anti-Human p300 antibody for Proximity Ligation Assay

Recommended p300 Antibody (supplied by: Log in to see )

Antigen
E1A Binding Protein P300 (EP300) Antibodies
  • KAT3B
  • RSTS2
  • p300
  • A430090G16
  • A730011L11
  • p300 HAT
  • E1A binding protein p300
  • E1A-associated protein p300
  • EP300
  • ep300
  • Ep300
Reactivity
Human
268
126
115
39
37
14
3
3
3
3
2
Host
Rabbit
164
111
Clonality
Polyclonal
Conjugate
This p300 antibody is un-conjugated
12
12
10
8
6
6
4
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Western Blotting (WB)
188
98
83
72
54
52
48
47
28
10
5
2
2
2
1
1
1
1
1
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Catalog No. ABIN152980
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1978817 ChIP IF IP IHC (p) PLA WB Rabbit IgG AA 950-1000 Log in to see Polyclonal

General

Antigen E1A Binding Protein P300 (EP300) Antibodies
Reactivity Human
(268), (126), (115), (39), (37), (14), (3), (3), (3), (3), (2)
Host Rabbit
(164), (111)
Clonality
Polyclonal
Conjugate This p300 antibody is un-conjugated
(12), (12), (10), (8), (6), (6), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (IHC), Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Western Blotting (WB)
(188), (98), (83), (72), (54), (52), (48), (47), (28), (10), (5), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-p300 Antibody

Target Details p300 Application Details Handling References for anti-p300 antibody (ABIN152980) Images
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to a portion of human E1A binding protein p300 encoded within exons 15 and 16 (LocusLink ID 2033).

Target Details p300

Product Details anti-p300 Antibody Application Details Handling References for anti-p300 antibody (ABIN152980) Images back to top
Antigen
Alternative Name KAT3B/p300 (EP300 Antibody Abstract)
Background Gene Symbol: EP300
Gene ID 2033
Pathways p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size

Application Details

Product Details anti-p300 Antibody Target Details p300 Handling References for anti-p300 antibody (ABIN152980) Images back to top
Application Notes Western Blot 1:5000-1:25000, Immunohistochemistry, Immunoprecipitation 1-4 μg/mg of lysate, Proximity Ligation Assay 1:1000-1:10000By Immunoprecipitation, this antibody recognizes a band at ~300 kDa, representing p300. Also useful for Western Blot. *The investigator should determine the optimal working dilution for a specific application.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-p300 Antibody Target Details p300 Application Details References for anti-p300 antibody (ABIN152980) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-p300 antibody (ABIN152980)

Product Details anti-p300 Antibody Target Details p300 Application Details Handling Images back to top
Product cited in:

Zhang, Vogel, Liu, Topark-Ngarm, Arbogast, Maier, Filtz, Leid: "Coordinated regulation of transcription factor Bcl11b activity in thymocytes by the mitogen-activated protein kinase (MAPK) pathways and protein sumoylation." in: The Journal of biological chemistry, Vol. 287, Issue 32, pp. 26971-88, 2012

Images

Product Details anti-p300 Antibody Target Details p300 Application Details Handling References for anti-p300 antibody (ABIN152980) back to top
Supplier Images
Western Blotting (WB) image for anti-E1A Binding Protein P300 (EP300) antibody (ABIN152980) anti-E1A Binding Protein P300 (EP300) antibody
Proximity Ligation Assay (PLA) image for anti-E1A Binding Protein P300 (EP300) antibody (ABIN152980) Proximity Ligation Assay: KAT3B/p300 Antibody [ABIN152980] - Secondary-conjugate Duol...
Proximity Ligation Assay (PLA) image for anti-E1A Binding Protein P300 (EP300) antibody (ABIN152980) Proximity Ligation Assay: KAT3B/p300 Antibody [ABIN152980] - Secondary-conjugate Duol...
Proximity Ligation Assay (PLA) image for anti-E1A Binding Protein P300 (EP300) antibody (ABIN152980) Proximity Ligation Assay: KAT3B/p300 Antibody [ABIN152980] - Secondary-conjugate Duol...
Proximity Ligation Assay (PLA) image for anti-E1A Binding Protein P300 (EP300) antibody (ABIN152980) Proximity Ligation Assay: KAT3B/p300 Antibody [ABIN152980] - Secondary-conjugate Duol...
Western Blotting (WB) image for anti-E1A Binding Protein P300 (EP300) antibody (ABIN152980) Western Blot: KAT3B/p300 Antibody - Detection of Human p300 by Western Blot. Samples...
Immunocytochemistry (ICC) image for anti-E1A Binding Protein P300 (EP300) antibody (ABIN152980) Immunohistochemistry: KAT3B/p300 Antibody - Sample: FFPE section of human lung carci...