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anti-Mouse (Murine) ATM antibody for Western Blotting

Recommended ATM Antibody (supplied by: Log in to see )

Antigen
Ataxia Telangiectasia Mutated (ATM) Antibodies
  • AI256621
  • at1
  • AT1
  • ATA
  • ATC
  • ATD
  • ATDC
  • atdc
  • ATE
  • Atm
  • ATM
  • atm
  • atm/tefu
  • C030026E19Rik
  • CG6535
  • dATM
  • Dmel\\CG6535
  • tef
  • Tefu
  • tel1
  • TEL1
  • TELO1
  • telo1
  • Xatm
Epitope
pSer1981
86
37
17
14
13
13
13
7
7
7
5
5
4
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
321
114
59
14
6
4
1
Host
Mouse
214
84
14
8
3
Clonality
Monoclonal
Conjugate
This ATM antibody is un-conjugated
11
10
8
8
6
6
5
5
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
197
111
99
89
77
74
53
23
22
14
11
4
4
2
2
1
1
1
1
Supplier
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Catalog No. ABIN251422
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13398378 ABIN151738 IP WB Goat Log in to see Polyclonal 1
0.13398378 ABIN2705537 WB Rabbit pSer1987 Log in to see Polyclonal
0.13398378 ABIN2705538 WB Rabbit Center Log in to see Polyclonal
0.13398378 ABIN2705536 WB Rabbit pSer1981 Log in to see Polyclonal
0.13398378 ABIN2162812 WB Rabbit IgG Ser1981 Log in to see Polyclonal
0.13398378 ABIN2467775 WB Chicken AA 1248-1332 Log in to see Polyclonal
0.13398378 ABIN151739 PLA WB Goat Log in to see Polyclonal
0.13398378 ABIN151775 ICC IF IHC (p) IP IHC ELISA WB Mouse IgG1 AA 2577-3056 Log in to see 2C1
0.13398378 ABIN1866813 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
0.13398378 ABIN1882297 IF WB Rabbit Log in to see Polyclonal
0.13398378 ABIN349310 IF WB Rabbit IgG Log in to see Polyclonal
0.13398378 ABIN349309 IF WB Rabbit IgG Log in to see Polyclonal
0.0011731827 ABIN319313 IF WB Rabbit Ser1981 Log in to see Polyclonal 4
0.0011731827 ABIN362751 IF WB Rabbit IgG AA 1979-1983 Log in to see Polyclonal 5
0.0011731827 ABIN2688845 BI WB Mouse IgG2b, kappa pSer1981 Log in to see K88 2
0.0011731827 ABIN1583865 ELISA WB Rabbit Middle Region Log in to see Polyclonal
0.0011731827 ABIN223482 IP WB Rabbit IgG Log in to see Polyclonal
0.0011731827 ABIN363380 ICC FACS IF IP IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0011731827 ABIN121488 WB Rabbit AA 1-308, N-Term Log in to see Polyclonal
0.0011731827 ABIN121486 IHC (p) WB Rabbit AA 1980-2338 Log in to see Polyclonal

Top referenced anti-ATM antibody for Western Blotting

Similar anti-ATM Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Chromatin Immunoprecipitation (ChIP) 1 Antibodies 1 Antibodies
ELISA 15 Antibodies 29 Antibodies
Flow Cytometry (FACS) 1 Antibodies 12 Antibodies
Immunocytochemistry (ICC) 34 Antibodies 50 Antibodies
Immunofluorescence (IF) 34 Antibodies 57 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies 12 Antibodies
Immunohistochemistry (IHC) 34 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 38 Antibodies
Immunoprecipitation (IP) 35 Antibodies
Western Blotting (WB) 42 Antibodies
Biochemical Assay (BCA) 2 Antibodies
BioImaging (BI) 1 Antibodies

General

Antigen Ataxia Telangiectasia Mutated (ATM) Antibodies
Epitope pSer1981
(86), (37), (17), (14), (13), (13), (13), (7), (7), (7), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(321), (114), (59), (14), (6), (4), (1)
Host Mouse
(214), (84), (14), (8), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ATM antibody is un-conjugated
(11), (10), (8), (8), (6), (6), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(197), (111), (99), (89), (77), (74), (53), (23), (22), (14), (11), (4), (4), (2), (2), (1), (1), (1), (1)
Pubmed 14 references available
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Product Details anti-ATM Antibody

Target Details ATM Application Details Handling References for anti-ATM Antibody (ABIN251422) Images
No Cross-Reactivity Dog (Canine)
Cross-Reactivity (Details) Does not work in canine per customer review
Purification Protein G purified
Immunogen Synthetic peptide made to a region surrounding the phosphorylated Serine 1981 of human ATM.
Clone 10H11-E12
Isotype IgG1 kappa

Target Details ATM

Product Details anti-ATM Antibody Application Details Handling References for anti-ATM Antibody (ABIN251422) Images back to top
Antigen
Alternative Name ATM (ATM Antibody Abstract)
Background ATM-kinase plays an important regulatory role. DNA damage caused by such things asionizing irradiation activates ATM-kinase, leading to a cascade of kinase reactions thatregulate cell cycle, apoptosis, and DNA damage repair. Alternate Names: anti-phosphorylated ATM antibody, anti-ATM S1981 antibody, anti-ATM phosphorylatedSer 1981 antibody, anti-ATM (phospho S1981) antibody.
Gene Symbol: ATM
Gene ID 472, 300711
UniProt Q13315
Pathways p53 Signaling, Apoptosis, DNA Damage Repair

Application Details

Product Details anti-ATM Antibody Target Details ATM Handling References for anti-ATM Antibody (ABIN251422) Images back to top
Application Notes This ATM [p Ser1981] (10H11.E12) antibody useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band at ~370 kDa can be seen. In IHC-P, staining was observed in the nucleus and cytoplasm of mouse spleen.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:1000, Immunohistochemistry 1:200, Immunohistochemistry-Paraffin 1:200, Immunoprecipitation 1:10-1:500, Western Blot 1:1000
Protocol Protocol specific for ATM Kinase Antibody Western Blot Procedure1) Resolve protein samples on a 6 % SDS-PAGE gel at 185V for ~1.5 hours.
2) Transfer to PVDF membranes at 25V for ~1.5 hours.
) Block the membrane with TBST+BSA and goat serum for 1 hour at RT.
) Dilute primary antibody to 1:1,000 in blocking buffer.
) Incubate membrane overnight at 4 degrees Celcius in diluted anti-ATM-kinase.
) Wash 3 times ten minutes on a shaker.
) Incubate membranes with HRP conjugated anti-mouse IgG for 1 hour (RT), diluted in blocking buffer.
) Wash 3 times ten minutes on a shaker.
) Add ECL reagent, as per kit directions, and expose for 1-5 seconds.
Restrictions For Research Use only

Handling

Product Details anti-ATM Antibody Target Details ATM Application Details References for anti-ATM Antibody (ABIN251422) Images back to top
Format Liquid
Concentration 0.97 mg/mL
Buffer Tris-glycine, 150 mM NaCl, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.

References for anti-ATM Antibody (ABIN251422)

Product Details anti-ATM Antibody Target Details ATM Application Details Handling Images back to top
Product cited in:

Liedtke, Biebernick, Radke, Stapelkamp, Coenen, Zaehres, Fritz, Kogler: "DNA damage response in neonatal and adult stromal cells compared with induced pluripotent stem cells." in: Stem cells translational medicine, Vol. 4, Issue 6, pp. 576-89, 2015 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Caserta, Egriboz, Wang, Martin, Koivisto, Pecót, Kladney, Shen, Shim, Pham, Karikomi, Mauntel, Majumder, Cuitino, Tang, Srivastava, Yu, Wallace, Mo, Park, Fernandez, Pilarski, La Perle, Rosol et al.: "Noncatalytic PTEN missense mutation predisposes to organ-selective cancer development in vivo. ..." in: Genes & development, Vol. 29, Issue 16, pp. 1707-20, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Sayin, Ibrahim, Larsson, Nilsson, Lindahl, Bergo: "Antioxidants accelerate lung cancer progression in mice." in: Science translational medicine, Vol. 6, Issue 221, pp. 221ra15, 2014 Further details: Western Blotting

Park, Jeon, Kim: "Selenoprotein W promotes cell cycle recovery from G2 arrest through the activation of CDC25B." in: Biochimica et biophysica acta, Vol. 1823, Issue 12, pp. 2217-26, 2012 (Sample species: Mouse (Murine)). Further details: Western Blotting

Someya, Sakata, Matsumoto, Tauchi, Kai, Hareyama, Fukushima: "Effects of depletion of dihydropyrimidine dehydrogenase on focus formation and RPA phosphorylation." in: Journal of radiation research, Vol. 53, Issue 2, pp. 250-6, 2012 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Guerra, Albihn, Tronnersjö, Yan, Guidi, Stenerlöw, Sterzenbach, Josenhans, Fox, Schauer, Thelestam, Larsson, Henriksson, Frisan: "Myc is required for activation of the ATM-dependent checkpoints in response to DNA damage." in: PLoS ONE, Vol. 5, Issue 1, pp. e8924, 2010 Further details: Western Blotting

Gastaldello, Hildebrand, Faridani, Callegari, Palmkvist, Di Guglielmo, Masucci: "A deneddylase encoded by Epstein-Barr virus promotes viral DNA replication by regulating the activity of cullin-RING ligases." in: Nature cell biology, Vol. 12, Issue 4, pp. 351-61, 2010

Caslini, Connelly, Serna, Broccoli, Hess: "MLL associates with telomeres and regulates telomeric repeat-containing RNA transcription." in: Molecular and cellular biology, Vol. 29, Issue 16, pp. 4519-26, 2009

Shao, Fujii, Colmegna, Oishi, Goronzy, Weyand: "Deficiency of the DNA repair enzyme ATM in rheumatoid arthritis." in: The Journal of experimental medicine, Vol. 206, Issue 6, pp. 1435-49, 2009

Yalcin, Zhang, Luciano, Mungamuri, Marinkovic, Vercherat, Sarkar, Grisotto, Taneja, Ghaffari: "Foxo3 is essential for the regulation of ataxia telangiectasia mutated and oxidative stress-mediated homeostasis of hematopoietic stem cells." in: The Journal of biological chemistry, Vol. 283, Issue 37, pp. 25692-705, 2008

Spardy, Duensing, Hoskins, Wells, Duensing: "HPV-16 E7 reveals a link between DNA replication stress, fanconi anemia D2 protein, and alternative lengthening of telomere-associated promyelocytic leukemia bodies." in: Cancer research, Vol. 68, Issue 23, pp. 9954-63, 2008

Luo, Rosenke, Czornak, Fortunato: "Human cytomegalovirus disrupts both ataxia telangiectasia mutated protein (ATM)- and ATM-Rad3-related kinase-mediated DNA damage responses during lytic infection." in: Journal of virology, Vol. 81, Issue 4, pp. 1934-50, 2007

Bakkenist, Drissi, Wu, Kastan, Dome: "Disappearance of the telomere dysfunction-induced stress response in fully senescent cells." in: Cancer research, Vol. 64, Issue 11, pp. 3748-52, 2004

Bakkenist, Kastan: "DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation." in: Nature, Vol. 421, Issue 6922, pp. 499-506, 2003

Images

Product Details anti-ATM Antibody Target Details ATM Application Details Handling References for anti-ATM Antibody (ABIN251422) back to top
Supplier Images
Western Blotting (WB) image for anti-ATM antibody (Ataxia Telangiectasia Mutated) (pSer1981) (ABIN251422) Western Blot analysis of ATM-kinase, using ABIN151772. Sample: Irradiated or peroxida...