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anti-Rat (Rattus) p300 antibody for Chromatin Immunoprecipitation

Recommended p300 Antibody (supplied by: Log in to see )

Antigen
E1A Binding Protein P300 (EP300) Antibodies
  • KAT3B
  • RSTS2
  • p300
  • A430090G16
  • A730011L11
  • p300 HAT
  • E1A binding protein p300
  • E1A-associated protein p300
  • EP300
  • ep300
  • Ep300
Epitope
AA 1572-2371, AA 2107-2283
56
52
37
18
15
11
6
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Mustelid, Primate, Rat (Rattus)
264
126
115
38
36
15
3
3
3
3
3
Host
Mouse
160
111
Clonality
Monoclonal
Conjugate
This p300 antibody is un-conjugated
12
12
10
8
6
6
4
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
186
96
81
70
54
51
51
46
28
9
5
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN152048
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN152047 ChIP EM FACS ICC IF IP in vitro WB Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105 8
1 ABIN4328401 ChIP ICC IF IP WB PE Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
1 ABIN4328403 ChIP ICC IF IP WB PerCP Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
1 ABIN4328405 ChIP ICC IF IP WB Allophycocyanin Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105

Top referenced anti-p300 antibody for Chromatin Immunoprecipitation

Similar anti-p300 Antibodies

Application / Reactivity Rat (Rattus) Primate
Chromatin Immunoprecipitation (ChIP) 5 Antibodies 5 Antibodies
Electron Microscopy (EM) 1 Antibodies 1 Antibodies
ELISA 11 Antibodies
Flow Cytometry (FACS) 2 Antibodies 2 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 42 Antibodies 36 Antibodies
Immunofluorescence (IF) 51 Antibodies 36 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 28 Antibodies
Immunohistochemistry (IHC) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 19 Antibodies 1 Antibodies
Immunoprecipitation (IP) 27 Antibodies
In vitro Assay (in vitro) 1 Antibodies
Western Blotting (WB) 70 Antibodies

General

Antigen E1A Binding Protein P300 (EP300) Antibodies
Epitope AA 1572-2371, AA 2107-2283
(56), (52), (37), (18), (15), (11), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Mustelid, Primate, Rat (Rattus)
(264), (126), (115), (38), (36), (15), (3), (3), (3), (3), (3)
Host Mouse
(160), (111)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p300 antibody is un-conjugated
(12), (12), (10), (8), (6), (6), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(186), (96), (81), (70), (54), (51), (51), (46), (28), (9), (5), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-p300 Antibody

Target Details p300 Application Details Handling References for anti-p300 Antibody (ABIN152048) Images
Specificity This is specific for p300 protein. This recognizes residues 2107-2283.
Purification Unpurified
Immunogen Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947]
Clone RW109
Isotype IgG1 kappa

Target Details p300

Product Details anti-p300 Antibody Application Details Handling References for anti-p300 Antibody (ABIN152048) Images back to top
Antigen
Alternative Name KAT3B/p300 (EP300 Antibody Abstract)
Background Gene Symbol: EP300
Molecular Weight Theoretical MW: 300 kDa
Gene ID 2033
Pathways p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size

Application Details

Product Details anti-p300 Antibody Target Details p300 Handling References for anti-p300 Antibody (ABIN152048) Images back to top
Application Notes Western Blot 1:1000, Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence 1:100-1:200, Immunoprecipitation 1:10-1:500This KAT3B/p300 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot where a band is seen at ~300 kDa. A lower non-specific MW bands (~50 kDa) may be seen with longer exposure times. Chromatin Immunoprecipitation was reported in scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for KAT3B / p300 Antibody Western Blot Protocol
1. Perform SDS-PAGE (3-8 % Tris-acetate) on samples to be analyzed, loading 50ug of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk in TBS for 1.5 hours.
. Dilute the mouse anti-p300 primary antibody in blocking buffer and incubate overnight at 4 °C.
. Wash the membrane in water for 5 minutes and apply the diluted mouse-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-p300 Antibody Target Details p300 Application Details References for anti-p300 Antibody (ABIN152048) Images back to top
Format Liquid
Buffer Ascites
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-p300 Antibody (ABIN152048)

Product Details anti-p300 Antibody Target Details p300 Application Details Handling Images back to top
Product cited in:

Romano, Staibano, Greco, Brunetti, Nappo, Ilardi, Martinelli, Sorrentino, Di Pace, Mascolo, Bisogni, Scalvenzi, Alfano, Romano: "FK506 binding protein 51 positively regulates melanoma stemness and metastatic potential." in: Cell death & disease, Vol. 4, pp. e578, 2013 (Sample species: Human). Further details: Western Blotting,Chromatin Immunoprecipitation,Immunoprecipitation

Eckner, Ludlow, Lill, Oldread, Arany, Modjtahedi, DeCaprio, Livingston, Morgan: "Association of p300 and CBP with simian virus 40 large T antigen." in: Molecular and cellular biology, Vol. 16, Issue 7, pp. 3454-64, 1996 (Sample species: Mouse (Murine)). Further details: Immunoprecipitation

Eckner, Yao, Oldread, Livingston: "Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation." in: Genes & development, Vol. 10, Issue 19, pp. 2478-90, 1996

Eckner, Ewen, Newsome, Gerdes, DeCaprio, Lawrence, Livingston: "Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor." in: Genes & development, Vol. 8, Issue 8, pp. 869-84, 1994 (Sample species: Human). Further details: Western Blotting

Images

Product Details anti-p300 Antibody Target Details p300 Application Details Handling References for anti-p300 Antibody (ABIN152048) back to top
Supplier Images
Western Blotting (WB) image for anti-p300 antibody (E1A Binding Protein P300) (AA 1572-2371) (ABIN152048) p300 detected in a HeLa nuclear extract using ABIN152048 (1:1,000). ECL: 20 minute e...