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anti-Human IRAK4 antibody for Immunofluorescence

Recommended IRAK4 Antibody (supplied by: Log in to see )

Antigen
Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) Antibodies
  • 8430405M07Rik
  • 9330209D03Rik
  • IPD1
  • ipd1
  • irak-4
  • IRAK-4
  • irak4
  • IRAK4
  • ny-ran-64
  • NY-REN-64
  • ny-ren-64
  • ren64
  • REN64
  • zgc:55553
Reactivity
Human
182
49
19
7
2
2
2
1
1
1
1
1
Host
Rabbit
137
30
26
Clonality
Polyclonal
Conjugate
This IRAK4 antibody is un-conjugated
9
8
7
6
5
5
4
3
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
147
87
27
22
22
11
9
6
6
6
4
3
2
2
1
1
Supplier
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Catalog No. ABIN500044
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32040194 ABIN526492 IF ELISA Mouse IgG2a kappa AA 255-351 Log in to see 1C3
0.0010840179 ABIN302830 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0010840179 ABIN208876 IF WB Rabbit N-Term Log in to see Polyclonal
0.0010840179 ABIN2290777 IF WB Rabbit IgG N-Term Log in to see Polyclonal
0.0010840179 ABIN2959777 ICC IF ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010840179 ABIN4327518 ELISA ICC IF WB Rabbit Log in to see Polyclonal
0.0010840179 ABIN786736 ICC IF Alexa Fluor 546 Mouse IgG1 Log in to see 7D8
0.0010840179 ABIN786735 ICC IF Alexa Fluor 488 Mouse IgG1 Log in to see 7D8
0.0010840179 ABIN2772632 ELISA (Capture) IF ELISA Mouse IgG2a, kappa Log in to see

Top referenced anti-IRAK4 antibody for Immunofluorescence

Similar anti-IRAK4 Antibodies

Application / Reactivity Human
ELISA 80 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 6 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 11 Antibodies
Immunofluorescence (IF) 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 21 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 25 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Intracellular Staining (ICS) 2 Antibodies
Western Blotting (WB) 138 Antibodies

General

Antigen Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) Antibodies
Reactivity Human
(182), (49), (19), (7), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(137), (30), (26)
Clonality
Polyclonal
Conjugate This IRAK4 antibody is un-conjugated
(9), (8), (7), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(147), (87), (27), (22), (22), (11), (9), (6), (6), (6), (4), (3), (2), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-IRAK4 Antibody

Target Details IRAK4 Application Details Handling References for anti-IRAK4 Antibody (ABIN500044) Images
Specificity IRAK-4 antibody was raised against a synthetic peptide corresponding to amino acids at the carboxy terminus of human IRAK-4. Anti-IRAK-4 has no cross response to other IRAKs.
Purification Affinity chromatography purified via peptide column
Immunogen Human IRAK4 Peptide
Isotype IgG

Target Details IRAK4

Product Details anti-IRAK4 Antibody Application Details Handling References for anti-IRAK4 Antibody (ABIN500044) Images back to top
Antigen
Alternative Name IRAK4 (IRAK4 Antibody Abstract)
Background Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular responses through IL-1 receptor (IL-1R) and Toll-like receptors (TLR). IL-1R-associated kinases (IRAK, IRAK2, and IRAK-M) regulate the activation of NF-kappaB and MAP kinase (MAPK) by IL-1R/TLR (1-3). A novel member in the IRAK/Pelle family was recently identified and designated IRAK-4 (4,5). Overexpression of IRAK-4 activates NF-kB and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges (5,6). Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.Synonyms: IRAK-4, Interleukin-1 receptor-associated kinase-like, NY-REN-64
Gene ID 51135
UniProt Q9NWZ3
Research Area Immunology, Innate Immunity, Atherosclerosis, Cytokines, Receptors, Inflammation
Pathways NF-kappaB Signaling, TLR Signaling

Application Details

Product Details anti-IRAK4 Antibody Target Details IRAK4 Handling References for anti-IRAK4 Antibody (ABIN500044) Images back to top
Application Notes ELISA. Western Blot: 1 to 2 μg/mL. A 50 kDa band can be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IRAK4 Antibody Target Details IRAK4 Application Details References for anti-IRAK4 Antibody (ABIN500044) Images back to top
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-IRAK4 Antibody (ABIN500044)

Product Details anti-IRAK4 Antibody Target Details IRAK4 Application Details Handling Images back to top
Background publications

Muzio, Ni, Feng, Dixit: "IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling." in: Science (New York, N.Y.), Vol. 278, Issue 5343, pp. 1612-5, 1997

Cao, Henzel, Gao: "IRAK: a kinase associated with the interleukin-1 receptor." in: Science (New York, N.Y.), Vol. 271, Issue 5252, pp. 1128-31, 1996

Images

Product Details anti-IRAK4 Antibody Target Details IRAK4 Application Details Handling References for anti-IRAK4 Antibody (ABIN500044) back to top
Supplier Images
Immunofluorescence (IF) image for anti-IRAK4 antibody (Interleukin-1 Receptor-Associated Kinase 4) (ABIN500044) Immunocytochemistry of IRAK-4 in K562 cells with AP30442PU-N IRAK-4 antibody at 10 μg...
Western Blotting (WB) image for anti-IRAK4 antibody (Interleukin-1 Receptor-Associated Kinase 4) (ABIN500044) Western blot analysis of IRAK-4 in HeLa cell lysate with AP30442PU-N IRAK-4 antibody ...