anti-Human Tumor Necrosis Factor antibody for Blocking Antibody

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Reactivity
Human
1258
465
359
145
136
119
119
63
55
41
29
18
14
12
9
7
6
4
4
4
4
2
1
1
1
1
Host
Mouse
941
521
186
49
10
9
7
6
4
3
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
169
100
78
50
32
30
28
27
26
25
25
25
25
25
24
24
24
23
13
9
8
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Blocking Antibody (Inhibition), Flow Cytometry (FACS)
888
860
632
323
287
230
221
191
139
118
97
69
30
29
26
21
20
18
14
9
8
7
6
6
6
6
5
5
3
3
3
2
2
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN2689932
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN636697 Inhibition ELISA WB Rabbit Log in to see Polyclonal 1
1 ABIN345761 Inhibition IHC Mouse IgG1 Log in to see 1D9
1 ABIN2191892 Inhibition IA FACS IP WB Biotin Mouse Log in to see T 1 1
1 ABIN1003395 Inhibition ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1003396 Inhibition ELISA WB Goat IgG Log in to see Polyclonal
1 ABIN284779 Inhibition ELISA WB Mouse IgG1 kappa Log in to see M26
1 ABIN289547 Inhibition ELISA WB Goat IgG1 kappa Log in to see Polyclonal
1 ABIN284915 Inhibition IHC ELISA Mouse IgG1 Log in to see M32253
1 ABIN284821 Inhibition IHC ELISA Mouse IgG1 Log in to see 1D9
1 ABIN284914 Inhibition IHC ELISA Mouse IgG1 Log in to see M32254
1 ABIN284820 Inhibition IHC ELISA Mouse IgG1 Log in to see 2C8
1 ABIN285975 Inhibition ELISA WB Goat Log in to see Polyclonal

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human
(1258), (465), (359), (145), (136), (119), (119), (63), (55), (41), (29), (18), (14), (12), (9), (7), (6), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Host Mouse
(941), (521), (186), (49), (10), (9), (7), (6), (4), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(169), (100), (78), (50), (32), (30), (28), (27), (26), (25), (25), (25), (25), (25), (24), (24), (24), (23), (13), (9), (8), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Blocking Antibody (Inhibition), Flow Cytometry (FACS)
(888), (860), (632), (323), (287), (230), (221), (191), (139), (118), (97), (69), (30), (29), (26), (21), (20), (18), (14), (9), (8), (7), (6), (6), (6), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN2689932) Images
Brand BD Pharmingen™
Characteristics The MAb11 antibody reacts with human tumor necrosis factor (TNF, formerly known as TNF-α) protein. TNF is an efficient paracrine and endocrine mediator of inflammatory and immune functions. It regulates the growth and differentiation of a variety of cell types. TNF is cytotoxic for transformed cells when in conjunction with IFN-γ. It is secreted by activated monocytes/macrophages and other cells such as B cells, T cells and fibroblasts. The immunogen used to generate the MAb11 hybridoma was recombinant human TNF. The use of the MAb11 antibody has been reported to cross-react with TNF of rhesus monkey. This antibody is routinely tested by intracellular staining. Other applications were tested during antibody development only or reported in the literature. Expression of TNF by stimulated human peripheral blood mononuclear cells (PBMC). Human PBMC were stimulated for 6 hours with PMA (Sigma, Cat. #P-8139) and calcium ionophore A23187 (Sigma, Cat. #C-9275) in the presence of GolgiStop™ (2 μM final concentration, Cat. No. 554724). The PBMC were stained with PE-Cy5 anti-CD3 (PE-Cy5 UCHT1, Cat. No. 555334), fixed, permeabilized, and subsequently stained with 0.25 μg of FITC-conjugated mouse anti-human TNF antibody (FITC-MAb11, Cat. No. 554512) by using the BD Pharmingen staining protocol (left panel). To demonstrate specificity of staining, the binding of FITC MAb11 was blocked by the preincubation of the fluorochrome-conjugated antibody with recombinant human TNF (5 μg, Cat. No. 554618, middle panel), and by preincubation of the fixed/permeabilized cells with unlabeled MAb11 antibody (5 μg, Cat. No. 554510, right panel) prior to staining with the FITC-MAb11 antibody. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking and unlabeled antibody blocking specificity controls.

BD Pharmingen™ Purified Mouse Anti-Human TNF - Purified - Clone MAb11 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Recombinant Human TNF
Clone MAb11
Isotype IgG1, kappa

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN2689932) Images back to top
Antigen
Alternative Name TNF (TNF Antibody Abstract)
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor antibody (ABIN2689932) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor antibody (ABIN2689932) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-Tumor Necrosis Factor antibody (ABIN2689932)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996

Rathjen, Cowan, Furphy, Aston: "Antigenic structure of human tumour necrosis factor: recognition of distinct regions of TNF alpha by different tumour cell receptors." in: Molecular immunology, Vol. 28, Issue 1-2, pp. 79-86, 1991