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anti-Dog (Canine) CDK2 antibody for Immunoprecipitation

Recommended CDK2 Antibody (supplied by: Log in to see )

Antigen
Cyclin-Dependent Kinase 2 (CDK2) Antibodies
  • A630093N05Rik
  • cdk2L
  • CDKN2
  • CINP
  • cinp
  • eg1
  • MGC84068
  • p33(CDK2)
  • wu:fa10c02
  • zgc:56598
  • zgc:77684
Epitope
AA 109-298
51
46
25
13
9
7
7
7
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
246
141
116
9
9
7
7
7
6
5
4
4
3
Host
Mouse
232
41
1
Clonality
Monoclonal
Conjugate
This CDK2 antibody is un-conjugated
11
8
7
4
4
4
3
3
3
3
3
3
3
3
3
Application
BioImaging (BI), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
211
102
66
44
40
33
33
30
12
11
9
8
3
1
1
1
1
1
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Catalog No. ABIN967777
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089235755 ABIN967776 BI IP IHC WB Mouse IgG2a AA 109-298 Log in to see 55-Cdk2 5
0.00089235755 ABIN2704626 IC IF IP IHC WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-CDK2 antibody for Immunoprecipitation

Similar anti-CDK2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
BioImaging (BI) 2 Antibodies 2 Antibodies
ELISA 30 Antibodies 43 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 4 Antibodies
Flow Cytometry (FACS) 1 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 7 Antibodies
Immunofluorescence (IF) 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 33 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies
Immunoprecipitation (IP) 11 Antibodies
Western Blotting (WB) 80 Antibodies
Biochemical Assay (BCA) 1 Antibodies

General

Antigen Cyclin-Dependent Kinase 2 (CDK2) Antibodies
Epitope AA 109-298
(51), (46), (25), (13), (9), (7), (7), (7), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(246), (141), (116), (9), (9), (7), (7), (7), (6), (5), (4), (4), (3)
Host Mouse
(232), (41), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CDK2 antibody is un-conjugated
(11), (8), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application BioImaging (BI), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(211), (102), (66), (44), (40), (33), (33), (30), (12), (11), (9), (8), (3), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CDK2 Antibody

Target Details CDK2 Application Details Handling References for anti-CDK2 Antibody (ABIN967777) Images
Cross-Reactivity Dog (Canine), Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
3. Triton is a trademark of the Dow Chemical Company.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Cdk2 aa. 109-298
Clone 55-Cdk2
Isotype IgG2a

Target Details CDK2

Product Details anti-CDK2 Antibody Application Details Handling References for anti-CDK2 Antibody (ABIN967777) Images back to top
Antigen
Alternative Name Cdk2 (CDK2 Antibody Abstract)
Background Cyclin-dependent kinase 2 (Cdk2) is a member of a family of cdc2-related cell cycle protein kinases. Cdk2 shares 60% identity with cdc2 and its activity is regulated by phosphorylation in a similar fashion. Cdk2 is expressed earlier in the cell cycle than is cdc2. Like p34 [cdc2], p33 [cdk2] associates with Cyclin A in human cells. However, kinase activity associated with Cyclin A-Cdk2 is present in S phase, whereas, the kinase activity associated with Cyclin A-cdc2 is found only in G2. Cdk2 can also complex with cyclins E, D1, and D3. It is not known if the D cyclins can form active complexes with Cdk2. Cyclin E-Cdk2 kinase is active in the G1 and S phases of the cell cycle and is important (as is Cyclin A-Cdk2) for the progression from G1 to S phase. The levels of Cyclin A-Cdk2 are maximal at the G1/S transition and both Cdk2 and Cyclin A associate with DNA in the initiation complex during replication. The Rb protein has been identified as a substrate for Cdk2-Cyclin E and/or Cdk2-Cyclin A in vivo. This observation is supported by further evidence which shows that Cdk2 is activated and specifically localized to the nucleus during late G1, S phase, and G2.
Molecular Weight 33 kDa
Research Area Cancer, Cell Cycle, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, Cell Division Cycle

Application Details

Product Details anti-CDK2 Antibody Target Details CDK2 Handling References for anti-CDK2 Antibody (ABIN967777) Images back to top
Application Notes Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.
Comment

Related Products: ABIN967389, ABIN968537

Restrictions For Research Use only

Handling

Product Details anti-CDK2 Antibody Target Details CDK2 Application Details References for anti-CDK2 Antibody (ABIN967777) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-CDK2 Antibody (ABIN967777)

Product Details anti-CDK2 Antibody Target Details CDK2 Application Details Handling Images back to top
Product cited in:

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, 2002

Mohapatra, Agrawal, Pledger: "p27Kip1 regulates T cell proliferation." in: The Journal of biological chemistry, Vol. 276, Issue 24, pp. 21976-83, 2001

Porter, Zhang, Danes, McGahren, Harwell, Faruki, Keyomarsi: "Tumor-specific proteolytic processing of cyclin E generates hyperactive lower-molecular-weight forms." in: Molecular and cellular biology, Vol. 21, Issue 18, pp. 6254-69, 2001

Mal, Chattopadhyay, Ghosh, Poon, Hunter, Harter: "p21 and retinoblastoma protein control the absence of DNA replication in terminally differentiated muscle cells." in: The Journal of cell biology, Vol. 149, Issue 2, pp. 281-92, 2000

Hinds, Mittnacht, Dulic, Arnold, Reed, Weinberg: "Regulation of retinoblastoma protein functions by ectopic expression of human cyclins." in: Cell, Vol. 70, Issue 6, pp. 993-1006, 1992

Images

Product Details anti-CDK2 Antibody Target Details CDK2 Application Details Handling References for anti-CDK2 Antibody (ABIN967777) back to top
Supplier Images
Western Blotting (WB) image for anti-CDK2 antibody (Cyclin-Dependent Kinase 2) (AA 109-298) (ABIN967777) Western blot analysis of Cdk2 on a Jurkat lysate. Lane 1: 1:2500, lane 2: 1:5000, lan...
Immunofluorescence (IF) image for anti-CDK2 antibody (Cyclin-Dependent Kinase 2) (AA 109-298) (ABIN967777) Immunofluorescent staining of HeLa (ATCC CCL-2) cells. Cells were seeded in a 96 well...
 image for anti-CDK2 antibody (Cyclin-Dependent Kinase 2) (AA 109-298) (ABIN967777) anti-Cyclin-Dependent Kinase 2 (CDK2) (AA 109-298) antibody (Image 3)
Western Blotting (WB) image for anti-CDK2 antibody (Cyclin-Dependent Kinase 2) (AA 109-298) (ABIN967777) anti-Cyclin-Dependent Kinase 2 (CDK2) (AA 109-298) antibody (Image 4)