anti-Horse (Equine) APAF1 antibody for Western Blotting

Recommended APAF1 Antibody (supplied by: Log in to see )

Antigen
Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies
  • APAF1
  • ARK
  • Apaf-1
  • Apaf1
  • CG6829
  • D-Apaf-1
  • DARK
  • Dapaf-1
  • Dapaf-1/HAC-1
  • Dark
  • Dark/Apaf-I
  • Dark/Dapaf-1/HAC1
  • Dark/Hac-1/dApaf-1
  • Dark/Hac-1/dApaf1
  • Dmel\\CG6829
  • Hac-1
  • Hac-1/Dark
  • Hac1
  • T1
  • anon-53Fa
  • apaf-1
  • apaf1
  • arc
  • ark
  • dAPAF-1
  • dApaf-1
  • dApaf-1/DARK/HAC-1
  • dApaf1
  • dArk
  • dapaf
  • dapaf-1
  • dapaf-1L
  • dapaf-1S
  • dark
  • dark/dapaf-1/hac-1
  • dark/hac-1/dapaf-1
  • hac-1
  • hac1
  • l(2)SH0173
  • APAF-1
  • CED4
  • 6230400I06Rik
  • Apaf1l
  • fog
  • mKIAA0413
  • zgc:136543
  • Apaf-1-related-killer
  • apoptotic peptidase activating factor 1
  • apoptotic protease activating factor 1
  • Ark
  • apaf1
  • APAF1
  • Apaf1
Reactivity
Cow (Bovine), Horse (Equine), Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus)
194
128
109
6
3
3
2
2
2
1
Host
Rabbit
165
24
13
3
Clonality
Polyclonal
Conjugate
This APAF1 antibody is un-conjugated
3
3
3
2
2
2
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
159
71
60
58
36
22
21
17
8
5
4
3
Options
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Catalog No. ABIN222876
$ 265.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN241600 WB Rabbit Log in to see Polyclonal
1 ABIN1001787 WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-APAF1 antibody for Western Blotting

Similar anti-APAF1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Horse (Equine) Monkey Rabbit
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 1 Antibodies
Western Blotting (WB) 149 Antibodies 91 Antibodies 72 Antibodies 3 Antibodies 3 Antibodies 7 Antibodies 4 Antibodies
Immunoprecipitation (IP) 30 Antibodies 20 Antibodies 14 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 58 Antibodies 36 Antibodies 33 Antibodies

General

Antigen Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies
Reactivity Cow (Bovine), Horse (Equine), Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus)
(194), (128), (109), (6), (3), (3), (2), (2), (2), (1)
Host Rabbit
(165), (24), (13), (3)
Clonality
Polyclonal
Conjugate This APAF1 antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Western Blotting (WB)
(159), (71), (60), (58), (36), (22), (21), (17), (8), (5), (4), (3)
Pubmed 4 references available
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Product Details anti-APAF1 Antibody

Target Details APAF1 Application Details Handling ProductDetails: References for anti-APAF1 antibody (ABIN222876) Images
Specificity The immunoaffinity purified antibody detects a 130 kDa protein, corresponding to the apparent molecular weight of Apaf-1 on SDS-PAGE immunoblots.
Purification Affinity purified
Immunogen Synthetic peptide surrounding amino acid 1161 of human Apaf-1
Isotype IgG

Target Details APAF1

Product Details anti-APAF1 Antibody Application Details Handling ProductDetails: References for anti-APAF1 antibody (ABIN222876) Images back to top
Antigen
Alternative Name Apaf-1 (APAF1 Antibody Abstract)
Background During apoptosis, Apaf-1 (apoptosis protease activating factor 1) binds to cytochrome c (Apaf-2), a reaction that requires dATP, and this leads to the formation of a large multimeric complex. The complex recruits pro-caspase-9, and activates caspase-9 (Apaf-3). Activated caspase-9 in turn cleaves and activates caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf-1 is ubiquitously expressed in human tissues.
Synonyms: APAF1, CED4, DKFZp781B1145, KIAA0413
Gene ID 20141188
UniProt O14727
Research Area Apoptosis/Necrosis
Pathways p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-APAF1 Antibody Target Details APAF1 Handling ProductDetails: References for anti-APAF1 antibody (ABIN222876) Images back to top
Application Notes Western blotting (0.5-4 μg/mL). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects a 130 kDa protein, corresponding to the apparent molecular weight of Apaf-1 on SDS-PAGE immunoblots.
Restrictions For Research Use only

Handling

Product Details anti-APAF1 Antibody Target Details APAF1 Application Details ProductDetails: References for anti-APAF1 antibody (ABIN222876) Images back to top
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) immunoaffinity purified, rabbit anti-Apaf-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.05 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

ProductDetails: References for anti-APAF1 antibody (ABIN222876)

Product Details anti-APAF1 Antibody Target Details APAF1 Application Details Handling Images back to top
Product cited in:

Morikawa, Nagahiro, Endo, Sato, Nakashima, Takatani, Mitsumori: "[Intralobar sequestration in elderly patient, report of a case]" in: Kyobu geka. The Japanese journal of thoracic surgery, Vol. 61, Issue 9, pp. 812-5, 2008

Siu, Alway: "Mitochondria-associated apoptotic signalling in denervated rat skeletal muscle." in: The Journal of physiology, Vol. 565, Issue Pt 1, pp. 309-23, 2005

Dirks, Leeuwenburgh: "Apoptosis in skeletal muscle with aging." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 282, Issue 2, pp. R519-27, 2002

Phaneuf, Leeuwenburgh: "Cytochrome c release from mitochondria in the aging heart: a possible mechanism for apoptosis with age." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 282, Issue 2, pp. R423-30, 2002

Images

Product Details anti-APAF1 Antibody Target Details APAF1 Application Details Handling ProductDetails: References for anti-APAF1 antibody (ABIN222876) back to top
Supplier Images
Western Blotting (WB) image for anti-APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (ABIN222876) Western blot analysis of Apaf1 expression in human heart tissue lysate.