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|Application / Reactivity||Human|
|Cellular Assay (CA)||1 Antibodies|
|Dot Blot (DB)||1 Antibodies|
|Enzyme Immunoassay (EIA)||5 Antibodies|
|Flow Cytometry (FACS)||96 Antibodies|
|Fluorescence Microscopy (FM)||2 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Immunochromatography (IC)||6 Antibodies|
|Immunocytochemistry (ICC)||64 Antibodies|
|Immunofluorescence (IF)||127 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||34 Antibodies|
|Immunohistochemistry (IHC)||144 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||2 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||50 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||135 Antibodies|
|Immunoprecipitation (IP)||113 Antibodies|
|Antigen||BCL2-Associated X Protein (BAX) Antibodies|
|Epitope||Internal Region Alternatives|
|Conjugate||This BAX antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|Supplier||Log in to see|
Product Details anti-BAX AntibodyTarget Details BAX Application Details Handling Images
|Predicted Reactivity||Cow (Bovine),Mouse (Murine),Rat (Rattus)|
|Characteristics||Cellular Localization: Cytoplasm, Membrane|
|Purification||Purified by Protein A/G|
|Immunogen||Synthetic peptide corresponding to internal region of human Bax protein.|
Target Details BAXProduct Details anti-BAX Antibody Application Details Handling Images back to top
|Alternative Name||BAX (BAX Antibody Abstract)|
|Background||Bax is a member of the Bcl-2 family of proteins, which function as regulators of apoptosis. Overexpression of Bax functions to promote cell death. Bax can form homodimers and is also able to heterodimerize with other Bcl-2 related proteins.|
|Molecular Weight||21 kDa|
|Pathways||p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-BAX Antibody Target Details BAX Handling Images back to top
|Application Notes||IHC Dilution: 1:50|
IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilutions: If using the concentrate format of this product, dilute the antibody 1:50 prior to use. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at room temperature for 20 min. Primary Antibody: Incubate for 30 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
|Restrictions||For Research Use only|
HandlingProduct Details anti-BAX Antibody Target Details BAX Application Details Images back to top
|Buffer||rabbit monoclonal antibody purified by protein A/G in PBS/1 % BSA buffer pH 7.6 with less than 0.1 sodium azide.|
|Precaution of Use||
1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
|Handling Advice||Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.|
ImagesProduct Details anti-BAX Antibody Target Details BAX Application Details Handling back to top