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anti-Mouse (Murine) Tumor Necrosis Factor antibody for ELISA (Capture)

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Epitope
AA 80-235
44
38
27
26
22
21
17
17
15
11
11
10
10
8
4
4
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
1216
442
357
142
135
119
119
56
52
41
28
17
11
9
9
7
6
4
4
4
4
2
1
1
1
Host
Goat
924
482
182
41
10
9
7
6
3
2
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
160
95
70
49
32
30
27
26
25
25
25
25
25
25
24
24
24
19
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), Western Blotting (WB)
865
838
591
307
273
229
201
179
136
119
97
63
27
26
25
22
20
16
14
8
7
6
6
6
5
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN4900489
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Top referenced anti-Tumor Necrosis Factor antibody for ELISA (Capture)

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 2 Antibodies
Blocking Antibody (Inhibition) 7 Antibodies
Blocking Peptide (BP) 3 Antibodies
Blocking Reagent (BR) 2 Antibodies
Cell Culture (CC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 10 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 196 Antibodies
ELISA (Capture) 1 Antibodies
ELISpot 3 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 190 Antibodies
Functional Studies (Func) 20 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 113 Antibodies
Immunofluorescence (IF) 128 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 80-235
(44), (38), (27), (26), (22), (21), (17), (17), (15), (11), (11), (10), (10), (8), (4), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(1216), (442), (357), (142), (135), (119), (119), (56), (52), (41), (28), (17), (11), (9), (9), (7), (6), (4), (4), (4), (4), (2), (1), (1), (1)
Host Goat
(924), (482), (182), (41), (10), (9), (7), (6), (3), (2)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(160), (95), (70), (49), (32), (30), (27), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (19), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), Western Blotting (WB)
(865), (838), (591), (307), (273), (229), (201), (179), (136), (119), (97), (63), (27), (26), (25), (22), (20), (16), (14), (8), (7), (6), (6), (6), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 20 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN4900489) Images
Specificity Detects mouse TNF-alpha in ELISAs and Western blots. In sandwich immunoassays, approximately 50 % cross-reactivity with recombinant rat TNF-alpha is observed and less than 1 % cross-reactivity with recombinant human TNF-alpha is observed.
Purification Immunogen affinity purified
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen E. coli-derived recombinant mouse TNF-alpha, Leu80-Leu235, Accession # P06804
Isotype IgG

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN4900489) Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background Alternative Names: APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, tnfa, TNF-a, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
Gene ID 7124
UniProt P06804
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN4900489) Images back to top
Application Notes Western Blot 0.1 μg/mL
Neutralization 0.1-0.4 μg/mL
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 5-15 μg/mL
CyTOF-ready
ELISA Capture (Matched Antibody Pair) 0.2-0.8 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN4900489) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-Tumor Necrosis Factor Antibody (ABIN4900489)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Lim, Yuzhalin, Gordon-Weeks, Muschel: "Tumor-infiltrating monocytes/macrophages promote tumor invasion and migration by upregulating S100A8 and S100A9 expression in cancer cells." in: Oncogene, Vol. 35, Issue 44, pp. 5735-5745, 2016 Method employed by authors: Neutralization (Neut) (Sample species: Mouse (Murine)).

Ramkhelawon, Hennessy, Ménager, Ray, Sheedy, Hutchison, Wanschel, Oldebeken, Geoffrion, Spiro, Miller, McPherson, Rayner, Moore: "Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity." in: Nature medicine, Vol. 20, Issue 4, pp. 377-84, 2014 Method employed by authors: Biochemical Assay (BCA) (Sample species: Mouse (Murine)).

Shikata, Sakaue, Nakashiro, Okazaki, Kurata, Okamura, Okura, Ryugo, Nakamura, Yasugi, Higashiyama, Izutani: "Pathophysiology of lung injury induced by common bile duct ligation in mice." in: PLoS ONE, Vol. 9, Issue 4, pp. e94550, 2014 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Ilett, Kottke, Donnelly, Thompson, Willmon, Diaz, Zaidi, Coffey, Selby, Harrington, Pandha, Melcher, Vile: "Cytokine conditioning enhances systemic delivery and therapy of an oncolytic virus." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 22, Issue 10, pp. 1851-63, 2014 Method employed by authors: Neutralization (Neut) (Sample species: Mouse (Murine)).

Clatworthy, Harford, Mathews, Smith: "FcγRIIb inhibits immune complex-induced VEGF-A production and intranodal lymphangiogenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 50, pp. 17971-6, 2014 Method employed by authors: Neutralization (Neut) (Sample species: Mouse (Murine)).

Hillian, McMullen, Sebastian, Roychowdhury, Rowchowdhury, Kashyap, Schauer, Kirwan, Feldstein, Nagy: "Mice lacking C1q are protected from high fat diet-induced hepatic insulin resistance and impaired glucose homeostasis." in: The Journal of biological chemistry, Vol. 288, Issue 31, pp. 22565-75, 2013 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Li, Shi, Shu, Gao, Wu, Tang et al.: "Wingless-type mammary tumor virus integration site family, member 5A (Wnt5a) regulates human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein 120 (gp120)-induced expression of ..." in: The Journal of biological chemistry, Vol. 288, Issue 19, pp. 13610-9, 2013 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Kim, Feng, Ohkuri, Sauers, Cohn, Chai, Nelson, Bachmanov, Huang, Wang: "Defects in the peripheral taste structure and function in the MRL/lpr mouse model of autoimmune disease." in: PLoS ONE, Vol. 7, Issue 4, pp. e35588, 2012 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Zhang, Yin, Zhang, Sun, Yang, Zhang, Salzman, Wang, Liu, Vidyasagar, Zhang, Ju, Okunieff, Zhang: "Response patterns of cytokines/chemokines in two murine strains after irradiation." in: Cytokine, Vol. 58, Issue 2, pp. 169-77, 2012 Method employed by authors: Luminex Assay (LMNX) (Sample species: Mouse (Murine)).

Fisher, Chen, Skitzki, Muhitch, Zhou, Appenheimer, Vardam, Weis, Passanese, Wang, Gollnick, Dewhirst, Rose-John, Repasky, Baumann, Evans: "IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells." in: The Journal of clinical investigation, Vol. 121, Issue 10, pp. 3846-59, 2011 Method employed by authors: In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Wieland, van der Windt, Florquin, McKenzie, van der Poll: "ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis." in: Microbes and infection, Vol. 11, Issue 4, pp. 524-30, 2009 Method employed by authors: ELISA (ELISA) (Sample species: Mouse (Murine)).

Wu, Mehta, Chakrabarty, Booth, Duggan, Patel, Ballard, Coggeshall, Metcalf: "Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 9, pp. 5799-806, 2009 Method employed by authors: ELISA (ELISA) (Sample species: Mouse (Murine)).

Nold-Petry, Nold, Nielsen, Bustamante, Zepp, Storm, Hong, Kim, Dinarello: "Increased cytokine production in interleukin-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling." in: The Journal of biological chemistry, Vol. 284, Issue 38, pp. 25900-11, 2009 Method employed by authors: ELISA (ELISA) (Sample species: Mouse (Murine)).

Koronyo-Hamaoui, Ko, Koronyo, Azoulay, Seksenyan, Kunis, Pham, Bakhsheshian, Rogeri, Black, Farkas, Schwartz: "Attenuation of AD-like neuropathology by harnessing peripheral immune cells: local elevation of IL-10 and MMP-9." in: Journal of neurochemistry, Vol. 111, Issue 6, pp. 1409-24, 2009 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Baluk, Yao, Feng, Romano, Jung, Schreiter, Yan, Shealy, McDonald: "TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice." in: The Journal of clinical investigation, Vol. 119, Issue 10, pp. 2954-64, 2009 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Joly, Francke, Ulbricht, Beck, Seeliger, Hirrlinger, Hirrlinger, Lang, Zinkernagel, Odermatt, Samardzija, Reichenbach, Grimm, Remé: "Cooperative phagocytes: resident microglia and bone marrow immigrants remove dead photoreceptors in retinal lesions." in: The American journal of pathology, Vol. 174, Issue 6, pp. 2310-23, 2009 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Wendt, Schulten, Stichel, Lübbert: "Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model." in: Journal of neurochemistry, Vol. 110, Issue 6, pp. 1931-41, 2009 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Kim, Kim, Gim, Lee, Lee, Ko, Kim, Han, Chung: "Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 4, pp. 2553-61, 2008 Method employed by authors: ELISA (ELISA) (Sample species: Mouse (Murine)).

Wieland, van der Windt, Wiersinga, Florquin, van der Poll: "CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis." in: Immunology, Vol. 125, Issue 2, pp. 272-9, 2008 Method employed by authors: ELISA (ELISA) (Sample species: Mouse (Murine)).

Muranski, Boni, Antony, Cassard, Irvine, Kaiser, Paulos, Palmer, Touloukian, Ptak, Gattinoni, Wrzesinski, Hinrichs, Kerstann, Feigenbaum, Chan, Restifo: "Tumor-specific Th17-polarized cells eradicate large established melanoma." in: Blood, Vol. 112, Issue 2, pp. 362-73, 2008 Method employed by authors: ELISA (ELISA) (Sample species: Mouse (Murine)).

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN4900489) back to top
Supplier Images
Neutralization (Neut) image for anti-Tumor Necrosis Factor antibody (TNF) (AA 80-235) (ABIN4900489) Cytotoxicity Induced by TNF-alpha and Neutralization by Mouse TNF-alpha Antibody. Rec...
Immunocytochemistry (ICC) image for anti-Tumor Necrosis Factor antibody (TNF) (AA 80-235) (ABIN4900489) TNF-alpha in Mouse T Cells. TNF-alpha was detected in immersion fixed activated mouse...
Immunocytochemistry (ICC) image for anti-Tumor Necrosis Factor antibody (TNF) (AA 80-235) (ABIN4900489) TNF-alpha in RAW 264.7 Mouse Cell Line. TNF-alpha was detected in immersion fixed RAW...