anti-Cow (Bovine) PKC zeta antibody for Western Blotting

Recommended PKC zeta Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, zeta (PRKCZ) Antibodies
  • TPKC
  • PKCzeta
  • prkcz-A
  • pkc-zeta
  • PRKCZ
  • pkc2
  • PKC-ZETA
  • PKC2
  • AI098070
  • C80388
  • Pkcz
  • R74924
  • aPKCzeta
  • zetaPKC
  • 14-3-3-zetaisoform
  • r14-3-3
  • pkcz
  • si:ch211-150o23.4
  • protein kinase C, zeta
  • prkcz
  • PRKCZ
  • Prkcz
Epitope
N-Term
42
31
17
14
12
8
6
5
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
168
109
91
8
7
6
3
3
3
2
2
2
2
2
1
Host
Rabbit
194
4
1
Clonality
Polyclonal
Conjugate
This PKC zeta antibody is un-conjugated
9
7
5
4
4
4
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
140
92
89
39
31
15
13
8
6
3
3
2
2
2
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN2775614
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN225191 WB Rabbit IgG N-Term Log in to see Polyclonal

General

Antigen Protein Kinase C, zeta (PRKCZ) Antibodies
Epitope N-Term
(42), (31), (17), (14), (12), (8), (6), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(168), (109), (91), (8), (7), (6), (3), (3), (3), (2), (2), (2), (2), (2), (1)
Host Rabbit
(194), (4), (1)
Clonality
Polyclonal
Conjugate This PKC zeta antibody is un-conjugated
(9), (7), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(140), (92), (89), (39), (31), (15), (13), (8), (6), (3), (3), (2), (2), (2), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-PKC zeta Antibody

Target Details PKC zeta Application Details Handling References for anti-PKC zeta antibody (ABIN2775614) Images
Blocking Peptide Blocking peptide for this product available: ABIN982222
Sequence MDSVMPSQEP PVDDKNEDAD LPSEETDGIA YISSSRKHDS IKDDSEDLKP
Predicted Reactivity Cow: 77%, Dog: 75%, Guinea Pig: 75%, Horse: 75%, Human: 100%, Mouse: 92%, Rabbit: 79%, Rat: 92%
Characteristics This is a rabbit polyclonal antibody against PRKCZ. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human PRKCZ

Target Details PKC zeta

Product Details anti-PKC zeta Antibody Application Details Handling References for anti-PKC zeta antibody (ABIN2775614) Images back to top
Antigen
Alternative Name PRKCZ (PRKCZ Antibody Abstract)
Background Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC.Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.
Alias Symbols: PKC2, PKC-ZETA
Protein Interaction Partner: MAPK7, PRKCZ, AKT2, AKT1, Sqstm1, PARD6A, NUMB, NPM1, HIST1H1B, CFL1, C1QBP, HSP90AA1, NCF1, MARCKS, PPP1R14A, HIST1H1A, UBC, VHL, LRRK2, AJUBA, PARD6B, PAWR, Smurf1, Smurf2, Pard3, HIST3H3, CD44, NCOA3, TFF1, RELA, ESR1, MBP, TRIM41, PIAS4, CCDC115, ZNF7
Protein Size: 409
Molecular Weight 45 kDa
Gene ID 5590
NCBI Accession NM_001033581, NP_001028753
UniProt Q05513
Pathways NF-kappaB Signaling, RTK Signaling, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling

Application Details

Product Details anti-PKC zeta Antibody Target Details PKC zeta Handling References for anti-PKC zeta antibody (ABIN2775614) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 409 AA

Restrictions For Research Use only

Handling

Product Details anti-PKC zeta Antibody Target Details PKC zeta Application Details References for anti-PKC zeta antibody (ABIN2775614) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PKC zeta antibody (ABIN2775614)

Product Details anti-PKC zeta Antibody Target Details PKC zeta Application Details Handling Images back to top
Background publications

Xie, Dong, Zhang, Cui, Cohen, Riek, Neumann, Schlattner, Zou: "Activation of protein kinase C zeta by peroxynitrite regulates LKB1-dependent AMP-activated protein kinase in cultured endothelial cells." in: The Journal of biological chemistry, Vol. 281, Issue 10, pp. 6366-75, 2006

Images

Product Details anti-PKC zeta Antibody Target Details PKC zeta Application Details Handling References for anti-PKC zeta antibody (ABIN2775614) back to top
Supplier Images
Western Blotting (WB) image for anti-Protein Kinase C, zeta (PRKCZ) (N-Term) antibody (ABIN2775614) WB Suggested Anti-PRKCZ Antibody Titration: 0.2-1 ug/ml
Positive Control...