anti-Human Tumor Necrosis Factor antibody for Enzyme Immunoassay

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Epitope
AA 115-130
51
44
29
27
24
22
17
16
15
11
11
10
10
8
4
4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
1250
461
359
145
136
119
119
63
55
41
29
18
14
12
9
7
6
4
4
4
4
2
1
1
1
1
Host
Mouse
934
519
184
48
10
9
7
6
4
3
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
169
100
74
50
32
30
28
27
26
25
25
25
25
25
24
24
24
23
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
887
858
621
320
287
230
219
190
138
117
97
68
29
29
26
22
20
18
14
9
7
7
6
6
6
5
5
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN118949
$ 467.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN115771 EIA Func IHC (p) WB Mouse IgG1 Log in to see 2
1 ABIN1109390 EIA WB Mouse IgG1 Log in to see J2D10 1
1 ABIN1109391 EIA WB Mouse IgG1 Log in to see J1D9 1
1 ABIN781720 ELISA (Capture) Func EIA Mouse IgG1 Log in to see 2C8 4
1 ABIN782382 EIA IHC (p) WB Mouse IgG1 AA 1-234 Log in to see M1-C4
1 ABIN126020 EIA FACS IHC (fro) Rat IgG1 Log in to see MP9-20A4 2
1 ABIN614424 Func EIA WB Mouse IgG1 Log in to see MAb1
1 ABIN1109383 Func EIA IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see 52B83 4
1 ABIN1109384 Func EIA IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see 4H31 1
1 ABIN452504 EIA WB Mouse IgG1 Log in to see B1E4
1 ABIN126018 EIA Func FACS IHC (fro) Rat IgG1 Log in to see MP9-20A4 2
1 ABIN264362 EIA Biotin Mouse IgG1 Log in to see AS1
1 ABIN1109404 EIA WB Biotin Mouse IgG1 Log in to see T3 1
1 ABIN1109405 EIA WB Biotin Mouse IgG1 Log in to see T1 1
1 ABIN1109407 EIA WB FITC Mouse IgG1 Log in to see T1 1
1 ABIN452502 EIA Biotin Mouse IgG1 Log in to see B1E4
1 ABIN5556640 EIA Func IHC (fro) IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN5556639 EIA Func IHC (fro) IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN181044 EIA FACS Rat IgG1 Log in to see GMO1-17A2 1
1 ABIN1109385 EIA Mouse Log in to see 3-5F2 1

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 115-130
(51), (44), (29), (27), (24), (22), (17), (16), (15), (11), (11), (10), (10), (8), (4), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1250), (461), (359), (145), (136), (119), (119), (63), (55), (41), (29), (18), (14), (12), (9), (7), (6), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Host Mouse
(934), (519), (184), (48), (10), (9), (7), (6), (4), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(169), (100), (74), (50), (32), (30), (28), (27), (26), (25), (25), (25), (25), (25), (24), (24), (24), (23), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(887), (858), (621), (320), (287), (230), (219), (190), (138), (117), (97), (68), (29), (29), (26), (22), (20), (18), (14), (9), (7), (7), (6), (6), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN118949) Images
Purification Supernatant
Immunogen 115-130 sequence linked to thyroglobulin. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag14 myeloma cell line.
Clone 4C6-H6
Isotype IgM

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN118949) Images back to top
Antigen
Alternative Name Tumor Necrosis Factor (TNF-Alpha) (TNF Antibody Abstract)
Background Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
Gene ID 9606
UniProt P01375
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN118949) Images back to top
Application Notes Flow Cytometry: Membrane permeabilisation is required for this application. ELISA: 1/2 - 1/10. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN118949) Images back to top
Format Liquid
Buffer 0.2 M Tris/HCl pH 7.4 containing 5-10 % Foetal Calf Serum, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN118949)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Background publications

Bebök, Márkus, Németh: "Prognostic relevance of transforming growth factor alpha (TGF-alpha) and tumor necrosis factor alpha (TNF-alpha) detected in breast cancer tissues by immunohistochemistry." in: Breast cancer research and treatment, Vol. 29, Issue 3, pp. 229-35, 1994