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anti-Rat (Rattus) APAF1 antibody for Immunocytochemistry

Recommended APAF1 Antibody (supplied by: Log in to see )

Antigen
Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies
  • 6230400I06Rik
  • anon-53Fa
  • apaf-1
  • Apaf-1
  • APAF-1
  • Apaf1
  • apaf1
  • APAF1
  • Apaf1l
  • arc
  • ARK
  • ark
  • CED4
  • CG6829
  • D-Apaf-1
  • dapaf
  • Dapaf-1
  • dAPAF-1
  • dapaf-1
  • dApaf-1
  • dApaf-1/DARK/HAC-1
  • Dapaf-1/HAC-1
  • dapaf-1L
  • dapaf-1S
  • dApaf1
  • DARK
  • dark
  • dArk
  • Dark
  • Dark/Apaf-I
  • dark/dapaf-1/hac-1
  • Dark/Dapaf-1/HAC1
  • Dark/Hac-1/dApaf-1
  • dark/hac-1/dapaf-1
  • Dark/Hac-1/dApaf1
  • Dmel\\CG6829
  • fog
  • hac-1
  • Hac-1
  • Hac-1/Dark
  • hac1
  • Hac1
  • l(2)SH0173
  • mKIAA0413
  • T1
  • zgc:136543
Epitope
AA 1-97, N-Term
46
43
17
13
13
7
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus)
202
110
90
4
2
2
2
1
1
1
Host
Rat
173
25
12
4
Clonality
Monoclonal
Conjugate
This APAF1 antibody is un-conjugated
3
3
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
158
71
63
52
33
22
20
13
9
5
4
Supplier
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Catalog No. ABIN2745509
$ 351.75
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009962841 ABIN1731833 ICC IF IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0009962841 ABIN4280957 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
0.0009962841 ABIN4280959 ELISA ICC IHC Mouse Log in to see 5-00E11
0.0009962841 ABIN4280952 ICC IF IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
0.0009962841 ABIN4280958 ELISA ICC IHC WB Mouse Log in to see 2-00E10
0.0009962841 ABIN4280961 ELISA ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal

Similar anti-APAF1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 31 Antibodies 39 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 7 Antibodies 14 Antibodies
Immunofluorescence (IF) 5 Antibodies 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 32 Antibodies 33 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies 25 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Western Blotting (WB) 56 Antibodies
Immunochromatography (IC) 2 Antibodies

General

Antigen Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies
Epitope AA 1-97, N-Term
(46), (43), (17), (13), (13), (7), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine), Rat (Rattus)
(202), (110), (90), (4), (2), (2), (2), (1), (1), (1)
Host Rat
(173), (25), (12), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This APAF1 antibody is un-conjugated
(3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(158), (71), (63), (52), (33), (22), (20), (13), (9), (5), (4)
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Product Details anti-APAF1 Antibody

Target Details APAF1 Application Details Handling Images
Specificity Recognizes the CARD domain of mouse and rat Apaf-1.
Cross-Reactivity Mouse (Murine), Rat (Rattus)
No Cross-Reactivity Human
Cross-Reactivity (Details) Does not cross-react with human Apaf-1.
Purity >95 % (SDS-PAGE)
Endotoxin Level <0.1EU/μg purified protein (LAL test, Lonza).
Immunogen Recombinant mouse Apaf-1 (aa 1-97) containing the N-terminal CARD domain.
Clone 13F11
Isotype IgG2a

Target Details APAF1

Product Details anti-APAF1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Apaf-1 (APAF1 Antibody Abstract)
Background Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.
UniProt Q9EPV5
Research Area Apoptosis/Necrosis
Pathways p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-APAF1 Antibody Target Details APAF1 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-APAF1 Antibody Target Details APAF1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Liquid. In PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.
Expiry Date 12 months