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anti-Human Tumor Necrosis Factor antibody for ELISA (Capture)

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Human
1232
462
344
144
138
123
123
56
52
45
31
18
8
7
5
4
4
4
3
3
3
2
2
1
1
1
Host
Mouse
952
479
210
43
13
9
9
7
4
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
172
98
71
49
35
31
29
27
27
26
26
26
26
26
26
25
25
25
20
9
7
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
ELISA (Capture), Functional Studies (Func), Enzyme Immunoassay (EIA)
880
797
577
252
249
163
145
142
135
121
102
62
25
25
23
22
22
17
15
8
7
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN781720
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008696448 ABIN1109381 ELISA (Capture) ELISpot Mouse IgG1 Log in to see B-F7
0.0008696448 ABIN191333 ELISA (Capture) Func IHC ELISA Mouse IgG1 Log in to see 2C8
0.0008696448 ABIN282564 ELISA (Capture) ELISPOT (Capture) Neut Mouse IgG1 kappa Log in to see MAb1
0.0008696448 ABIN281695 ELISA (Capture) ELISPOT (Capture) Mouse IgG1 kappa Log in to see MAb1
0.0008696448 ABIN1722433 ELISA (Capture) ELISPOT (Capture) Mouse IgG1 Log in to see B-F7

Top referenced anti-Tumor Necrosis Factor antibody for ELISA (Capture)

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 13 Antibodies
Blocking Reagent (BR) 2 Antibodies
Cellular Assay (CA) 2 Antibodies
Dot Blot (DB) 4 Antibodies
ELISA 500 Antibodies
ELISA (Capture) 6 Antibodies
ELISA (Detection) 4 Antibodies
ELISpot 9 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 3 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 2 Antibodies
Enzyme Immunoassay (EIA) 42 Antibodies
Flow Cytometry (FACS) 473 Antibodies
Functional Studies (Func) 72 Antibodies
Immunoassay (IA) 16 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 96 Antibodies
Immunofluorescence (IF) 136 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human
(1232), (462), (344), (144), (138), (123), (123), (56), (52), (45), (31), (18), (8), (7), (5), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(952), (479), (210), (43), (13), (9), (9), (7), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(172), (98), (71), (49), (35), (31), (29), (27), (27), (26), (26), (26), (26), (26), (26), (25), (25), (25), (20), (9), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application ELISA (Capture), Functional Studies (Func), Enzyme Immunoassay (EIA)
(880), (797), (577), (252), (249), (163), (145), (142), (135), (121), (102), (62), (25), (25), (23), (22), (22), (17), (15), (8), (7), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN781720) Images
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity Chromatography, Endotoxin depleted
Immunogen Recombinant Human TNF alpha
Clone 2C8
Isotype IgG1

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN781720) Images back to top
Antigen
Alternative Name Tumor Necrosis Factor (TNF-Alpha) (TNF Antibody Abstract)
Background Tumor necrosis factor-alpha (TNF-alpha), a homotrimeric 17 KD protein, is a potent mediator of inflammatory and metabolic functions. TNF-alpha was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Further TNF-alpha has been implied as central mediator in shock induced by gram negative micro-organisms. The cytokine TNF-alpha is found to be a central mediator in many inflammatory and immunological processes: it can be induced by various products of micro-organisms and by various cytokines but it also induces on its turn the production of many cytokines. Signal transduction occurs via two types of TNF-receptors, the TNF-receptors I and II. The receptors differ strongly in their intra-cellular signaling pathways. The TNF-alpha trimer interacts with either of the two types of TNF-R leading to receptor cross-linking.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
Gene ID 7124
NCBI Accession NP_000585
UniProt P01375
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN781720) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN781720) Images back to top
Reconstitution Restore with 0.5 mL distilled water.
Concentration 0.2 mg/mL
Buffer PBS, pH 7.2, None, None
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store (in aliquots) at -20 °C.

References for anti-Tumor Necrosis Factor Antibody (ABIN781720)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Background publications

Tartaglia, Weber, Figari, Reynolds, Palladino, Goeddel: "The two different receptors for tumor necrosis factor mediate distinct cellular responses." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 20, pp. 9292-6, 1991

Old: "Tumour necrosis factor. Polypeptide mediator network." in: Nature, Vol. 326, Issue 6111, pp. 330-1, 1987

Smith, Baglioni: "The active form of tumor necrosis factor is a trimer." in: The Journal of biological chemistry, Vol. 262, Issue 15, pp. 6951-4, 1987

Kramer, Carver: "Serum-free in vitro bioassay for the detection of tumor necrosis factor." in: Journal of immunological methods, Vol. 93, Issue 2, pp. 201-6, 1986