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anti-Mouse (Murine) Tumor Necrosis Factor antibody for Biochemical Assay

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Mouse (Murine)
1233
461
344
144
138
123
123
56
52
45
31
18
8
7
5
4
4
4
3
3
3
2
2
1
1
1
Host
Rat
953
479
209
43
13
9
9
7
4
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is conjugated to PerCP-Cy5.5
172
98
71
49
35
31
29
27
27
26
26
26
26
26
26
25
25
25
20
9
7
4
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Biochemical Assay (BCA)
880
797
577
252
249
163
145
142
135
121
103
63
25
25
23
22
22
17
15
8
8
6
6
5
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2660471
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008862732 ABIN2660472 BCA PerCP-Cy5.5 Rat IgG1 kappa Log in to see MP6-XT22 11

Top referenced anti-Tumor Necrosis Factor antibody for Biochemical Assay

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 2 Antibodies
Blocking Antibody (Inhibition) 7 Antibodies
Blocking Reagent (BR) 3 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 156 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
ELISpot 4 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 152 Antibodies
Functional Studies (Func) 19 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 69 Antibodies
Immunofluorescence (IF) 85 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 69 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Mouse (Murine)
(1233), (461), (344), (144), (138), (123), (123), (56), (52), (45), (31), (18), (8), (7), (5), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1)
Host Rat
(953), (479), (209), (43), (13), (9), (9), (7), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is conjugated to PerCP-Cy5.5
(172), (98), (71), (49), (35), (31), (29), (27), (27), (26), (26), (26), (26), (26), (26), (25), (25), (25), (20), (9), (7), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Biochemical Assay (BCA)
(880), (797), (577), (252), (249), (163), (145), (142), (135), (121), (103), (63), (25), (25), (23), (22), (22), (17), (15), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 11 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2660471) Images
Purification The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Clone MP6-XT22
Isotype IgG1 kappa

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2660471) Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is a 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD. TNF-α forms multimeric complexes, stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
Research Area Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2660471) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN2660471) Images back to top
Concentration 0.2 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-Tumor Necrosis Factor Antibody (ABIN2660471)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Background publications

Via, Shustov, Rus, Lang, Nguyen, Finkelman: "In vivo neutralization of TNF-alpha promotes humoral autoimmunity by preventing the induction of CTL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 12, pp. 6821-6, 2001

Infante-Duarte, Horton, Byrne, Kamradt: "Microbial lipopeptides induce the production of IL-17 in Th cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 11, pp. 6107-15, 2000

Jacobs, Brown, Allie, Gulert, Ryffel: "Increased resistance to mycobacterial infection in the absence of interleukin-10." in: Immunology, Vol. 100, Issue 4, pp. 494-501, 2000

Williams, Marinova-Mutafchieva, Feldmann, Maini: "Evaluation of TNF-alpha and IL-1 blockade in collagen-induced arthritis and comparison with combined anti-TNF-alpha/anti-CD4 therapy." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 12, pp. 7240-5, 2000

Marinova-Mutafchieva, Williams, Mason, Mauri, Feldmann, Maini: "Dynamics of proinflammatory cytokine expression in the joints of mice with collagen-induced arthritis (CIA)." in: Clinical and experimental immunology, Vol. 107, Issue 3, pp. 507-12, 1997

Mo, Sarawar, Doherty: "Induction of cytokines in mice with parainfluenza pneumonia." in: Journal of virology, Vol. 69, Issue 2, pp. 1288-91, 1995

Sarawar, Sangster, Coffman, Doherty: "Administration of anti-IFN-gamma antibody to beta 2-microglobulin-deficient mice delays influenza virus clearance but does not switch the response to a T helper cell 2 phenotype." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 3, pp. 1246-53, 1994

Tracey, Cerami: "Tumor necrosis factor: an updated review of its biology." in: Critical care medicine, Vol. 21, Issue 10 Suppl, pp. S415-22, 1993

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992

Beutler, Cerami: "The biology of cachectin/TNF--a primary mediator of the host response." in: Annual review of immunology, Vol. 7, pp. 625-55, 1989

Beutler, Cerami: "Tumor necrosis, cachexia, shock, and inflammation: a common mediator." in: Annual review of biochemistry, Vol. 57, pp. 505-18, 1988

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2660471) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor antibody (TNF)  (PerCP-Cy5.5) (ABIN2660471) anti-Tumor Necrosis Factor (TNF) antibody (PerCP-Cy5.5)
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor antibody (TNF)  (PerCP-Cy5.5) (ABIN2660471) anti-Tumor Necrosis Factor (TNF) antibody (PerCP-Cy5.5) (Image 2)