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anti-Human ATM antibody for Western Blotting

Recommended ATM Antibody (supplied by: Log in to see )

Antigen
Ataxia Telangiectasia Mutated (ATM) Antibodies
  • AI256621
  • at1
  • AT1
  • ATA
  • ATC
  • ATD
  • ATDC
  • atdc
  • ATE
  • Atm
  • ATM
  • atm
  • atm/tefu
  • C030026E19Rik
  • CG6535
  • dATM
  • Dmel\\CG6535
  • tef
  • Tefu
  • tel1
  • TEL1
  • TELO1
  • telo1
  • Xatm
Epitope
pSer1981
86
41
17
15
13
13
13
7
7
7
7
5
4
4
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
330
123
61
15
7
4
1
Host
Mouse
218
86
17
8
3
Clonality
Monoclonal
Conjugate
This ATM antibody is un-conjugated
11
10
9
9
6
6
5
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
205
117
100
97
83
76
59
23
22
15
11
4
4
3
2
1
1
1
1
Supplier
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Catalog No. ABIN251422
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN1449283 WB Mouse IgG1 Log in to see 4H1 3
0.13341247 ABIN499359 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 1
0.13341247 ABIN968969 ELISA WB Mouse IgG1 Log in to see 5C5 1
0.13341247 ABIN151738 IP WB Goat IgG AA 1750-1800 Log in to see Polyclonal 2
0.13341247 ABIN189039 ICC IF IHC (p) IP IHC WB Rabbit IgG Ser1981 Log in to see Y170
0.13341247 ABIN1105463 EIA WB Mouse IgG1 Log in to see 5C5 1
0.13341247 ABIN3043719 WB Rabbit IgG AA 2870-3056 Log in to see Polyclonal
0.13341247 ABIN3044170 WB Rabbit IgG AA 36-50, N-Term Log in to see Polyclonal
0.13341247 ABIN151775 ELISA FACS ICC IF IHC IHC (p) IP WB Mouse IgG1 full length Log in to see 2C1 114
0.13341247 ABIN151739 IP PLA WB Goat IgG AA 2550-2600 Log in to see Polyclonal 4
0.13341247 ABIN964571 ELISA WB Sheep IgG pSer1981 Log in to see Polyclonal
0.13341247 ABIN964572 ELISA WB Sheep IgG pSer1981 Log in to see Polyclonal
0.13341247 ABIN1869998 IHC WB Rabbit IgG pSer1981 Log in to see Polyclonal
0.13341247 ABIN1869997 IHC WB Rabbit IgG pSer1981 Log in to see Polyclonal
0.13341247 ABIN2705535 WB Rabbit pSer1981 Log in to see Polyclonal
0.13341247 ABIN2705537 WB Rabbit pSer1987 Log in to see Polyclonal
0.13341247 ABIN2705538 WB Rabbit Center Log in to see Polyclonal
0.13341247 ABIN2705536 WB Rabbit pSer1981 Log in to see Polyclonal
0.13341247 ABIN258963 ICC IF IHC (p) IP IHC WB Rabbit IgG Ser1981 Log in to see EP1890Y
0.13341247 ABIN2162812 WB Rabbit IgG Ser1981 Log in to see Polyclonal

Top referenced anti-ATM antibody for Western Blotting

Similar anti-ATM Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Chromatin Immunoprecipitation (ChIP) 1 Antibodies 1 Antibodies
ELISA 15 Antibodies 29 Antibodies
Flow Cytometry (FACS) 2 Antibodies 13 Antibodies
Immunocytochemistry (ICC) 35 Antibodies 55 Antibodies
Immunofluorescence (IF) 35 Antibodies 62 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies 12 Antibodies
Immunohistochemistry (IHC) 35 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 39 Antibodies
Immunoprecipitation (IP) 35 Antibodies
Western Blotting (WB) 43 Antibodies
Biochemical Assay (BCA) 2 Antibodies
BioImaging (BI) 1 Antibodies

General

Antigen Ataxia Telangiectasia Mutated (ATM) Antibodies
Epitope pSer1981
(86), (41), (17), (15), (13), (13), (13), (7), (7), (7), (7), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(330), (123), (61), (15), (7), (4), (1)
Host Mouse
(218), (86), (17), (8), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ATM antibody is un-conjugated
(11), (10), (9), (9), (6), (6), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(205), (117), (100), (97), (83), (76), (59), (23), (22), (15), (11), (4), (4), (3), (2), (1), (1), (1), (1)
Pubmed 14 references available
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Product Details anti-ATM Antibody

Target Details ATM Application Details Handling References for anti-ATM Antibody (ABIN251422) Images
No Cross-Reactivity Dog (Canine)
Cross-Reactivity (Details) Does not work in canine per customer review
Purification Protein G purified
Immunogen Synthetic peptide made to a region surrounding the phosphorylated Serine 1981 of human ATM. [UniProt# Q13315]
Clone 10H11-E12
Isotype IgG1 kappa

Target Details ATM

Product Details anti-ATM Antibody Application Details Handling References for anti-ATM Antibody (ABIN251422) Images back to top
Antigen
Alternative Name ATM (ATM Antibody Abstract)
Background Gene Symbol: ATM
Molecular Weight Theoretical MW: 370 kDa
Gene ID 472, 300711
UniProt Q13315
Research Area DNA/RNA, Cancer
Pathways p53 Signaling, Apoptosis, DNA Damage Repair

Application Details

Product Details anti-ATM Antibody Target Details ATM Handling References for anti-ATM Antibody (ABIN251422) Images back to top
Application Notes Western Blot 1:1000, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:1000, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:200
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for ATM Kinase Antibody Western Blot Procedure1) Resolve protein samples on a 6 % SDS-PAGE gel at 185V for ~1.5 hours.
2) Transfer to PVDF membranes at 25V for ~1.5 hours.
) Block the membrane with TBST+BSA and goat serum for 1 hour at RT.
) Dilute primary antibody to 1:1,000 in blocking buffer.
) Incubate membrane overnight at 4 degrees Celcius in diluted anti-ATM-kinase.
) Wash 3 times ten minutes on a shaker.
) Incubate membranes with HRP conjugated anti-mouse IgG for 1 hour (RT), diluted in blocking buffer.
) Wash 3 times ten minutes on a shaker.
) Add ECL reagent, as per kit directions, and expose for 1-5 seconds.
Restrictions For Research Use only

Handling

Product Details anti-ATM Antibody Target Details ATM Application Details References for anti-ATM Antibody (ABIN251422) Images back to top
Format Liquid
Concentration 0.97 mg/mL
Buffer Tris-glycine, 150 mM NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-ATM Antibody (ABIN251422)

Product Details anti-ATM Antibody Target Details ATM Application Details Handling Images back to top
Product cited in:

Liedtke, Biebernick, Radke, Stapelkamp, Coenen, Zaehres, Fritz, Kogler: "DNA damage response in neonatal and adult stromal cells compared with induced pluripotent stem cells." in: Stem cells translational medicine, Vol. 4, Issue 6, pp. 576-89, 2015 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Caserta, Egriboz, Wang, Martin, Koivisto, Pecót, Kladney, Shen, Shim, Pham, Karikomi, Mauntel, Majumder, Cuitino, Tang, Srivastava, Yu, Wallace, Mo, Park, Fernandez, Pilarski, La Perle, Rosol et al.: "Noncatalytic PTEN missense mutation predisposes to organ-selective cancer development in vivo. ..." in: Genes & development, Vol. 29, Issue 16, pp. 1707-20, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Sayin, Ibrahim, Larsson, Nilsson, Lindahl, Bergo: "Antioxidants accelerate lung cancer progression in mice." in: Science translational medicine, Vol. 6, Issue 221, pp. 221ra15, 2014 Further details: Western Blotting

Park, Jeon, Kim: "Selenoprotein W promotes cell cycle recovery from G2 arrest through the activation of CDC25B." in: Biochimica et biophysica acta, Vol. 1823, Issue 12, pp. 2217-26, 2012 (Sample species: Mouse (Murine)). Further details: Western Blotting

Someya, Sakata, Matsumoto, Tauchi, Kai, Hareyama, Fukushima: "Effects of depletion of dihydropyrimidine dehydrogenase on focus formation and RPA phosphorylation." in: Journal of radiation research, Vol. 53, Issue 2, pp. 250-6, 2012 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Guerra, Albihn, Tronnersjö, Yan, Guidi, Stenerlöw, Sterzenbach, Josenhans, Fox, Schauer, Thelestam, Larsson, Henriksson, Frisan: "Myc is required for activation of the ATM-dependent checkpoints in response to DNA damage." in: PLoS ONE, Vol. 5, Issue 1, pp. e8924, 2010 Further details: Western Blotting

Gastaldello, Hildebrand, Faridani, Callegari, Palmkvist, Di Guglielmo, Masucci: "A deneddylase encoded by Epstein-Barr virus promotes viral DNA replication by regulating the activity of cullin-RING ligases." in: Nature cell biology, Vol. 12, Issue 4, pp. 351-61, 2010

Caslini, Connelly, Serna, Broccoli, Hess: "MLL associates with telomeres and regulates telomeric repeat-containing RNA transcription." in: Molecular and cellular biology, Vol. 29, Issue 16, pp. 4519-26, 2009

Shao, Fujii, Colmegna, Oishi, Goronzy, Weyand: "Deficiency of the DNA repair enzyme ATM in rheumatoid arthritis." in: The Journal of experimental medicine, Vol. 206, Issue 6, pp. 1435-49, 2009

Yalcin, Zhang, Luciano, Mungamuri, Marinkovic, Vercherat, Sarkar, Grisotto, Taneja, Ghaffari: "Foxo3 is essential for the regulation of ataxia telangiectasia mutated and oxidative stress-mediated homeostasis of hematopoietic stem cells." in: The Journal of biological chemistry, Vol. 283, Issue 37, pp. 25692-705, 2008

Spardy, Duensing, Hoskins, Wells, Duensing: "HPV-16 E7 reveals a link between DNA replication stress, fanconi anemia D2 protein, and alternative lengthening of telomere-associated promyelocytic leukemia bodies." in: Cancer research, Vol. 68, Issue 23, pp. 9954-63, 2008

Luo, Rosenke, Czornak, Fortunato: "Human cytomegalovirus disrupts both ataxia telangiectasia mutated protein (ATM)- and ATM-Rad3-related kinase-mediated DNA damage responses during lytic infection." in: Journal of virology, Vol. 81, Issue 4, pp. 1934-50, 2007

Bakkenist, Drissi, Wu, Kastan, Dome: "Disappearance of the telomere dysfunction-induced stress response in fully senescent cells." in: Cancer research, Vol. 64, Issue 11, pp. 3748-52, 2004

Bakkenist, Kastan: "DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation." in: Nature, Vol. 421, Issue 6922, pp. 499-506, 2003

Images

Product Details anti-ATM Antibody Target Details ATM Application Details Handling References for anti-ATM Antibody (ABIN251422) back to top
Supplier Images
Western Blotting (WB) image for anti-ATM antibody (Ataxia Telangiectasia Mutated) (pSer1981) (ABIN251422) Western Blot analysis of ATM-kinase, using ABIN151772. Sample: Irradiated or peroxida...