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anti-Cow (Bovine) Tumor Necrosis Factor antibody for Western Blotting

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Epitope
AA 1-234
37
30
28
25
23
23
18
16
13
12
10
10
10
6
3
3
3
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Cow (Bovine)
1242
465
346
144
138
123
123
56
55
45
31
18
8
8
7
7
4
4
4
3
3
2
2
1
1
1
Host
Rabbit
960
479
213
43
13
9
9
8
4
1
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
173
99
71
49
35
31
29
27
27
26
26
26
26
26
26
25
25
25
20
9
7
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
882
804
582
257
255
166
146
145
136
123
104
63
25
25
24
22
22
19
15
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1860792
$ 369.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN2476869 WB Mouse IgG1 Log in to see VPM62 2
0.0011728023 ABIN1582141 IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal 5
0.0011728023 ABIN1582139 IP IHC ELISA WB Mouse IgG Log in to see 2
0.0011728023 ABIN121801 EIA WB Biotin Rabbit Log in to see Polyclonal
0.0011728023 ABIN958283 ICC ELISA WB Goat IgG Log in to see Polyclonal
0.0011728023 ABIN121800 EIA WB Rabbit Log in to see Polyclonal
0.0011728023 ABIN212938 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN1046140 ELISA WB Mouse IgG2b Log in to see
0.0011728023 ABIN217583 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN2476870 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 2
0.0011728023 ABIN2476871 ELISA WB Rabbit IgG Log in to see Polyclonal 2
0.0011728023 ABIN2497032 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN2497033 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN1046204 IHC ELISA WB FITC Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN1046205 IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN1046203 IHC ELISA WB HRP Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN599625 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN615650 IHC (fro) RIA WB Rabbit Log in to see Polyclonal
0.0011728023 ABIN2380235 ELISA WB Biotin Rabbit Log in to see Polyclonal
0.0011728023 ABIN1046202 IHC ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-Tumor Necrosis Factor antibody for Western Blotting

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Cow (Bovine)
ELISA 42 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 11 Antibodies
Functional Studies (Func) 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunohistochemistry (IHC) 15 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Western Blotting (WB) 36 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 1-234
(37), (30), (28), (25), (23), (23), (18), (16), (13), (12), (10), (10), (10), (6), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine)
(1242), (465), (346), (144), (138), (123), (123), (56), (55), (45), (31), (18), (8), (8), (7), (7), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1)
Host Rabbit
(960), (479), (213), (43), (13), (9), (9), (8), (4), (1)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(173), (99), (71), (49), (35), (31), (29), (27), (27), (26), (26), (26), (26), (26), (26), (25), (25), (25), (20), (9), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(882), (804), (582), (257), (255), (166), (146), (145), (136), (123), (104), (63), (25), (25), (24), (22), (22), (19), (15), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MSTKSMIRDV ELAEEVLSEK AGGPQGSRSC LCLSLFSFLL VAGATTLFCL LHFGVIGPQR EEQSPGGPSI NSPLVQTLRS SSQASSNKPV AHVVADINSP GQLRWWDSYA NALMANGVKL EDNQLVVPAD GLYLIYSQVL FRGQGCPSTP LFLTHTISRI AVSYQTKVNI LSAIKSPCHR ETPEWAEAKP WYEPIYQGGV FQLEKGDRLS AEINLPDYLD YAESGQVYFG IIAL
Specificity It has been selected for its ability to recognize TNFa in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen TNFa (AA 1-234)
Isotype IgG

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name Tumor Necrosis Factor Alpha (TNFa) (TNF Antibody Abstract)
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant TNFa (Met1~Leu234) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Tumor Necrosis Factor antibody (TNF) (AA 1-234) (ABIN1860792) anti-Tumor Necrosis Factor (TNF) (AA 1-234) antibody