You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human p300 antibody for Dot Blot

Recommended p300 Antibody (supplied by: Log in to see )

Antigen
E1A Binding Protein P300 (EP300) Antibodies
  • A430090G16
  • A730011L11
  • KAT3B
  • p300
  • p300 HAT
  • RSTS2
Epitope
pSer89
54
46
35
18
15
12
9
7
6
5
4
4
3
3
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
255
104
92
31
30
14
3
2
2
2
2
Host
Rabbit
158
103
Clonality
Polyclonal
Conjugate
This p300 antibody is un-conjugated
10
10
10
8
6
6
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Dot Blot (DB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
177
95
73
68
67
56
44
43
24
16
5
4
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN389618
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.637563 ABIN389698 DB IHC (p) Rabbit Ig pSer1834 Log in to see Polyclonal 1
0.637563 ABIN1881299 DB Rabbit pSer1834 Log in to see Polyclonal 5
0.637563 ABIN2160930 DB Rabbit Ig Fraction Log in to see Polyclonal
0.637563 ABIN2160931 DB IHC Rabbit Ig Fraction pSer89 Log in to see Polyclonal
0.00089372566 ABIN930935 DB ICC Mouse IgG1 Log in to see EP300G12
0.00089372566 ABIN1831757 DB ICC Mouse IgG1 Log in to see EP300G12
0.00089372566 ABIN1953028 DB IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Ser89, pSer89 Log in to see Polyclonal
0.00089372566 ABIN1952786 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG Ser1834, pSer1834 Log in to see Polyclonal
0.00089372566 ABIN1952787 DB IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Ser1834, pSer1834 Log in to see Polyclonal
0.00089372566 ABIN1953659 DB IHC ELISA WB APC Rabbit IgG Ser1834, pSer1834 Log in to see Polyclonal
0.00089372566 ABIN1953658 DB ELISA APC Rabbit IgG Ser1834, pSer1834 Log in to see Polyclonal
0.00089372566 ABIN1953900 DB IHC ELISA WB APC Rabbit IgG Ser89, pSer89 Log in to see Polyclonal
0.00089372566 ABIN1956104 DB ELISA FITC Rabbit IgG Ser1834, pSer1834 Log in to see Polyclonal
0.00089372566 ABIN1956105 DB IHC ELISA WB FITC Rabbit IgG Ser1834, pSer1834 Log in to see Polyclonal
0.00089372566 ABIN1956341 DB IHC ELISA WB FITC Rabbit IgG Ser89, pSer89 Log in to see Polyclonal
0.00089372566 ABIN1955166 DB IHC ELISA WB Biotin Rabbit IgG Ser89, pSer89 Log in to see Polyclonal
0.00089372566 ABIN1954956 DB IHC ELISA WB Biotin Rabbit IgG Ser1834, pSer1834 Log in to see Polyclonal
0.00089372566 ABIN1954955 DB ELISA Biotin Rabbit IgG Ser1834, pSer1834 Log in to see Polyclonal
0.00089372566 ABIN1958431 DB ELISA PE Rabbit IgG Ser1834, pSer1834 Log in to see Polyclonal
0.00089372566 ABIN1958432 DB IHC ELISA WB PE Rabbit IgG Ser1834, pSer1834 Log in to see Polyclonal

Top referenced anti-p300 antibody for Dot Blot

Similar anti-p300 Antibodies

Application / Reactivity Human
ChIP DNA-Sequencing (ChIPSeq) 2 Antibodies
Chromatin Immunoprecipitation (ChIP) 19 Antibodies
Dot Blot (DB) 57 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies
ELISA 92 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 52 Antibodies
Immunofluorescence (IF) 83 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 24 Antibodies
Immunohistochemistry (IHC) 68 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 43 Antibodies
Immunoprecipitation (IP) 79 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 186 Antibodies

General

Antigen E1A Binding Protein P300 (EP300) Antibodies
Epitope pSer89
(54), (46), (35), (18), (15), (12), (9), (7), (6), (5), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(255), (104), (92), (31), (30), (14), (3), (2), (2), (2), (2)
Host Rabbit
(158), (103)
Clonality (Clone)
Polyclonal   ( )
Conjugate This p300 antibody is un-conjugated
(10), (10), (10), (8), (6), (6), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Dot Blot (DB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(177), (95), (73), (68), (67), (56), (44), (43), (24), (16), (5), (4), (2), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-p300 Antibody

Target Details p300 Application Details Handling References for anti-p300 Antibody (ABIN389618) Images
Predicted Reactivity Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This EP300 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S89 of human EP300.
Clone RB7876
Isotype Ig

Target Details p300

Product Details anti-p300 Antibody Application Details Handling References for anti-p300 Antibody (ABIN389618) Images back to top
Antigen
Alternative Name EP300 (EP300 Antibody Abstract)
Background EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.

Synonyms: Histone acetyltransferase p300, E1A-associated protein p300, EP300, P300, KAT3B
Molecular Weight 264144 Da
Gene ID 2033
UniProt Q09472
Research Area Apoptosis/Necrosis, Signaling, Protein Modifications, Phospho-specific antibodies, Transcription Factors, Neurology
Pathways p53 Signaling, Notch Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-p300 Antibody Target Details p300 Handling References for anti-p300 Antibody (ABIN389618) Images back to top
Application Notes Suggested dilutions:
DB = 1:500, IHC (p) = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-p300 Antibody Target Details p300 Application Details References for anti-p300 Antibody (ABIN389618) Images back to top
Format Liquid
Concentration 0.45 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months

References for anti-p300 Antibody (ABIN389618)

Product Details anti-p300 Antibody Target Details p300 Application Details Handling Images back to top
Background publications

Finlan, Hupp: "The N-terminal interferon-binding domain (IBiD) homology domain of p300 binds to peptides with homology to the p53 transactivation domain." in: The Journal of biological chemistry, Vol. 279, Issue 47, pp. 49395-405, 2004

Kung, Zabludoff, France, Freedman, Tanner, Vieira, Cornell-Kennon, Lee, Wang, Wang, Memmert, Naegeli, Petersen, Eck, Bair, Wood, Livingston: "Small molecule blockade of transcriptional coactivation of the hypoxia-inducible factor pathway." in: Cancer cell, Vol. 6, Issue 1, pp. 33-43, 2004

Images

Product Details anti-p300 Antibody Target Details p300 Application Details Handling References for anti-p300 Antibody (ABIN389618) back to top
Supplier Images
Dot Blot (DB) image for anti-p300 antibody (E1A Binding Protein P300) (pSer89) (ABIN389618) Dot blot analysis of anti-Phospho-EP300-S89 Pab (ABIN389618) on nitrocellulose membra...
Immunohistochemistry (IHC) image for anti-p300 antibody (E1A Binding Protein P300) (pSer89) (ABIN389618) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...