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anti-Human Tumor Necrosis Factor antibody for Inhibition Assay

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Human
1199
442
329
139
132
118
118
55
55
31
26
18
8
8
7
7
4
4
4
3
2
2
2
1
1
1
Host
Mouse
914
477
201
44
13
9
7
4
3
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
167
95
69
49
32
30
27
26
25
25
25
25
25
25
24
24
24
18
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunoassay (IA), Inhibition Assay (InhA)
853
797
536
253
251
163
141
140
134
124
101
63
25
24
23
22
17
14
8
6
6
5
5
5
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2192066
$ 382.63
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000870507 ABIN2191890 IA FACS InhA IP WB Mouse Log in to see T1 1

Top referenced anti-Tumor Necrosis Factor antibody for Inhibition Assay

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 13 Antibodies
Blocking Peptide (BP) 20 Antibodies
Blocking Reagent (BR) 2 Antibodies
Cellular Assay (CA) 2 Antibodies
Dot Blot (DB) 4 Antibodies
ELISA 498 Antibodies
ELISA (Capture) 4 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot 9 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 42 Antibodies
Flow Cytometry (FACS) 437 Antibodies
Functional Studies (Func) 71 Antibodies
Immunoassay (IA) 15 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 95 Antibodies
Immunofluorescence (IF) 132 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human
(1199), (442), (329), (139), (132), (118), (118), (55), (55), (31), (26), (18), (8), (8), (7), (7), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(914), (477), (201), (44), (13), (9), (7), (4), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(167), (95), (69), (49), (32), (30), (27), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (18), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunoassay (IA), Inhibition Assay (InhA)
(853), (797), (536), (253), (251), (163), (141), (140), (134), (124), (101), (63), (25), (24), (23), (22), (17), (14), (8), (6), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2192066) Images
Sterility 0.2 μm filtered
Clone 5N

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2192066) Images back to top
Antigen
Alternative Name Tumor Necrosis Factor alpha (TNF Antibody Abstract)
Background The monoclonal antibody 5N reacts with Tumor Necrosis Factor alpha (TNF-alpha). Tumor necrosis factor-alpha (TNF-alpha), a homotrimeric 17 KD protein, is a potent mediator of inflammatory and metabolic functions. TNF-alpha was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Further TNF-alpha has been implied as central mediator in shock induced by gram negative micro-organisms. The cytokine TNF-alpha is found to be a central mediator in many inflammatory and immunological processes: it can be induced by various products of micro-organisms and by various cytokines but it also induces on its turn the production of many cytokines. Signal transduction occurs via two types of TNF-receptors, the TNF-receptors I and II. The receptors differ strongly in their intra-cellular signaling pathways. The TNF-alpha trimer interacts with either of the two types of TNF-R leading to receptor cross-linking. The monoclonal antibody 5N cross-reacts and cross-neutralizes all monkey species TNF-alpha, except pig-tail macaque. With affinity about two orders lower it recognizes bovine and canine TNF-alpha. Aliases TNF, TNF-SF2, DIF, Cachectin
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2192066) Images back to top
Application Notes For neutralization of biological activity in vitro dilutions have to be made according to the amounts of -10 TNF-alpha to be inactivated. The affinity of the monoclonal antibody 5N is 5.7 x 10 M. The homologous ELISA is useful for the detection of oligomeric human TNF-alpha. It does not recognize monomeric TNF-alpha.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN2192066) Images back to top
Buffer PBS, containing 0.1 % bovine serum albumin.
Storage 4 °C
Storage Comment Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Expiry Date 12 months

References for anti-Tumor Necrosis Factor Antibody (ABIN2192066)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Song, Fox, Gallo, Rosenberg, Jordan, Shealy, Wagner: "Effects of 2 different anti-tumor necrosis factor-alpha agents in a primate model of subcutaneous abscess formation." in: The Journal of infectious diseases, Vol. 185, Issue 2, pp. 204-13, 2002

Petyovka, Lyach, Voitenok: "Homologous ELISA for detection of oligomeric human TNF: properties of the assay." in: Journal of immunological methods, Vol. 186, Issue 2, pp. 161-70, 1995