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Rat (Rattus) TNFRSF11A ELISA Kit

Recommended TNFRSF11A Product (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A) ELISA Kits
  • CD265
  • FEO
  • LOH18CR1
  • Ly109
  • mRANK
  • ODFR
  • OFE
  • OPTB7
  • OSTS
  • PDB2
  • Rank
  • RANK
  • RGD1563614
  • TNFRSF11A
  • TRANCE-R
  • TRANCER
Epitope
AA 30-213
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
8
6
6
1
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
62.5-4000 pg/mL
Minimum Detection Limit
62.5 pg/mL
Application
ELISA
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Catalog No. ABIN1889423
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN1113959 Sandwich ELISA Serum, Plasma, Biological Fluids 0.16-10 ng/mL 0.16 ng/mL Log in to see
0.0012117105 ABIN1095380 Sandwich ELISA Serum, Plasma, Tissue Homogenate 0.45-30 ng/mL 0.45 ng/mL Log in to see
0.0012117105 ABIN2748781 Sandwich ELISA Cell Culture Supernatant, Serum 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012117105 ABIN2685706 Sandwich ELISA Cell Culture Supernatant, Serum 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012117105 ABIN2538519 Sandwich ELISA Tissue Homogenate, Cell Lysate, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012117105 ABIN2951036 0.156-10 ng/mL 0.156 ng/mL Log in to see

Top referenced TNFRSF11A ELISA Kit for ELISA

  • Rat (Rattus) TNFRSF11A ELISA Kit for Sandwich ELISA - ABIN1889423 (1 Publications): Darnay, Haridas, Ni, Moore, Aggarwal et al.: Characterization of the intracellular domain of receptor activator of NF-kappaB (RANK). Interaction with tumor necrosis factor receptor-associated factors and activation of NF-kappab and c-Jun ... in The Journal of biological chemistry 1998 (PubMed)

Similar TNFRSF11A ELISA Kits

Application / Reactivity Rat (Rattus)
ELISA 7 ELISA Kits

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A) ELISA Kits
Epitope AA 30-213
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(8), (6), (6), (1), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 62.5-4000 pg/mL
Minimum Detection Limit 62.5 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details TNFRSF11A ELISA Kit

Target details Application Details Handling References for TNFRSF11A Kit (ABIN1889423) Images
Purpose For quantitative detection of rat RANK in cell culture supernates and serum.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum
Detection Method Colorimetric
Specificity Natural and recombinant rat RANK
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- rat RANK antibod - 1
  • Lyophilized recombinant rat RANK standar - 10ng/tubex2
  • Biotinylated anti- rat RANK antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: Q30-P213

Target details

Product Details TNFRSF11A ELISA Kit Application Details Handling References for TNFRSF11A Kit (ABIN1889423) Images back to top
Antigen
Alternative Name TNFRSF11A (TNFRSF11A ELISA Kit Abstract)
Background Receptor Activator of Nuclear Factor κ B (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain(residues 234-616), which contain three putative TRAF-binding domains(termed I, II, and III). RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK. The standard in this kit is recombinant human RANK with the sequence of Q29-G213 aa. It is a dipolymer which compose of two chains, and the molecular weight of each is 48kda.
Gene ID 498206
UniProt F1M8Z6
Pathways NF-kappaB Signaling

Application Details

Product Details TNFRSF11A ELISA Kit Target details Handling References for TNFRSF11A Kit (ABIN1889423) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol rat RANK ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for RANK has been precoated onto 96-well plates. Standards(NSO, Q30-P213) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for RANK is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat RANK amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat RANK standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat RANK standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 880, Standard deviation: 36.96, CV(%): 4.2
  • Sample 2: n=16, Mean(pg/ml): 1954, Standard deviation: 107.5, CV(%): 5.5
  • Sample 3: n=16, Mean(pg/ml): 2755, Standard deviation: 159.8, CV(%): 5.8,
  • Sample 1: n=24, Mean(pg/ml): 950, Standard deviation: 51.3, CV(%): 5.4
  • Sample 2: n=24, Mean(pg/ml): 2160, Standard deviation: 138.2, CV(%): 6.4
  • Sample 3: n=24, Mean(pg/ml): 3052, Standard deviation: 228.9, CV(%): 7.5
Restrictions For Research Use only

Handling

Product Details TNFRSF11A ELISA Kit Target details Application Details References for TNFRSF11A Kit (ABIN1889423) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for TNFRSF11A Kit (ABIN1889423)

Product Details TNFRSF11A ELISA Kit Target details Application Details Handling Images back to top
Background publications

Darnay, Haridas, Ni, Moore, Aggarwal et al.: "Characterization of the intracellular domain of receptor activator of NF-kappaB (RANK). Interaction with tumor necrosis factor receptor-associated factors and activation of NF-kappab and c-Jun ..." in: The Journal of biological chemistry, Vol. 273, Issue 32, pp. 20551-5, 1998

Images

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Supplier Images
ELISA image for TNFRSF11A ELISA Kit (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator) (ABIN1889423) Rat RANK PicoKine ELISA Kit standard curve