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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|Chromatin Immunoprecipitation (ChIP)||1 Antibodies||1 Antibodies|
|ELISA||2 Antibodies||9 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||4 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies||13 Antibodies|
|Immunofluorescence (IF)||4 Antibodies||20 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||11 Antibodies||11 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||12 Antibodies||12 Antibodies|
|Immunohistochemistry (IHC)||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies|
|Western Blotting (WB)||11 Antibodies|
|Biochemical Assay (BCA)||2 Antibodies|
|BioImaging (BI)||1 Antibodies|
|Antigen||Ataxia Telangiectasia Mutated (ATM) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This ATM antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-ATM AntibodyTarget Details ATM Application Details Handling References for anti-ATM Antibody (ABIN251422) Images
|No Cross-Reactivity||Dog (Canine)|
|Cross-Reactivity (Details)||Does not work in canine per customer review|
|Purification||Protein G purified|
|Immunogen||Synthetic peptide made to a region surrounding the phosphorylated Serine 1981 of human ATM.|
Target Details ATMProduct Details anti-ATM Antibody Application Details Handling References for anti-ATM Antibody (ABIN251422) Images back to top
|Alternative Name||ATM (ATM Antibody Abstract)|
ATM-kinase plays an important regulatory role. DNA damage caused by such things asionizing irradiation activates ATM-kinase, leading to a cascade of kinase reactions thatregulate cell cycle, apoptosis, and DNA damage repair. Alternate Names: anti-phosphorylated ATM antibody, anti-ATM S1981 antibody, anti-ATM phosphorylatedSer 1981 antibody, anti-ATM (phospho S1981) antibody.
Gene Symbol: ATM
|Gene ID||472, 300711|
|Pathways||p53 Signaling, Apoptosis, DNA Damage Repair|
Application DetailsProduct Details anti-ATM Antibody Target Details ATM Handling References for anti-ATM Antibody (ABIN251422) Images back to top
This ATM [p Ser1981] (10H11.E12) antibody useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band at ~370 kDa can be seen. In IHC-P, staining was observed in the nucleus and cytoplasm of mouse spleen.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:1000, Immunohistochemistry 1:200, Immunohistochemistry-Paraffin 1:200, Immunoprecipitation 1:10-1:500, Western Blot 1:1000
Protocol specific for ATM Kinase Antibody Western Blot Procedure1) Resolve protein samples on a 6 % SDS-PAGE gel at 185V for ~1.5 hours.
2) Transfer to PVDF membranes at 25V for ~1.5 hours.
) Block the membrane with TBST+BSA and goat serum for 1 hour at RT.
) Dilute primary antibody to 1:1,000 in blocking buffer.
) Incubate membrane overnight at 4 degrees Celcius in diluted anti-ATM-kinase.
) Wash 3 times ten minutes on a shaker.
) Incubate membranes with HRP conjugated anti-mouse IgG for 1 hour (RT), diluted in blocking buffer.
) Wash 3 times ten minutes on a shaker.
) Add ECL reagent, as per kit directions, and expose for 1-5 seconds.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ATM Antibody Target Details ATM Application Details References for anti-ATM Antibody (ABIN251422) Images back to top
|Buffer||Tris-glycine, 150 mM NaCl, Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||Aliquot and store at -20 °C or -80 °C.|
References for anti-ATM Antibody (ABIN251422)Product Details anti-ATM Antibody Target Details ATM Application Details Handling Images back to top
|Product cited in:||
Yalcin, Zhang, Luciano, Mungamuri, Marinkovic, Vercherat, Sarkar, Grisotto, Taneja, Ghaffari: "Foxo3 is essential for the regulation of ataxia telangiectasia mutated and oxidative stress-mediated homeostasis of hematopoietic stem cells." in: The Journal of biological chemistry, Vol. 283, Issue 37, pp. 25692-705, 2008
Spardy, Duensing, Hoskins, Wells, Duensing: "HPV-16 E7 reveals a link between DNA replication stress, fanconi anemia D2 protein, and alternative lengthening of telomere-associated promyelocytic leukemia bodies." in: Cancer research, Vol. 68, Issue 23, pp. 9954-63, 2008
Bakkenist, Kastan: "DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation." in: Nature, Vol. 421, Issue 6922, pp. 499-506, 2003
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