Insulin antibody (ABIN114751) Images back to top
Application Notes Immunohistochemistry on Formalin/Paraffin Sections: Use a dilution of 1/25-1/50 in anABC method (30 minutes at room temperature). Recommended Positive Control: Pancreas.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details ProductDetails: References for anti-Insulin antibody (ABIN114751) Images back to top
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-Insulin antibody (ABIN114751)

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
Background publications

Madsen: "Proinsulin-specific monoclonal antibodies. Immunocytochemical application as beta-cell markers and as probes for conversion." in: Diabetes, Vol. 36, Issue 10, pp. 1203-11, 1987

Keilacker, Dietz, Witt, Woltanski, Berling, Ziegler: "Kinetic properties of monoclonal insulin antibodies." in: Biomedica biochimica acta, Vol. 45, Issue 8, pp. 1093-102, 1986