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anti-Human ATM antibody for Immunohistochemistry

Recommended ATM Antibody (supplied by: Log in to see )

Antigen
Ataxia Telangiectasia Mutated (ATM) Antibodies
  • AI256621
  • at1
  • AT1
  • ATA
  • ATC
  • ATD
  • ATDC
  • atdc
  • ATE
  • Atm
  • ATM
  • atm
  • atm/tefu
  • C030026E19Rik
  • CG6535
  • dATM
  • Dmel\\CG6535
  • tef
  • Tefu
  • tel1
  • TEL1
  • TELO1
  • telo1
  • Xatm
Epitope
Ser1981
87
41
17
15
13
13
13
7
7
7
7
5
4
4
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
331
124
62
15
7
4
1
Host
Rabbit
218
87
17
8
3
Clonality
Monoclonal
Conjugate
This ATM antibody is un-conjugated
11
10
9
9
6
6
5
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
206
118
100
98
84
77
60
23
22
15
11
4
4
3
2
1
1
1
1
Supplier
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Available images

Catalog No. ABIN189039
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.22865479 ABIN1532809 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.22865479 ABIN1869998 IHC WB Rabbit IgG pSer1981 Log in to see Polyclonal
0.22865479 ABIN258963 ICC IF IHC (p) IP IHC WB Rabbit IgG Ser1981 Log in to see EP1890Y
0.22865479 ABIN2162810 IHC Rabbit IgG Ser1981 Log in to see Polyclonal
0.22865479 ABIN2469773 IP IHC WB Mouse IgG1 AA 980-1152 Log in to see 3-00E-008
0.22865479 ABIN2162811 IHC ELISA Rabbit IgG Ser1981 Log in to see Polyclonal
0.22865479 ABIN2156008 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.22865479 ABIN1030266 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.22865479 ABIN3020094 IHC WB Rabbit pSer1981 Log in to see Polyclonal
0.0011340706 ABIN251422 ICC IF IHC (p) IP IHC WB Mouse IgG1 kappa pSer1981 Log in to see 10H11-E12 14
0.0011340706 ABIN362100 IHC Rabbit IgG pSer1981 Log in to see Polyclonal 8
0.0011340706 ABIN151775 ELISA FACS ICC IF IHC IHC (p) IP WB Mouse IgG1 full length Log in to see 2C1 114
0.0011340706 ABIN1869997 IHC WB Rabbit IgG pSer1981 Log in to see Polyclonal
0.0011340706 ABIN151622 ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 5C2 24
0.0011340706 ABIN152102 ICC IF IHC (p) IP IHC PLA WB Rabbit AA 2550-2600 Log in to see Polyclonal
0.0011340706 ABIN965619 IHC Rabbit pSer1981 Log in to see 4
0.0011340706 ABIN1830256 IHC WB Sheep IgG AA 2138-2400 Log in to see Polyclonal
0.0011340706 ABIN1974379 IHC ELISA PE Rabbit IgG C-Term Log in to see Polyclonal
0.0011340706 ABIN1974380 IHC ELISA PE Rabbit IgG N-Term Log in to see Polyclonal
0.0011340706 ABIN221229 IF IP IHC WB Mouse IgG1 AA 2577-3056 Log in to see 2C1

Top referenced anti-ATM antibody for Immunohistochemistry

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Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
BioImaging (BI) 1 Antibodies
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
Dot Blot (DB) 22 Antibodies
ELISA 99 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 15 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 62 Antibodies
Immunofluorescence (IF) 87 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 121 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 79 Antibodies
Immunoprecipitation (IP) 102 Antibodies
Proximity Ligation Assay (PLA) 4 Antibodies
Western Blotting (WB) 209 Antibodies

General

Antigen Ataxia Telangiectasia Mutated (ATM) Antibodies
Epitope Ser1981
(87), (41), (17), (15), (13), (13), (13), (7), (7), (7), (7), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(331), (124), (62), (15), (7), (4), (1)
Host Rabbit
(218), (87), (17), (8), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ATM antibody is un-conjugated
(11), (10), (9), (9), (6), (6), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(206), (118), (100), (98), (84), (77), (60), (23), (22), (15), (11), (4), (4), (3), (2), (1), (1), (1), (1)
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Product Details anti-ATM Antibody

Target Details ATM Application Details Handling Images
Specificity Detects unphosphorylated ATM at Serine 1981.
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification IgG purified
Immunogen A synthetic peptide corresponding to residues surrounding Serine 1981 of human ATM was used as immunogen.
Clone Y170
Isotype IgG

Target Details ATM

Product Details anti-ATM Antibody Application Details Handling Images back to top
Antigen
Alternative Name ATM (ATM Antibody Abstract)
Background ATM is a nuclear phosphoprotein involved in Ataxia-telangiectasia (A-T) an autosomalrecessive disorder characterized by cerebellar ataxia, immune deficiencies, increasedcancer predisposition, chromosomal instability and radiation sensitivity (1-2). This largeprotein (370 kDa) is involved in genome stability, cellular responses to DNA damageand cell cycle control. More than 100 mutations have been identified so far and areexpected to inactivate the ATM protein by truncation or large deletions (3). Defects inATM contribute to various B cell and T cell Leukemia (4). Alternate Names: anti-AT complementation group A antibody, anti-AT complementation group C antibody,anti-AT complementation group D antibody, anti-AT complementation group E antibody,anti-AT mutated protein antibody, anti-AT1 antibody, anti-ATA antibody, anti-Ataxiatelangiectasia gene mutated antibody.
Gene Symbol: ATM
Gene ID 472, 11920, 300711
Research Area Cancer, DNA/RNA
Pathways p53 Signaling, Apoptosis, DNA Damage Repair

Application Details

Product Details anti-ATM Antibody Target Details ATM Handling Images back to top
Application Notes Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:100-250, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:100-250, Immunoprecipitation 1:50, Western Blot 1:1000-10000
Protocol Protocol specific for ATM Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-ATM Antibody Target Details ATM Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-ATM Antibody Target Details ATM Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-ATM antibody (Ataxia Telangiectasia Mutated) (Ser1981) (ABIN189039) Western blot analysis on 293 cell lysate using anti-ATM RabMAb (ABIN189039) dilution ...
Immunohistochemistry (IHC) image for anti-ATM antibody (Ataxia Telangiectasia Mutated) (Ser1981) (ABIN189039) Immunohistochemical analysis of paraffin-embedded human carcinoma using anti-ATM RabMAb
Immunofluorescence (IF) image for anti-ATM antibody (Ataxia Telangiectasia Mutated) (Ser1981) (ABIN189039) Immunofluorescent staining of 293 cells using anti-ATM RabMAb