anti-Human CHUK antibody for Enzyme Immunoassay

Recommended CHUK Antibody (supplied by: Log in to see )

Antigen
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
  • ikk
  • ikk1
  • ikka
  • ikkalpha
  • CHUK
  • IKBKA
  • IKK-alpha
  • IKK1
  • IKKA
  • NFKBIKA
  • TCF16
  • AI256658
  • Chuk1
  • Fbx24
  • Fbxo24
  • Ikka
  • zgc:56539
  • conserved helix-loop-helix ubiquitous kinase
  • chuk
  • CHUK
  • Chuk
Reactivity
Human, Mouse (Murine), Rat (Rattus)
353
153
104
20
12
11
10
7
6
5
5
4
2
1
Host
Rabbit
273
83
12
1
Clonality
Polyclonal
Conjugate
This CHUK antibody is un-conjugated
15
11
11
9
8
8
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
318
151
95
77
62
48
44
29
26
19
8
5
3
3
2
2
1
1
1
Supplier
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Available images

Catalog No. ABIN265477
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1107728 EIA FACS IF WB Mouse IgG1 Log in to see 3G12H9 1
1 ABIN1107727 EIA WB Mouse IgG1 Log in to see 3G12 1
1 ABIN5554860 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN5554862 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN5554861 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1107726 EIA WB Mouse IgG1 Log in to see 7C10B6 2
1 ABIN303080 EIA IF IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN5556642 EIA IP WB Rabbit C-Term Log in to see Polyclonal

General

Antigen conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(353), (153), (104), (20), (12), (11), (10), (7), (6), (5), (5), (4), (2), (1)
Host Rabbit
(273), (83), (12), (1)
Clonality
Polyclonal
Conjugate This CHUK antibody is un-conjugated
(15), (11), (11), (9), (8), (8), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(318), (151), (95), (77), (62), (48), (44), (29), (26), (19), (8), (5), (3), (3), (2), (2), (1), (1), (1)
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Product Details anti-CHUK Antibody

Target Details CHUK Application Details Handling Images
Specificity This antibody detects endogenous levels of IKK-α protein. (region surrounding Met17)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Purity > 95 % pure by SDS-PAGE

Target Details CHUK

Product Details anti-CHUK Antibody Application Details Handling Images back to top
Antigen
Alternative Name IKKA (CHUK Antibody Abstract)
Background The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins. Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB. The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation.Synonyms: CHUK, Conserved helix-loop-helix ubiquitous kinase, I kappa-B kinase alpha, I-kappa-B kinase 1, IKK-A, IKK-alpha, IKK1, IkBKA, IkappaB kinase, Inhibitor of nuclear factor kappa-B kinase subunit alpha, NFKBIKA, Nuclear factor NF-kappa-B inhibitor kinase alpha, TCF16
Molecular Weight approx. 85 kDa
Gene ID 1147
NCBI Accession NP_001269
UniProt O15111
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-CHUK Antibody Target Details CHUK Handling Images back to top
Application Notes ELISA: 1: 10000approx. 1:20000. WB: 1:500approx. 1:1000. IHC: 1:50approx. 1:200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CHUK Antibody Target Details CHUK Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice DO NOT FREEZE!
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

Images

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling back to top
Supplier Images
 image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody (ABIN265477) anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody
 image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody (ABIN265477) anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody (Image 2)