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anti-Mouse (Murine) Tumor Necrosis Factor antibody for Radioimmunoassay

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Mouse (Murine)
1242
464
346
144
138
123
123
56
56
45
31
18
8
8
7
7
4
4
4
3
3
2
2
1
1
1
Host
Rabbit
960
479
213
43
13
9
9
8
4
1
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
173
99
71
49
35
31
29
27
27
26
26
26
26
26
26
25
25
25
20
9
7
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
882
805
582
257
255
166
146
145
135
124
103
62
25
24
24
22
22
19
15
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN118027
$ 1,435.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008709465 ABIN118025 EIA Func IHC (fro) IP RIA WB Rabbit Log in to see Polyclonal 2
0.0008709465 ABIN107955 IP IHC Neut RIA ELISA WB Rabbit IgG Log in to see Polyclonal
0.0008709465 ABIN107954 IP IHC Neut RIA ELISA WB Rabbit Log in to see Polyclonal
0.0008709465 ABIN470638 ICC IHC (fro) IP Neut RIA ELISA WB Rabbit IgG Log in to see Polyclonal
0.0008709465 ABIN799617 IP IHC RIA ELISA WB Rabbit Log in to see Polyclonal

Top referenced anti-Tumor Necrosis Factor antibody for Radioimmunoassay

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 2 Antibodies
Blocking Antibody (Inhibition) 7 Antibodies
Blocking Reagent (BR) 2 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 157 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
ELISpot 4 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 152 Antibodies
Functional Studies (Func) 19 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 69 Antibodies
Immunofluorescence (IF) 85 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 70 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Mouse (Murine)
(1242), (464), (346), (144), (138), (123), (123), (56), (56), (45), (31), (18), (8), (8), (7), (7), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1)
Host Rabbit
(960), (479), (213), (43), (13), (9), (9), (8), (4), (1)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(173), (99), (71), (49), (35), (31), (29), (27), (27), (26), (26), (26), (26), (26), (26), (25), (25), (25), (20), (9), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
(882), (805), (582), (257), (255), (166), (146), (145), (135), (124), (103), (62), (25), (24), (24), (22), (22), (19), (15), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN118027) Images
Cross-Reactivity (Details) Species reactivity (tested):Mouse.
Purification Selective salt precipitation
Immunogen Recombinant Mouse TNF-alpha produced in E.coli.

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN118027) Images back to top
Antigen
Alternative Name Tumor Necrosis Factor (TNF-Alpha) (TNF Antibody Abstract)
Background Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
Gene ID 10090
UniProt P06804
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN118027) Images back to top
Application Notes Western Blot: Use HRP Goat-anti-Rabbit IgG [H&L] (code R1364HRP) and TMB as asubstrate. A working dilution range of 1: 500 to 1: 2,000 is suggested for this application to detect TNFαfrom supernatants or lysates of 2 x 10^6 endotoxin-stimulated mouse peripheral bloodmononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) mouse serumplus 10 ng/mL E. coli LPS. Immunohistochemistry: A dilution range of 1: 400 is suggested for this immunoassay. Either paraffin fixation or cryofixation can be used for immunohistochemistry using adilution of 1: 200 for staining of TNFα.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN118027) Images back to top
Concentration 1.1 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 2-8 °C.

References for anti-Tumor Necrosis Factor Antibody (ABIN118027)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Background publications

Birkas Kovats, Palik, Faludi, Cseh: "[Possible connection between ghrelin, resistin and TNF-alpha levels and the metabolic syndrome caused by atypical antipsychotics]" in: Neuropsychopharmacologia Hungarica : a Magyar Pszichofarmakológiai Egyesület lapja = official journal of the Hungarian Association of Psychopharmacology, Vol. 7, Issue 3, pp. 132-9, 2006

Nedwin, Naylor, Sakaguchi, Smith, Jarrett-Nedwin, Pennica, Goeddel, Gray: "Human lymphotoxin and tumor necrosis factor genes: structure, homology and chromosomal localization." in: Nucleic acids research, Vol. 13, Issue 17, pp. 6361-73, 1985