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|Application / Reactivity||Human||Rat (Rattus)||Monkey||Chicken||Dog (Canine)||Mouse (Murine)||Cow (Bovine)||Guinea Pig||Hamster||Pig (Porcine)||Rabbit||Sheep (Ovine)||Xenopus laevis|
|Immunofluorescence (fixed cells) (IF/ICC)||7 Antibodies||2 Antibodies||1 Antibodies||1 Antibodies||1 Antibodies||3 Antibodies|
|Western Blotting (WB)||347 Antibodies||144 Antibodies||111 Antibodies||27 Antibodies||33 Antibodies||28 Antibodies||23 Antibodies||22 Antibodies||37 Antibodies||25 Antibodies||22 Antibodies||22 Antibodies||24 Antibodies|
|Antigen||V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies|
|Reactivity||Cow (Bovine), Dog (Canine), Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis Alternatives|
|Conjugate||This AKT2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-AKT2 AntibodyTarget Details AKT2 Application Details Handling References for anti-AKT2 Antibody (ABIN264873) Images
|Specificity||This antibody detects a ~65 kDa protein, corresponding to the Molecular Mass of Akt2 PKB beta on SDS-PAGE immunoblots. Does not react with PKB-alpha (Akt1) or PKB-gamma (Akt3).|
|Immunogen||A five residue synthetic peptide based on the Human Akt2, coupled to KLH|
Target Details AKT2Product Details anti-AKT2 Antibody Application Details Handling References for anti-AKT2 Antibody (ABIN264873) Images back to top
|Alternative Name||AKT2 / PKB beta (AKT2 Antibody Abstract)|
|Background||Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).Synonyms: Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase|
|Pathways||PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C|
Application DetailsProduct Details anti-AKT2 Antibody Target Details AKT2 Handling References for anti-AKT2 Antibody (ABIN264873) Images back to top
Western blot (3): 1: 13000 dilution of SPC-148 was ufficient for detection of PKBβ in 20 μg ofHeLa cell lysate by ECL immunoblot analysis. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-AKT2 Antibody Target Details AKT2 Application Details References for anti-AKT2 Antibody (ABIN264873) Images back to top
|Buffer||TBS, 0.09 % Sodium Azide, 50 % Glycerol|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-AKT2 Antibody (ABIN264873)Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling Images back to top
Nicholson, Anderson: "The protein kinase B/Akt signalling pathway in human malignancy." in: Cellular signalling, Vol. 14, Issue 5, pp. 381-95, 2002
Brazil, Hemmings: "Ten years of protein kinase B signalling: a hard Akt to follow." in: Trends in biochemical sciences, Vol. 26, Issue 11, pp. 657-64, 2001
Downward: "Mechanisms and consequences of activation of protein kinase B/Akt." in: Current opinion in cell biology, Vol. 10, Issue 2, pp. 262-7, 1998
ImagesProduct Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling References for anti-AKT2 Antibody (ABIN264873) back to top