anti-Human ATM antibody for Western Blotting

Recommended ATM Antibody (supplied by: Log in to see )

Antigen
Ataxia Telangiectasia Mutated (ATM) Antibodies
  • ATM
  • Atm
  • CG6535
  • Dmel\\CG6535
  • Tefu
  • atm
  • atm/tefu
  • dATM
  • tef
  • Xatm
  • at1
  • atdc
  • tel1
  • telo1
  • AT1
  • ATA
  • ATC
  • ATD
  • ATDC
  • ATE
  • TEL1
  • TELO1
  • AI256621
  • C030026E19Rik
  • telomere fusion
  • ataxia telangiectasia mutated
  • ataxia telangiectasia mutated (atm)
  • ataxia telangiectasia mutated homolog (human)
  • tefu
  • atm
  • ATM
  • EDI_100660
  • CpipJ_CPIJ001772
  • BDBG_08252
  • PAAG_02532
  • MCYG_05088
  • VDBG_06833
  • ACLA_015700
  • AaeL_AAEL014900
  • MGYG_07634
  • PGTG_14279
  • Atm
  • LOC100349299
Epitope
pSer1981
88
40
17
16
16
15
8
8
6
6
6
6
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
359
157
77
27
8
1
1
1
1
Host
Mouse
234
98
17
9
3
Clonality
Monoclonal
Conjugate
This ATM antibody is un-conjugated
12
11
10
9
6
6
5
5
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
219
125
110
87
85
71
58
45
27
21
13
6
4
4
2
2
2
1
1
Supplier
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Catalog No. ABIN151772
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.906104 ABIN151739 IP PLA WB Goat IgG AA 2550-2600 Log in to see Polyclonal 4
2.906104 ABIN151775 ChIP ELISA FACS ICC IF IHC IHC (p) IP WB Mouse IgG1 full length Log in to see 2C1 114
2.906104 ABIN151030 ICC FACS IF IHC (p) IP IHC ELISA WB Rabbit C-Term Log in to see Polyclonal 76
2.906104 ABIN3043719 WB Rabbit IgG AA 2870-3056 Log in to see Polyclonal
2.906104 ABIN151738 IP WB Goat IgG AA 1750-1800 Log in to see Polyclonal 2
2.906104 ABIN1449283 WB Mouse IgG1 Log in to see 4H1 3
2.906104 ABIN151622 ICC IF IHC IP WB Mouse IgG1 Log in to see 5C2 26
2.906104 ABIN152102 ICC IF IHC (p) IP IHC PLA WB Rabbit AA 2550-2600 Log in to see Polyclonal 2
2.906104 ABIN968969 ELISA WB Mouse IgG1 Log in to see 5C5 1
2.906104 ABIN3044170 WB Rabbit IgG AA 36-50, N-Term Log in to see Polyclonal
2.906104 ABIN1105463 EIA WB Mouse IgG1 Log in to see 5C5 1
2.906104 ABIN2705536 WB Rabbit pSer1981 Log in to see Polyclonal
2.906104 ABIN2705538 WB Rabbit Center Log in to see Polyclonal
2.906104 ABIN2705535 WB Rabbit pSer1981 Log in to see Polyclonal
2.906104 ABIN2705537 WB Rabbit pSer1987 Log in to see Polyclonal
2.906104 ABIN1869997 IF IHC WB Rabbit IgG pSer1981 Log in to see Polyclonal
2.906104 ABIN1869998 IHC WB Rabbit IgG pSer1981 Log in to see Polyclonal
2.906104 ABIN964571 ELISA WB Sheep IgG pSer1981 Log in to see Polyclonal
2.906104 ABIN964572 ELISA WB Sheep IgG pSer1981 Log in to see Polyclonal
2.906104 ABIN2957866 IHC WB Rabbit Log in to see Polyclonal

General

Antigen Ataxia Telangiectasia Mutated (ATM) Antibodies
Epitope pSer1981
(88), (40), (17), (16), (16), (15), (8), (8), (6), (6), (6), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(359), (157), (77), (27), (8), (1), (1), (1), (1)
Host Mouse
(234), (98), (17), (9), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ATM antibody is un-conjugated
(12), (11), (10), (9), (6), (6), (5), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(219), (125), (110), (87), (85), (71), (58), (45), (27), (21), (13), (6), (4), (4), (2), (2), (2), (1), (1)
Pubmed 19 references available
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Product Details anti-ATM Antibody

Target Details ATM Application Details Handling ProductDetails: References for anti-ATM antibody (ABIN151772) Images
No Cross-Reactivity Dog (Canine)
Purification Protein G purified
Immunogen Synthetic peptide made to a region surrounding the phosphorylated Serine 1981 of human ATM. [UniProt# Q13315]
Clone 10H11-E12
Isotype IgG1 kappa

Target Details ATM

Product Details anti-ATM Antibody Application Details Handling ProductDetails: References for anti-ATM antibody (ABIN151772) Images back to top
Antigen
Alternative Name ATM (ATM Antibody Abstract)
Background Gene Symbol: ATM
Molecular Weight Theoretical MW: 370 kDa
Gene ID 472
UniProt Q13315
Research Area DNA/RNA, Cancer
Pathways p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus

Application Details

Product Details anti-ATM Antibody Target Details ATM Handling ProductDetails: References for anti-ATM antibody (ABIN151772) Images back to top
Application Notes Western Blot 1:1000, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:1000, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:200This ATM [p Ser1981] (10H11.E12) antibody useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band at ~370 kDa can be seen. In IHC-P, staining was observed in the nucleus and cytoplasm of mouse spleen. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for ATM Kinase Antibody Western Blot Procedure1) Resolve protein samples on a 6 % SDS-PAGE gel at 185V for ~1.5 hours.
2) Transfer to PVDF membranes at 25V for ~1.5 hours.
) Block the membrane with TBST+BSA and goat serum for 1 hour at RT.
) Dilute primary antibody to 1:1,000 in blocking buffer.
) Incubate membrane overnight at 4 degrees Celcius in diluted anti-ATM-kinase.
) Wash 3 times ten minutes on a shaker.
) Incubate membranes with HRP conjugated anti-mouse IgG for 1 hour (RT), diluted in blocking buffer.
) Wash 3 times ten minutes on a shaker.
) Add ECL reagent, as per kit directions, and expose for 1-5 seconds.
Restrictions For Research Use only

Handling

Product Details anti-ATM Antibody Target Details ATM Application Details ProductDetails: References for anti-ATM antibody (ABIN151772) Images back to top
Format Liquid
Concentration 0.97 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

ProductDetails: References for anti-ATM antibody (ABIN151772)

Product Details anti-ATM Antibody Target Details ATM Application Details Handling Images back to top
Product cited in:

Liu, Liu, Gao, Reske, Li, Wu, Yang, Chen, Luo, Xiao: "A single non-synonymous NCOA5 variation in type 2 diabetic patients with hepatocellular carcinoma impairs the function of NCOA5 in cell cycle regulation." in: Cancer letters, Vol. 391, pp. 152-161, 2017 Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).

Wu, Wang, Chen, Chao, Chang, Chen, Hung: "Early Passage Mesenchymal Stem Cells Display Decreased Radiosensitivity and Increased DNA Repair Activity." in: Stem cells translational medicine, Vol. 6, Issue 6, pp. 1504-1514, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Takai, Jenkinson, Kabir, Babul-Hirji, Najm-Tehrani, Chitayat, Crow, de Lange: "A POT1 mutation implicates defective telomere end fill-in and telomere truncations in Coats plus." in: Genes & development, Vol. 30, Issue 7, pp. 812-26, 2016 (Sample species: Human).

Banerjee, Banerjee, Mandal: "Enhanced chemotherapeutic efficacy of apigenin liposomes in colorectal cancer based on flavone-membrane interactions." in: Journal of colloid and interface science, Vol. 491, pp. 98-110, 2016 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Yang, Shen, Oishi, Matteson, Tian, Goronzy, Weyand: "Restoring oxidant signaling suppresses proarthritogenic T cell effector functions in rheumatoid arthritis." in: Science translational medicine, Vol. 8, Issue 331, pp. 331ra38, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Liedtke, Biebernick, Radke, Stapelkamp, Coenen, Zaehres, Fritz, Kogler: "DNA damage response in neonatal and adult stromal cells compared with induced pluripotent stem cells." in: Stem cells translational medicine, Vol. 4, Issue 6, pp. 576-89, 2015 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Caserta, Egriboz, Wang, Martin, Koivisto, Pecót, Kladney, Shen, Shim, Pham, Karikomi, Mauntel, Majumder, Cuitino, Tang, Srivastava, Yu, Wallace, Mo, Park, Fernandez, Pilarski, La Perle, Rosol et al.: "Noncatalytic PTEN missense mutation predisposes to organ-selective cancer development in vivo. ..." in: Genes & development, Vol. 29, Issue 16, pp. 1707-20, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Sayin, Ibrahim, Larsson, Nilsson, Lindahl, Bergo: "Antioxidants accelerate lung cancer progression in mice." in: Science translational medicine, Vol. 6, Issue 221, pp. 221ra15, 2014 Further details: Western Blotting

Park, Jeon, Kim: "Selenoprotein W promotes cell cycle recovery from G2 arrest through the activation of CDC25B." in: Biochimica et biophysica acta, Vol. 1823, Issue 12, pp. 2217-26, 2012 (Sample species: Mouse (Murine)). Further details: Western Blotting

Someya, Sakata, Matsumoto, Tauchi, Kai, Hareyama, Fukushima: "Effects of depletion of dihydropyrimidine dehydrogenase on focus formation and RPA phosphorylation." in: Journal of radiation research, Vol. 53, Issue 2, pp. 250-6, 2012 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Guerra, Albihn, Tronnersjö, Yan, Guidi, Stenerlöw, Sterzenbach, Josenhans, Fox, Schauer, Thelestam, Larsson, Henriksson, Frisan: "Myc is required for activation of the ATM-dependent checkpoints in response to DNA damage." in: PLoS ONE, Vol. 5, Issue 1, pp. e8924, 2010 Further details: Western Blotting

Gastaldello, Hildebrand, Faridani, Callegari, Palmkvist, Di Guglielmo, Masucci: "A deneddylase encoded by Epstein-Barr virus promotes viral DNA replication by regulating the activity of cullin-RING ligases." in: Nature cell biology, Vol. 12, Issue 4, pp. 351-61, 2010

Caslini, Connelly, Serna, Broccoli, Hess: "MLL associates with telomeres and regulates telomeric repeat-containing RNA transcription." in: Molecular and cellular biology, Vol. 29, Issue 16, pp. 4519-26, 2009

Shao, Fujii, Colmegna, Oishi, Goronzy, Weyand: "Deficiency of the DNA repair enzyme ATM in rheumatoid arthritis." in: The Journal of experimental medicine, Vol. 206, Issue 6, pp. 1435-49, 2009

Yalcin, Zhang, Luciano, Mungamuri, Marinkovic, Vercherat, Sarkar, Grisotto, Taneja, Ghaffari: "Foxo3 is essential for the regulation of ataxia telangiectasia mutated and oxidative stress-mediated homeostasis of hematopoietic stem cells." in: The Journal of biological chemistry, Vol. 283, Issue 37, pp. 25692-705, 2008

Spardy, Duensing, Hoskins, Wells, Duensing: "HPV-16 E7 reveals a link between DNA replication stress, fanconi anemia D2 protein, and alternative lengthening of telomere-associated promyelocytic leukemia bodies." in: Cancer research, Vol. 68, Issue 23, pp. 9954-63, 2008

Luo, Rosenke, Czornak, Fortunato: "Human cytomegalovirus disrupts both ataxia telangiectasia mutated protein (ATM)- and ATM-Rad3-related kinase-mediated DNA damage responses during lytic infection." in: Journal of virology, Vol. 81, Issue 4, pp. 1934-50, 2007

Bakkenist, Drissi, Wu, Kastan, Dome: "Disappearance of the telomere dysfunction-induced stress response in fully senescent cells." in: Cancer research, Vol. 64, Issue 11, pp. 3748-52, 2004

Bakkenist, Kastan: "DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation." in: Nature, Vol. 421, Issue 6922, pp. 499-506, 2003

Images

Product Details anti-ATM Antibody Target Details ATM Application Details Handling ProductDetails: References for anti-ATM antibody (ABIN151772) back to top
Supplier Images
Western Blotting (WB) image for anti-Ataxia Telangiectasia Mutated (ATM) (pSer1981) antibody (ABIN151772) Western Blot analysis of ATM-kinase, using ABIN151772. Sample: Irradiated or peroxida...
Immunohistochemistry (IHC) image for anti-Ataxia Telangiectasia Mutated (ATM) (pSer1981) antibody (ABIN151772) Immunohistochemistry: ATM [p Ser1981] Antibody (10H11.E12) [ABIN151772] - ATM antibod...