You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Mink p300 antibody for Immunofluorescence

Recommended p300 Antibody (supplied by: Log in to see )

Antigen
E1A Binding Protein P300 (EP300) Antibodies
  • A430090G16
  • A730011L11
  • KAT3B
  • p300
  • p300 HAT
  • RSTS2
Epitope
AA 2071-2091
54
36
18
16
12
9
7
6
5
4
4
3
3
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mink, Monkey, Mouse (Murine), Rat (Rattus)
221
71
59
13
2
2
2
2
2
Host
Mouse
156
70
Clonality
Monoclonal
Conjugate
This p300 antibody is un-conjugated
10
10
8
6
6
6
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
145
94
64
57
42
40
37
24
13
5
3
3
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN180371
$ 589.05
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010850558 ABIN1722721 ICC IF IP WB Mouse IgG1 kappa AA 1572-2371 Log in to see RW128
0.0010850558 ABIN1722719 IF IP WB Mouse IgG1 kappa AA 1572-2371 Log in to see RW105

Top referenced anti-p300 antibody for Immunofluorescence

Similar anti-p300 Antibodies

Application / Reactivity Human Monkey Mouse (Murine) Rat (Rattus) Mink
Western Blotting (WB) 154 Antibodies 16 Antibodies 35 Antibodies 27 Antibodies 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 49 Antibodies 17 Antibodies 18 Antibodies 18 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 40 Antibodies 1 Antibodies 1 Antibodies 14 Antibodies 14 Antibodies
Immunohistochemistry (IHC) 65 Antibodies 8 Antibodies 7 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies

General

Antigen E1A Binding Protein P300 (EP300) Antibodies
Epitope AA 2071-2091
(54), (36), (18), (16), (12), (9), (7), (6), (5), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mink, Monkey, Mouse (Murine), Rat (Rattus)
(221), (71), (59), (13), (2), (2), (2), (2), (2)
Host Mouse
(156), (70)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p300 antibody is un-conjugated
(10), (10), (8), (6), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(145), (94), (64), (57), (42), (40), (37), (24), (13), (5), (3), (3), (2), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-p300 Antibody

Target Details p300 Application Details Handling References for anti-p300 Antibody (ABIN180371) Images
Characteristics Synonyms: CREBBP, P300, CREB-binding protein, EP300, P300 HAT, E1A binding protein p300, Histoneacetyltransferase p300, E1A-associated protein p300
Purification Affinity Chromatography on Protein A
Immunogen E1A affinity purified p300 protein. Remarks: Epitope is between aa 2071-2091.
Clone NM11
Isotype IgG2b

Target Details p300

Product Details anti-p300 Antibody Application Details Handling References for anti-p300 Antibody (ABIN180371) Images back to top
Antigen
Alternative Name EP300 / P300 (EP300 Antibody Abstract)
Background P300 is a transcriptional adapter protein which is characterized by three cysteine- and histidine-rich regions and its C-terminus specifically binds the adenovirus E1A protein. p300, and p300-associated proteins, are components of TATA-binding protein (TBP) complexes. Protein kinase A mediated CREB phosphorylation results in the binding of CREB to a 265 kDa nuclear protein designated CBP (for CREB-binding protein). CBP and p300 are homologous to each other.Synonyms: CREB-binding protein, CREBBP, E1A binding protein p300, E1A-associated protein p300, EP300, Histone acetyltransferase p300, P300, P300 HAT
Research Area Transcription Factors
Pathways p53 Signaling, Notch Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-p300 Antibody Target Details p300 Handling References for anti-p300 Antibody (ABIN180371) Images back to top
Application Notes Immunofluorescence. Immunoprecipitation (Native and denatured): Use Protein A and Ab at 2 μg/mg proteinlysate. Immunohistology (Formalin/Paraffin): Use Ab at 1/50-1/100 for 20 Minutes at RT. Stainingof formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for10-20 min followed by cooling at RT for 20 min. Recommended Positive Control: Ls174T Cells, Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-p300 Antibody Target Details p300 Application Details References for anti-p300 Antibody (ABIN180371) Images back to top
Concentration 0.2 mg/mL
Buffer 10 mM PBS pH 7.4 with 0.09 % Sodium Azide as preservative and 0.2 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freeze thaw cycles!
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-p300 Antibody (ABIN180371)

Product Details anti-p300 Antibody Target Details p300 Application Details Handling Images back to top
Background publications

Dallas, Yaciuk, Moran: "Characterization of monoclonal antibodies raised against p300: both p300 and CBP are present in intracellular TBP complexes." in: Journal of virology, Vol. 71, Issue 2, pp. 1726-31, 1997

Dallas, Yaciuk, Moran: "Monoclonal antibody NM11 recognizes a C-terminal epitope shared by p300 and CBP." in: Hybridoma, Vol. 16, Issue 3, pp. 273-5, 1997

Images

Product Details anti-p300 Antibody Target Details p300 Application Details Handling References for anti-p300 Antibody (ABIN180371) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-p300 antibody (E1A Binding Protein P300) (AA 2071-2091) (ABIN180371) anti-E1A Binding Protein P300 (EP300) (AA 2071-2091) antibody
Immunohistochemistry (IHC) image for anti-p300 antibody (E1A Binding Protein P300) (AA 2071-2091) (ABIN180371) anti-E1A Binding Protein P300 (EP300) (AA 2071-2091) antibody (Image 2)