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anti-Zebrafish Tumor Necrosis Factor antibody for Immunohistochemistry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Epitope
AA 115-130
37
30
27
24
23
23
17
17
15
12
10
10
10
7
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
1228
445
338
138
131
117
117
55
55
31
26
18
8
8
7
7
4
4
4
4
2
2
1
1
1
1
Host
Mouse
929
491
201
44
13
9
7
6
3
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
168
98
69
49
32
30
27
26
25
25
25
25
25
25
24
24
24
18
13
9
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
887
809
595
299
266
230
206
186
141
128
101
63
27
26
25
22
18
14
8
8
6
6
5
5
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN3023991
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3026343 FACS IF IHC Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8
1 ABIN3026344 FACS IF IHC Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8
1 ABIN1884226 IHC WB Rabbit Log in to see Polyclonal 1
1 ABIN3023961 IHC Mouse IgM AA 115-130, N-Term Log in to see TNF706-P-T2
1 ABIN3023992 FACS IF IHC Mouse IgG1, kappa AA 115-130 Log in to see SPM543

Top referenced anti-Tumor Necrosis Factor antibody for Immunohistochemistry

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Human Cat (Feline) Dog (Canine) Mouse (Murine) Rabbit Rat (Rattus) Zebrafish
Activity Inhibition (Act Inh) 2 Antibodies
Cytometry by Time of Flight (CyTOF) 27 Antibodies 10 Antibodies 10 Antibodies 10 Antibodies 10 Antibodies 18 Antibodies 10 Antibodies
Immunohistochemistry (Free Floating) (IHC (ff)) 2 Antibodies 2 Antibodies 2 Antibodies
Western Blotting (WB) 522 Antibodies 242 Antibodies 142 Antibodies 57 Antibodies 58 Antibodies 49 Antibodies 49 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Radioimmunoassay (RIA) 6 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 115-130
(37), (30), (27), (24), (23), (23), (17), (17), (15), (12), (10), (10), (10), (7), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
(1228), (445), (338), (138), (131), (117), (117), (55), (55), (31), (26), (18), (8), (8), (7), (7), (4), (4), (4), (4), (2), (2), (1), (1), (1), (1)
Host Mouse
(929), (491), (201), (44), (13), (9), (7), (6), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(168), (98), (69), (49), (32), (30), (27), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (18), (13), (9), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
(887), (809), (595), (299), (266), (230), (206), (186), (141), (128), (101), (63), (27), (26), (25), (22), (18), (14), (8), (8), (6), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Purification Protein G affinity chromatography
Immunogen Amino acids 115-130 of the human protein was used as the immunogen for this anti-TNF alpha antibody.
Clone SPM543
Isotype IgG1, kappa

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Research Area Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes The optimal dilution of the anti-TNF-alpha antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris Buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment Store the anti-TNF-alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor antibody (TNF) (AA 115-130) (ABIN3023991) Formalin-fixed, paraffin-embedded human polyostotic sclerosing histiocytosis (Erdheim...