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anti-Sheep (Ovine) Tumor Necrosis Factor antibody for Immunohistochemistry (Frozen Sections)

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Reactivity
Human, Mouse (Murine), Sheep (Ovine)
1214
441
336
139
132
118
107
55
55
31
25
18
13
8
8
7
7
4
4
4
3
2
2
1
1
1
Host
Mouse
918
487
201
44
13
9
7
6
3
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
168
95
69
49
32
30
27
26
25
25
25
25
25
25
24
24
24
18
13
9
4
4
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
891
806
593
306
254
237
206
183
130
128
101
63
27
25
22
22
18
14
8
8
6
6
5
5
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4360905
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3201318 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 78-234 Log in to see
1 ABIN1078625 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 78-234 Log in to see Polyclonal
1 ABIN4360908 FACS IHC IHC (fro) IHC (p) WB FITC Mouse IgG1 Log in to see 52B83
1 ABIN2476868 IHC (fro) WB Mouse IgG2a Log in to see VPM61 4

Top referenced anti-Tumor Necrosis Factor antibody for Immunohistochemistry (Frozen Sections)

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Sheep (Ovine) Mouse (Murine)
Cytometry by Time of Flight (CyTOF) 1 Antibodies 10 Antibodies
ELISA 12 Antibodies 208 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunohistochemistry (IHC) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies
Western Blotting (WB) 14 Antibodies
Biochemical Assay (BCA) 2 Antibodies
Blocking Antibody (Inhibition) 7 Antibodies
Blocking Peptide (BP) 3 Antibodies
Blocking Reagent (BR) 2 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA (Capture) 1 Antibodies
ELISpot 3 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human, Mouse (Murine), Sheep (Ovine)
(1214), (441), (336), (139), (132), (118), (107), (55), (55), (31), (25), (18), (13), (8), (8), (7), (7), (4), (4), (4), (3), (2), (2), (1), (1), (1)
Host Mouse
(918), (487), (201), (44), (13), (9), (7), (6), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(168), (95), (69), (49), (32), (30), (27), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (18), (13), (9), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(891), (806), (593), (306), (254), (237), (206), (183), (130), (128), (101), (63), (27), (25), (22), (22), (18), (14), (8), (8), (6), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN4360905) Images
Specificity This detects both natural and recombinant TNFa. It does not cross-react with TNF beta or lymphotoxin. Non-specific background staining is obesrved in connective tissues.
Purification Protein A or G purified
Immunogen The monoclonal antibody 52B83 reacts with tumor necrosis factor alpha (TNF-alpha).
Clone 52B83
Isotype IgG1

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN4360905) Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background Gene Symbol: TNF
Gene ID 7124
Research Area Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN4360905) Images back to top
Application Notes Western Blot 1:10-1:100, Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha (17.4 kDa) is not biologically active.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN4360905) Images back to top
Format Liquid
Concentration 0.1 mg/mL
Buffer PBS with 0.1 % BSA
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-Tumor Necrosis Factor Antibody (ABIN4360905)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Azeez, Olopade, Laperchia, Andrioli, Scambi, Onwuka, Bentivoglio, Olopade: "Regional Myelin and Axon Damage and Neuroinflammation in the Adult Mouse Brain After Long-Term Postnatal Vanadium Exposure." in: Journal of neuropathology and experimental neurology, Vol. 75, Issue 9, pp. 843-54, 2016 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Li, Das, Tang, Han, Jaiswal, Ray: "Transmembrane TNF-α Facilitates HIV-1 Infection of Podocytes Cultured from Children with HIV-Associated Nephropathy." in: Journal of the American Society of Nephrology : JASN, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Human).