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|Application / Reactivity||Human|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||7 Antibodies|
|Flow Cytometry (FACS)||4 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Immunocytochemistry (ICC)||10 Antibodies|
|Immunofluorescence (IF)||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||23 Antibodies|
|Immunohistochemistry (IHC)||18 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||2 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||23 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies|
|Intracellular Staining (ICS)||2 Antibodies|
|Western Blotting (WB)||131 Antibodies|
|Antigen||Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) Antibodies|
|Conjugate||This IRAK4 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-IRAK4 AntibodyTarget Details IRAK4 Application Details Handling References for anti-IRAK4 Antibody (ABIN500044) Images
|Specificity||IRAK-4 antibody was raised against a synthetic peptide corresponding to amino acids at the carboxy terminus of human IRAK-4. Anti-IRAK-4 has no cross response to other IRAKs.|
|Purification||Affinity chromatography purified via peptide column|
|Immunogen||Human IRAK4 Peptide|
Target Details IRAK4Product Details anti-IRAK4 Antibody Application Details Handling References for anti-IRAK4 Antibody (ABIN500044) Images back to top
|Alternative Name||IRAK4 (IRAK4 Antibody Abstract)|
|Background||Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular responses through IL-1 receptor (IL-1R) and Toll-like receptors (TLR). IL-1R-associated kinases (IRAK, IRAK2, and IRAK-M) regulate the activation of NF-kappaB and MAP kinase (MAPK) by IL-1R/TLR (1-3). A novel member in the IRAK/Pelle family was recently identified and designated IRAK-4 (4,5). Overexpression of IRAK-4 activates NF-kB and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges (5,6). Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.Synonyms: IRAK-4, Interleukin-1 receptor-associated kinase-like, NY-REN-64|
|Research Area||Immunology, Innate Immunity, Atherosclerosis, Cytokines, Receptors, Inflammation|
|Pathways||NF-kappaB Signaling, TLR Signaling|
Application DetailsProduct Details anti-IRAK4 Antibody Target Details IRAK4 Handling References for anti-IRAK4 Antibody (ABIN500044) Images back to top
ELISA. Western Blot: 1 to 2 μg/mL. A 50 kDa band can be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IRAK4 Antibody Target Details IRAK4 Application Details References for anti-IRAK4 Antibody (ABIN500044) Images back to top
|Buffer||PBS containing 0.02 % sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
References for anti-IRAK4 Antibody (ABIN500044)Product Details anti-IRAK4 Antibody Target Details IRAK4 Application Details Handling Images back to top
Muzio, Ni, Feng et al.: "IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling." in: Science (New York, N.Y.), Vol. 278, Issue 5343, pp. 1612-5, 1997 (PubMed).
Cao, Henzel, Gao: "IRAK: a kinase associated with the interleukin-1 receptor." in: Science (New York, N.Y.), Vol. 271, Issue 5252, pp. 1128-31, 1996 (PubMed).
ImagesProduct Details anti-IRAK4 Antibody Target Details IRAK4 Application Details Handling References for anti-IRAK4 Antibody (ABIN500044) back to top