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anti-Human Tumor Necrosis Factor antibody for Inhibition Assay

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Human
706
308
142
55
52
31
31
27
23
18
12
12
8
7
5
4
4
4
3
3
2
2
1
1
1
Host
Mouse
539
394
157
43
13
9
9
7
4
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
147
83
44
24
20
9
9
7
5
4
4
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunoassay (IA), Flow Cytometry (FACS), Inhibition Assay (InhA), Immunoprecipitation (IP), Western Blotting (WB)
635
588
318
208
120
100
99
85
80
63
56
56
25
25
22
22
15
13
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2191890
$ 421.58
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008676636 ABIN2192066 IA InhA Mouse Log in to see 5N 2

Top referenced anti-Tumor Necrosis Factor antibody for Inhibition Assay

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 13 Antibodies
Blocking Reagent (BR) 2 Antibodies
Cellular Assay (CA) 2 Antibodies
Dot Blot (DB) 4 Antibodies
ELISA 343 Antibodies
ELISA (Capture) 6 Antibodies
ELISA (Detection) 4 Antibodies
ELISpot 9 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 3 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 2 Antibodies
Enzyme Immunoassay (EIA) 42 Antibodies
Flow Cytometry (FACS) 217 Antibodies
Functional Studies (Func) 68 Antibodies
Immunoassay (IA) 13 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 31 Antibodies
Immunofluorescence (IF) 50 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human
(706), (308), (142), (55), (52), (31), (31), (27), (23), (18), (12), (12), (8), (7), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(539), (394), (157), (43), (13), (9), (9), (7), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(147), (83), (44), (24), (20), (9), (9), (7), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunoassay (IA), Flow Cytometry (FACS), Inhibition Assay (InhA), Immunoprecipitation (IP), Western Blotting (WB)
(635), (588), (318), (208), (120), (100), (99), (85), (80), (63), (56), (56), (25), (25), (22), (22), (15), (13), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2191890) Images
Sterility 0.2 μm filtered
Clone T1

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2191890) Images back to top
Antigen
Alternative Name Tumor Necrosis Factor alpha (TNF Antibody Abstract)
Background The antibody reacts with free soluble (17 kDa) and membrane (26 kDa) human TNF-alpha. The antibody inhibits the biological activity of both forms. It does not react with receptor bound TNF-alpha. It can be a useful tool to discriminate between the membrane form of TNF expressed on producer cells and the proteolytically cleaved, soluble TNF-alpha bound to its cognate cell membrane receptors (TNF-RI and TNF-RII). For this purpose we recommend to use this antibody in combination with the anti-TNF-alpha antibody HM2026, which recognizes soluble, membrane and receptor bound TNF-alpha.
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2191890) Images back to top
Application Notes For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For neutralization of biological activity in vitro dilutions have to be made according to the amounts of TNF-alpha to be inactivated.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN2191890) Images back to top
Buffer PBS, containing 0.1 % bovine serum albumin.
Storage 4 °C
Storage Comment Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Expiry Date 12 months

References for anti-Tumor Necrosis Factor Antibody (ABIN2191890)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Gerspach, Götz, Zimmermann, Kolle, Böttinger, Grell: "Detection of membrane-bound tumor necrosis factor (TNF): an analysis of TNF-specific reagents." in: Microscopy research and technique, Vol. 50, Issue 3, pp. 243-50, 2000