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anti-Human BAX antibody for Flow Cytometry

Recommended BAX Antibody (supplied by: Log in to see )

Antigen
BCL2-Associated X Protein (BAX) Antibodies
  • BAX
  • bax
  • bax-A
  • BAX-ALPHA
  • BCL2L4
  • fj16e01
  • wu:fc50b10
  • wu:fj16e01
  • xBax
  • xlbax
Epitope
Internal Region
114
63
30
26
18
15
15
13
13
12
12
11
9
9
8
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
490
167
155
65
23
22
13
9
3
2
2
2
1
1
Host
Rabbit
316
213
4
Clonality
Monoclonal
Conjugate
This BAX antibody is un-conjugated
19
14
13
10
8
7
7
6
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
388
202
153
143
138
129
98
66
56
34
6
6
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1687081
$ 594.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN2657050 FACS Alexa Fluor 488 Mouse IgG1 kappa Log in to see 2D2 7
0.30226976 ABIN2159471 FACS IHC WB Rabbit Ig Fraction Log in to see Polyclonal
0.0010482287 ABIN388094 FACS IHC (p) WB Rabbit Ig AA 41-76, BH3 Domain Log in to see Polyclonal 8
0.0010482287 ABIN371899 FACS IHC (p) Rabbit Internal Region Log in to see SP47
0.0010482287 ABIN2664009 FACS Rabbit IgG Log in to see Polyclonal 9
0.0010482287 ABIN2666258 FACS Mouse IgG1 kappa Log in to see 2D2 7
0.0010482287 ABIN2666260 FACS Mouse IgG1 kappa Log in to see 6A7 6
0.0010482287 ABIN2208719 FACS IF IP IHC ELISA WB Mouse IgG1 AA 3-16 Log in to see 2D2
0.0010482287 ABIN1976945 FACS IHC (p) Rabbit IgG Internal Region Log in to see SP47
0.0010482287 ABIN2657051 FACS Alexa Fluor 488 Mouse IgG1 kappa Log in to see 2D2 7
0.0010482287 ABIN451743 ELISA FACS ICC IF IHC IHC (fro) IP WB Mouse IgG1 kappa N-Term Log in to see 6A7 2
0.0010482287 ABIN2666259 FACS Mouse IgG1 kappa Log in to see 2D2 7
0.0010482287 ABIN2666261 FACS Mouse IgG1 kappa Log in to see 6A7 6
0.0010482287 ABIN2736349 FACS IF IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0010482287 ABIN1818409 ICC FACS IHC (p) IP WB Mouse IgG2b, kappa Log in to see 4F11
0.0010482287 ABIN4283104 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa AA 3-16 Log in to see 2D2
0.0010482287 ABIN4283105 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa AA 3-16 Log in to see 2D2
0.0010482287 ABIN4283106 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa AA 3-16 Log in to see 2D2
0.0010482287 ABIN4283107 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 AA 3-16 Log in to see SPM336
0.0010482287 ABIN4283108 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 AA 3-16 Log in to see SPM336

Top referenced anti-BAX antibody for Flow Cytometry

Similar anti-BAX Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 194 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 96 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 6 Antibodies
Immunocytochemistry (ICC) 64 Antibodies
Immunofluorescence (IF) 127 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 144 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 50 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 135 Antibodies
Immunoprecipitation (IP) 113 Antibodies

General

Antigen BCL2-Associated X Protein (BAX) Antibodies
Epitope Internal Region
(114), (63), (30), (26), (18), (15), (15), (13), (13), (12), (12), (11), (9), (9), (8), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(490), (167), (155), (65), (23), (22), (13), (9), (3), (2), (2), (2), (1), (1)
Host Rabbit
(316), (213), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BAX antibody is un-conjugated
(19), (14), (13), (10), (8), (7), (7), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(388), (202), (153), (143), (138), (129), (98), (66), (56), (34), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-BAX Antibody

Target Details BAX Application Details Handling Images
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus)
Characteristics Cellular Localization: Cytoplasm, Membrane
Purification Purified by Protein A/G
Immunogen Synthetic peptide corresponding to internal region of human Bax protein.
Clone SP47
Isotype IgG

Target Details BAX

Product Details anti-BAX Antibody Application Details Handling Images back to top
Antigen
Alternative Name BAX (BAX Antibody Abstract)
Background Bax is a member of the Bcl-2 family of proteins, which function as regulators of apoptosis. Overexpression of Bax functions to promote cell death. Bax can form homodimers and is also able to heterodimerize with other Bcl-2 related proteins.
Molecular Weight 21 kDa
Gene ID 581
Research Area Apoptosis/Necrosis
Pathways p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BAX Antibody Target Details BAX Handling Images back to top
Application Notes IHC Dilution: 1:50
Comment

Positive Control:
IHC (p): Normal breast
FC: HeLa Cell Line

Protocol IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilutions: If using the concentrate format of this product, dilute the antibody 1:50 prior to use. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at room temperature for 20 min. Primary Antibody: Incubate for 30 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
Restrictions For Research Use only

Handling

Product Details anti-BAX Antibody Target Details BAX Application Details Images back to top
Format Liquid
Buffer rabbit monoclonal antibody purified by protein A/G in PBS/1 % BSA buffer pH 7.6 with less than 0.1 sodium azide.
Preservative Sodium azide
Precaution of Use 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Storage 4 °C

Images

Product Details anti-BAX Antibody Target Details BAX Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-BAX antibody (BCL2-Associated X Protein) (Internal Region) (ABIN1687081) Flow cytometric analysis of rabbit anti-BAX (SP47) antibody in HeLa (green) compare t...
Immunohistochemistry (IHC) image for anti-BAX antibody (BCL2-Associated X Protein) (Internal Region) (ABIN1687081) anti-BCL2-Associated X Protein (BAX) (Internal Region) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-BAX antibody (BCL2-Associated X Protein) (Internal Region) (ABIN1687081) anti-BCL2-Associated X Protein (BAX) (Internal Region) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-BAX antibody (BCL2-Associated X Protein) (Internal Region) (ABIN1687081) anti-BCL2-Associated X Protein (BAX) (Internal Region) antibody (Image 4)
Immunohistochemistry (IHC) image for anti-BAX antibody (BCL2-Associated X Protein) (Internal Region) (ABIN1687081) anti-BCL2-Associated X Protein (BAX) (Internal Region) antibody (Image 5)
Immunohistochemistry (IHC) image for anti-BAX antibody (BCL2-Associated X Protein) (Internal Region) (ABIN1687081) anti-BCL2-Associated X Protein (BAX) (Internal Region) antibody (Image 6)
Immunohistochemistry (IHC) image for anti-BAX antibody (BCL2-Associated X Protein) (Internal Region) (ABIN1687081) anti-BCL2-Associated X Protein (BAX) (Internal Region) antibody (Image 7)
Immunohistochemistry (IHC) image for anti-BAX antibody (BCL2-Associated X Protein) (Internal Region) (ABIN1687081) anti-BCL2-Associated X Protein (BAX) (Internal Region) antibody (Image 8)
Immunohistochemistry (IHC) image for anti-BAX antibody (BCL2-Associated X Protein) (Internal Region) (ABIN1687081) anti-BCL2-Associated X Protein (BAX) (Internal Region) antibody (Image 9)
Immunohistochemistry (IHC) image for anti-BAX antibody (BCL2-Associated X Protein) (Internal Region) (ABIN1687081) anti-BCL2-Associated X Protein (BAX) (Internal Region) antibody (Image 10)
Immunohistochemistry (IHC) image for anti-BAX antibody (BCL2-Associated X Protein) (Internal Region) (ABIN1687081) anti-BCL2-Associated X Protein (BAX) (Internal Region) antibody (Image 11)