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anti-Human GTPase NRas antibody for Western Blotting

Recommended GTPase NRas Antibody (supplied by: Log in to see )

Antigen
GTPase NRas (NRAS) Antibodies
  • ALPS4
  • AV095280
  • fj99a10
  • H-RAS
  • HRAS
  • N-RAS
  • N-ras
  • NRAS
  • NRAS1
  • NS6
  • wu:fj99a10
  • zgc:100761
  • zras-b1
Epitope
AA 72-101, N-Term
10
8
8
4
3
3
2
2
1
1
1
1
1
Reactivity
Human
70
19
14
4
1
Host
Rabbit
33
19
18
Clonality
Polyclonal
Conjugate
This GTPase NRas antibody is un-conjugated
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
64
40
20
19
9
5
5
2
1
1
1
Supplier
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Catalog No. ABIN392152
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN1882272 WB Mouse IgG1 AA 70-101, N-Term Log in to see 822CT17-5-1 1
0.12303223 ABIN781533 EIA WB Mouse IgG2a Log in to see AT2G9 3
0.12303223 ABIN1449328 IHC (p) WB Rabbit IgG Gln61Arg-Mutant Log in to see SP174
0.12303223 ABIN392153 FACS IHC (p) WB Rabbit Ig AA 147-179, C-Term Log in to see Polyclonal
0.12303223 ABIN189615 ICC IF IHC (p) IHC WB Rabbit IgG Log in to see Y131
0.12303223 ABIN2789436 WB Rabbit Middle Region Log in to see Polyclonal
0.12303223 ABIN2466026 IHC ELISA WB Goat Internal Region Log in to see Polyclonal
0.12303223 ABIN2449263 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2433487 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2447240 ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.12303223 ABIN2447241 IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.12303223 ABIN2444676 IHC WB Goat Log in to see Polyclonal
0.0011806369 ABIN375000 IHC (p) WB Goat Internal Region Log in to see Polyclonal 1
0.0011806369 ABIN360039 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 2
0.0011806369 ABIN302138 IHC ELISA WB Goat Internal Region Log in to see Polyclonal 1
0.0011806369 ABIN360038 EIA FACS IF WB Rabbit Ig N-Term Log in to see Polyclonal 2
0.0011806369 ABIN372821 EIA IHC (p) WB Goat IgG Internal Region Log in to see Polyclonal
0.0011806369 ABIN962114 IHC (p) ELISA WB Mouse IgG2a, kappa Log in to see AT2G9
0.0011806369 ABIN337227 IHC (p) ELISA WB Goat Internal Region Log in to see Polyclonal
0.0011806369 ABIN738336 IF (p) IHC (p) WB Rabbit IgG AA 166-186 Log in to see Polyclonal

Top referenced anti-GTPase NRas antibody for Western Blotting

Similar anti-GTPase NRas Antibodies

Application / Reactivity Human
ELISA 41 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 10 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 22 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 67 Antibodies

General

Antigen GTPase NRas (NRAS) Antibodies
Epitope AA 72-101, N-Term
(10), (8), (8), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(70), (19), (14), (4), (1)
Host Rabbit
(33), (19), (18)
Clonality (Clone)
Polyclonal   ( )
Conjugate This GTPase NRas antibody is un-conjugated
(1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
(64), (40), (20), (19), (9), (5), (5), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-GTPase NRas Antibody

Target Details GTPase NRas Application Details Handling References for anti-GTPase NRas Antibody (ABIN392152) Images
Specificity This NRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79~109 amino acids from the N-terminal region of human NRAS.
Predicted Reactivity Chicken,Mouse (Murine),Pig (Porcine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This NRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-101 AA from the N-terminal region of human NRAS.
Clone RB14459
Isotype Ig

Target Details GTPase NRas

Product Details anti-GTPase NRas Antibody Application Details Handling References for anti-GTPase NRas Antibody (ABIN392152) Images back to top
Antigen
Alternative Name NRAS (NRAS Antibody Abstract)
Background NRAS is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML).
Synonyms: GTPase NRas [Precursor], Transforming protein N-Ras, NRAS, HRAS1
Molecular Weight 21229 DA
Gene ID 4893
UniProt P01111
Pathways p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Handling References for anti-GTPase NRas Antibody (ABIN392152) Images back to top
Application Notes WB = 1:1000, FACS = 1:10-50, IF = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Application Details References for anti-GTPase NRas Antibody (ABIN392152) Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-GTPase NRas Antibody (ABIN392152)

Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Application Details Handling Images back to top
Background publications

Smalley, Contractor, Nguyen, Xiao, Edwards, Muthusamy, King, Flaherty, Bosenberg, Herlyn, Nathanson: "Identification of a novel subgroup of melanomas with KIT/cyclin-dependent kinase-4 overexpression." in: Cancer research, Vol. 68, Issue 14, pp. 5743-52, 2008

Images

Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Application Details Handling References for anti-GTPase NRas Antibody (ABIN392152) back to top
Supplier Images
Western Blotting (WB) image for anti-GTPase NRas antibody (NRAS) (AA 72-101) (ABIN392152) Western blot analysis of anti-NRAS Antibody (N-term) (ABIN392152) in CEM cell line ly...
Western Blotting (WB) image for anti-GTPase NRas antibody (NRAS) (AA 72-101) (ABIN392152) Western blot analysis of NRAS (arrow) using rabbit polyclonal NRAS Antibody (N-term) ...