anti-Human Tumor Necrosis Factor antibody for ELISA (Capture)

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Reactivity
Human
1258
465
359
145
136
119
119
63
55
41
29
18
14
12
9
7
6
4
4
4
4
2
1
1
1
1
Host
Mouse
941
521
186
49
10
9
7
6
4
3
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
169
100
78
50
32
30
28
27
26
25
25
25
25
25
24
24
24
23
13
9
8
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
ELISA (Capture), Functional Studies (Func), Enzyme Immunoassay (EIA)
888
860
633
323
287
230
221
191
139
118
96
68
30
29
26
22
20
18
14
9
7
7
6
6
6
6
5
5
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN781720
$ 346.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1109381 ELISA (Capture) ELISpot Mouse IgG1 Log in to see B-F7
1 ABIN191333 ELISA (Capture) Func IHC ELISA Mouse IgG1 Log in to see 2C8
1 ABIN5540917 ELISA (Capture) EIA Func Mouse IgG1 Log in to see Mab1
1 ABIN5589760 ELISA ELISA (Capture) IA IHC (p) Mouse IgG1 kappa Log in to see CH8820
1 ABIN1722433 ELISA (Capture) ELISPOT (Capture) Mouse IgG1 Log in to see B-F7

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human
(1258), (465), (359), (145), (136), (119), (119), (63), (55), (41), (29), (18), (14), (12), (9), (7), (6), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Host Mouse
(941), (521), (186), (49), (10), (9), (7), (6), (4), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(169), (100), (78), (50), (32), (30), (28), (27), (26), (25), (25), (25), (25), (25), (24), (24), (24), (23), (13), (9), (8), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application ELISA (Capture), Functional Studies (Func), Enzyme Immunoassay (EIA)
(888), (860), (633), (323), (287), (230), (221), (191), (139), (118), (96), (68), (30), (29), (26), (22), (20), (18), (14), (9), (7), (7), (6), (6), (6), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN781720) Images
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity Chromatography, Endotoxin depleted
Immunogen Recombinant Human TNF alpha
Clone 2C8
Isotype IgG1

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN781720) Images back to top
Antigen
Alternative Name Tumor Necrosis Factor (TNF-Alpha) (TNF Antibody Abstract)
Background Tumor necrosis factor-alpha (TNF-alpha), a homotrimeric 17 KD protein, is a potent mediator of inflammatory and metabolic functions. TNF-alpha was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Further TNF-alpha has been implied as central mediator in shock induced by gram negative micro-organisms. The cytokine TNF-alpha is found to be a central mediator in many inflammatory and immunological processes: it can be induced by various products of micro-organisms and by various cytokines but it also induces on its turn the production of many cytokines. Signal transduction occurs via two types of TNF-receptors, the TNF-receptors I and II. The receptors differ strongly in their intra-cellular signaling pathways. The TNF-alpha trimer interacts with either of the two types of TNF-R leading to receptor cross-linking.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
Gene ID 7124
NCBI Accession NP_000585
UniProt P01375
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor antibody (ABIN781720) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor antibody (ABIN781720) Images back to top
Reconstitution Restore with 0.5 mL distilled water.
Concentration 0.2 mg/mL
Buffer PBS, pH 7.2, None, None
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store (in aliquots) at -20 °C.

References for anti-Tumor Necrosis Factor antibody (ABIN781720)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Background publications

Tartaglia, Weber, Figari, Reynolds, Palladino, Goeddel: "The two different receptors for tumor necrosis factor mediate distinct cellular responses." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 20, pp. 9292-6, 1991

Old: "Tumour necrosis factor. Polypeptide mediator network." in: Nature, Vol. 326, Issue 6111, pp. 330-1, 1987

Smith, Baglioni: "The active form of tumor necrosis factor is a trimer." in: The Journal of biological chemistry, Vol. 262, Issue 15, pp. 6951-4, 1987

Kramer, Carver: "Serum-free in vitro bioassay for the detection of tumor necrosis factor." in: Journal of immunological methods, Vol. 93, Issue 2, pp. 201-6, 1986