anti-Rat (Rattus) NGFB antibody for Western Blotting

Recommended NGFB Antibody (supplied by: Log in to see )

Antigen
Nerve Growth Factor beta (NGFB) Antibodies
  • NGFB
  • ngfb
  • ns:zft386
  • xx:zft386
  • zgc:109730
  • hsan5
  • beta-ngf
  • Ngfb
  • Beta-NGF
  • HSAN5
  • beta-NGF
  • nerve growth factor (beta polypeptide)
  • beta-Nerve growth factor
  • nerve growth factor
  • NGF
  • ngf
  • FPV072
  • FPV076
  • Ngf
Epitope
AA 162-173, N-Term
15
14
11
7
5
4
2
2
2
2
2
1
1
1
1
Reactivity
Human, Rat (Rattus)
176
110
86
13
4
3
3
2
1
Host
Rabbit
137
57
10
3
2
2
Clonality
Polyclonal
Conjugate
This NGFB antibody is un-conjugated
18
7
7
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
126
123
57
29
18
17
13
13
10
8
6
4
2
2
1
1
1
1
1
1
1
Options
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Catalog No. ABIN3043754
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1474795 ABIN3185895 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
3.1474795 ABIN1108447 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
3.1474795 ABIN498573 IHC (p) WB Rabbit Log in to see Polyclonal
3.1474795 ABIN1584876 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
3.1474795 ABIN3020812 IHC WB Rabbit IgG Log in to see Polyclonal
3.1474795 ABIN3031957 WB Rabbit IgG N-Term Log in to see Polyclonal
3.1474795 ABIN2403522 IHC WB Rabbit IgG Log in to see Polyclonal
3.1474795 ABIN5548915 ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN724430 IF (p) IHC (p) WB Rabbit IgG AA 150-200 Log in to see Polyclonal 4
1 ABIN1495058 IHC (p) WB Rabbit N-Term Log in to see Polyclonal
1 ABIN600023 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1453650 IHC ELISA WB Rabbit IgG AA 33-82 Log in to see Polyclonal
1 ABIN1584875 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN724439 IHC (p) WB HRP Rabbit IgG AA 150-200 Log in to see Polyclonal
1 ABIN1495097 IHC (p) IP WB Sepharose Rabbit N-Term Log in to see Polyclonal
1 ABIN1495096 IHC (p) IP WB Magnetic Particles Rabbit N-Term Log in to see Polyclonal
1 ABIN328939 IHC ELISA WB Rabbit Log in to see Polyclonal
1 ABIN350507 InhA IHC WB Rabbit Log in to see Polyclonal 4
1 ABIN350516 InhA IHC WB Rabbit Log in to see Polyclonal 4
1 ABIN724432 IHC (p) WB Biotin Rabbit IgG AA 150-200 Log in to see Polyclonal

General

Antigen Nerve Growth Factor beta (NGFB) Antibodies
Epitope AA 162-173, N-Term
(15), (14), (11), (7), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(176), (110), (86), (13), (4), (3), (3), (2), (1)
Host Rabbit
(137), (57), (10), (3), (2), (2)
Clonality
Polyclonal
Conjugate This NGFB antibody is un-conjugated
(18), (7), (7), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(126), (123), (57), (29), (18), (17), (13), (13), (10), (8), (6), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-NGFB Antibody

Target Details NGFB Application Details Handling References for anti-NGFB antibody (ABIN3043754) Images
Purpose Rabbit IgG polyclonal antibody for Beta-nerve growth factor(NGF) detection. Tested with WB in Human,Mouse,Rat.
Sequence EVNINNSVFK QY
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for Beta-nerve growth factor(NGF) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: nerve growth factor(beta polypeptide)
Protein Name: Beta-nerve growth factor(Beta-NGF)
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human NGF(162-173aa EVNINNSVFKQY), identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
Isotype IgG

Target Details NGFB

Product Details anti-NGFB Antibody Application Details Handling References for anti-NGFB antibody (ABIN3043754) Images back to top
Antigen
Alternative Name NGF (NGFB Antibody Abstract)
Background Nerve growth factor is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. The nucleotide sequence of human and mouse beta-NGF are very similar. The beta-subunits of nerve growth factor(NGFB) have been assigned to mouse chromosome 3 and human chromosome 1p22. The human gene for the beta subunit of nerve growth factor is located on the proximal short arm of chromosome 1. A mutation in the nerve growth factor beta gene(NGFB) causes loss of pain perception.

Synonyms: Beta nerve growth factor antibody|Beta nerve growth factor precursor antibody|Beta NGF antibody|Beta-nerve growth factor antibody|Beta-NGF antibody|HSAN5 antibody|MGC161426 antibody|MGC161428 antibody|Nerve growth factor beta antibody|Nerve growth factor beta polypeptide antibody|Nerve growth factor beta subunit antibody|NGF antibody|NGF B antibody|NGF_HUMAN antibody|NGFB antibody|NID67 antibody
UniProt P01138
Research Area Neurology, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Regulation of Cell Size

Application Details

Product Details anti-NGFB Antibody Target Details NGFB Handling References for anti-NGFB antibody (ABIN3043754) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions For Research Use only

Handling

Product Details anti-NGFB Antibody Target Details NGFB Application Details References for anti-NGFB antibody (ABIN3043754) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-NGFB antibody (ABIN3043754)

Product Details anti-NGFB Antibody Target Details NGFB Application Details Handling Images back to top
Product cited in:

Luo, Liu, Chen, Wang, Zeng, Chen, Cheng et al.: "Nerve growth factor and inducible nitric oxide synthase expression in the mesencephalon and diencephalon, as well as visual- and auditory-related nervous tissues, in a macaque model of type 2 ..." in: Neural regeneration research, Vol. 7, Issue 4, pp. 302-7, 2015

Lu, Liu, Zhu, Xu: "Expression of nerve growth factor and hypoxia inducible factor-1? and its correlation with angiogenesis in non-small cell lung cancer." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 34, Issue 3, pp. 359-62, 2014

Li, Ma, Yi, Wang, Li, Liu, Sun, Chen, Yu, Li, Chen, Zhou: "The interactions between nerve growth factor and gonadotrophins in bovine oviduct." in: Animal reproduction science, Vol. 149, Issue 3-4, pp. 117-23, 2014

Huang, Zhang, Xu, Li, Zhao: "Expression of nerve growth factor in the prostate of male rats in response to chronic stress and sympathetic denervation." in: Experimental and therapeutic medicine, Vol. 8, Issue 4, pp. 1237-1240, 2014

Zhao, Ding, Guo, Chen: "Intrathecal lidocaine neurotoxicity: combination with bupivacaine and ropivacaine and effect of nerve growth factor." in: Life sciences, Vol. 112, Issue 1-2, pp. 10-21, 2014

Li, Shu, Tang, Deng, Fu, Lv: "?-Nerve growth factor attenuates hepatocyte injury induced by D-galactosamine in vitro via TrkA NGFR." in: Molecular medicine reports, Vol. 8, Issue 3, pp. 813-7, 2013

Sun, Guo, Zheng, Zhang, Li, Liu, Yin, Yang, Shu: "Increased expression of TRPV1 in the cortex and hippocampus from patients with mesial temporal lobe epilepsy." in: Journal of molecular neuroscience : MN, Vol. 49, Issue 1, pp. 182-93, 2012

Wang, Zhao, Fan, Gong, Li, Qi, Liu: "Research on the mechanism of Zuogui Pill and Yougui Pill in promoting axonal regeneration in model rats of autoimmune encephalomyelitis." in: Chinese journal of integrative medicine, Vol. 16, Issue 2, pp. 167-72, 2010

Images

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Supplier Images
Western Blotting (WB) image for anti-Nerve Growth Factor beta (NGFB) (AA 162-173), (N-Term) antibody (ABIN3043754) Anti-NGF antibody, Western blotting WB: Rat Brain Tissue Lysate
Western Blotting (WB) image for anti-Nerve Growth Factor beta (NGFB) (AA 162-173), (N-Term) antibody (ABIN3043754) Anti-NGF antibody, Western blotting Lane 1: Recombinant Human NGFB Protein 10ng Lane ...