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|Antigen||Insulin Receptor (INSR) Antibodies|
|Epitope||AA 1006-1144 Alternatives|
|Reactivity||Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Insulin Receptor antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-Insulin Receptor AntibodyTarget Details Insulin Receptor Application Details Handling ProductDetails: References for anti-Insulin Receptor antibody (ABIN967752) Images
|Cross-Reactivity||Mouse (Murine), Rat (Rattus)|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Human Insulin pro-receptor beta subunit 1006-1144|
Target Details Insulin ReceptorProduct Details anti-Insulin Receptor Antibody Application Details Handling ProductDetails: References for anti-Insulin Receptor antibody (ABIN967752) Images back to top
|Alternative Name||CD220 (INSR Antibody Abstract)|
Insulin Receptor (IR) is a transmembrane receptor tyrosine kinase which, upon insulin binding, initiates a cascade of events, including autophosphorylation, phosphorylation of cellular protein substrates, glucose transport, and glycogen synthesis. IR is synthesized as a large glycosylated precursor that is cleaved upon maturation into a 130 kDa alpha-subunit with kinase activity and a 95 kDa beta-subunit. The active Insulin Receptor is a heterotetramer of homologus alpha and beta subunits joined by disulfide bonds. Among the major cytosolic substrates of the Insulin Receptor are IRS-1 and -2, beta-Adrenergic receptor, and pp15 (adipocyte lipid-binding protein, ALBP). Autophosphorylation of the IR recruits IRS-1 and -2 to the phosphotyrosines. Subsequently, the phosphorylated IRS-1 and -2 act as docking sites for other signaling proteins like PI3-Kinase, Shc, PTP1D, Nck, etc. In addition, the phosphatase LAR is tightly associated with the IR and LAR becomes activated after insulin stimulation dephosphorylating the IR and its substrates. Therefore, LAR provides a turn-off mechanism in insulin signaling. This antibody is routinely tested by western blot analysis.
Synonyms: Insulin Receptor beta
|Molecular Weight||95 kDa|
|Pathways||NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity|
Application DetailsProduct Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Handling ProductDetails: References for anti-Insulin Receptor antibody (ABIN967752) Images back to top
Related Products: ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Application Details ProductDetails: References for anti-Insulin Receptor antibody (ABIN967752) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20° C.|
ProductDetails: References for anti-Insulin Receptor antibody (ABIN967752)Product Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Application Details Handling Images back to top
|Product cited in:||
Shumay, Song, Wang, Malbon: "pp60Src mediates insulin-stimulated sequestration of the beta(2)-adrenergic receptor: insulin stimulates pp60Src phosphorylation and activation." in: Molecular biology of the cell, Vol. 13, Issue 11, pp. 3943-54, 2002
Zundel, Swiersz, Giaccia: "Caveolin 1-mediated regulation of receptor tyrosine kinase-associated phosphatidylinositol 3-kinase activity by ceramide." in: Molecular and cellular biology, Vol. 20, Issue 5, pp. 1507-14, 2000
Frattali, Treadway, Pessin: "Insulin/IGF-1 hybrid receptors: implications for the dominant-negative phenotype in syndromes of insulin resistance." in: Journal of cellular biochemistry, Vol. 48, Issue 1, pp. 43-50, 1992
Yip, Jack: "Insulin receptors are bivalent as demonstrated by photoaffinity labeling." in: The Journal of biological chemistry, Vol. 267, Issue 19, pp. 13131-4, 1992
Ebina, Ellis, Jarnagin, Edery, Graf, Clauser, Ou, Masiarz, Kan, Goldfine: "The human insulin receptor cDNA: the structural basis for hormone-activated transmembrane signalling." in: Cell, Vol. 40, Issue 4, pp. 747-58, 1985
ImagesProduct Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Application Details Handling ProductDetails: References for anti-Insulin Receptor antibody (ABIN967752) back to top