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|Application / Reactivity||Pig (Porcine)||Non-Human Primate|
|ELISA||42 Antibodies||4 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Flow Cytometry (FACS)||15 Antibodies||4 Antibodies|
|Functional Studies (Func)||21 Antibodies||4 Antibodies|
|Immunocytochemistry (ICC)||21 Antibodies||4 Antibodies|
|Immunofluorescence (IF)||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||1 Antibodies|
|Immunohistochemistry (IHC)||15 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||3 Antibodies||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||8 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||5 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|Neutralization (Neut)||2 Antibodies|
|Western Blotting (WB)||28 Antibodies|
|Antigen||Tumor Necrosis Factor (TNF) Antibodies|
|Reactivity||Human, Non-Human Primate, Pig (Porcine) Alternatives|
|Conjugate||This Tumor Necrosis Factor antibody is conjugated to PE Alternatives|
Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), ELISA
|5 references available|
|Supplier||Log in to see|
Product Details anti-Tumor Necrosis Factor AntibodyTarget Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN1690740) Images
|Specificity||The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).|
|Characteristics||The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.|
|Immunogen||Recombinant human TNF-alpha|
Target Details Tumor Necrosis FactorProduct Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN1690740) Images back to top
|Alternative Name||TNF (TNF Antibody Abstract)|
|Background||TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.|
|Pathways||NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN1690740) Images back to top
The reagent is designed for Flow Cytometry analysis of human blood cells using 10 µL reagent / 100 µL of whole blood or 10^6 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.
Working concentrations should be determined by the investigator.
Cytokine Signaling (Human)
|Restrictions||For Research Use only|
HandlingProduct Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN1690740) Images back to top
|Reconstitution||No reconstitution is necessary.|
|Buffer||The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
Do not freeze.
Avoid prolonged exposure to light.
|Storage Comment||Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.|
References for anti-Tumor Necrosis Factor Antibody (ABIN1690740)Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
|Product cited in:||
Cesaro-Tadic, Dernick, Juncker et al.: "High-sensitivity miniaturized immunoassays for tumor necrosis factor alpha using microfluidic systems." in: Lab on a chip, Vol. 4, Issue 6, pp. 563-9, 2004 (PubMed).
Yan, Qing, Byers et al.: "Role of MyD88 in diminished tumor necrosis factor alpha production by newborn mononuclear cells in response to lipopolysaccharide." in: Infection and immunity, Vol. 72, Issue 3, pp. 1223-9, 2004 (PubMed).
Attarbaschi, Willheim, Ramharter et al.: "T cell cytokine profile during primary Epstein-Barr virus infection (infectious mononucleosis)." in: European cytokine network, Vol. 14, Issue 1, pp. 34-9, 2003 (PubMed).
Visser, Graffelman, Blauw et al.: "LPS-induced IL-10 production in whole blood cultures from chronic fatigue syndrome patients is increased but supersensitive to inhibition by dexamethasone." in: Journal of neuroimmunology, Vol. 119, Issue 2, pp. 343-9, 2001 (PubMed).
Wahlström, Katchar, Wigzell et al.: "Analysis of intracellular cytokines in CD4+ and CD8+ lung and blood T cells in sarcoidosis." in: American journal of respiratory and critical care medicine, Vol. 163, Issue 1, pp. 115-21, 2001 (PubMed).