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anti-Monkey Cyclin D1 antibody for Immunoprecipitation

Recommended Cyclin D1 Antibody (supplied by: Log in to see )

Antigen
Cyclin D1 (CCND1) Antibodies
  • ccnd1b
  • prad1
  • bcl1
  • d11s287e
  • u21b31
  • ccndbp1
  • ccnd1
  • ZHM
  • CCND1
  • BCL1
  • D11S287E
  • PRAD1
  • U21B31
  • AI327039
  • CycD1
  • Cyl-1
  • bcl-1
  • cD1
  • ccnd1a
  • cb161
  • cycd1
  • etID37810.7
  • fb52e01
  • fc45c08
  • fc83a12
  • id:ibd1198
  • wu:fb52e01
  • wu:fc45c08
  • wu:fc83a12
  • cyclin D1
  • Cyclin-D1-binding protein 1 homolog
  • cyclin Dx
  • zgc:162082
  • G1/S-specific cyclin-D1
  • ccnd1-b
  • ccnd1
  • ccndbp1
  • ccndx
  • zgc:162082
  • CCND1
  • Ccnd1
  • ccnd1-a
Epitope
AA 285-295
76
24
19
16
13
12
10
10
9
8
8
6
5
5
5
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Bat, Hamster, Human, Monkey, Mouse (Murine), Rat (Rattus)
470
241
230
44
20
19
19
16
16
15
4
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
Host
Rabbit
312
198
19
4
Clonality
Polyclonal
Conjugate
This Cyclin D1 antibody is un-conjugated
23
20
12
10
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
430
205
126
124
111
108
103
78
50
14
13
12
8
7
7
6
4
3
2
2
1
1
1
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Catalog No. ABIN211137
$ 793.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1824080 FACS IP WB Mouse IgG2a Log in to see DCS-6
1 ABIN385278 BR FACS IF IP WB Biotin Mouse IgG2a, kappa Log in to see DCS-6
1 ABIN2618272 ELISA FACS IF IHC (fro) IHC (p) IP WB Mouse IgG2a, kappa Log in to see
1 ABIN1824125 FACS IP WB Mouse IgG2a Log in to see DCS-6
1 ABIN284021 FACS IF IP IHC WB Mouse IgG1 kappa Log in to see DCS-6
1 ABIN1817862 FACS IHC (p) IP WB Rabbit IgG Log in to see Polyclonal

Similar anti-Cyclin D1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Monkey Bat Hamster
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies 4 Antibodies 5 Antibodies
Cytometry by Time of Flight (CyTOF) 4 Antibodies 2 Antibodies 2 Antibodies
Western Blotting (WB) 370 Antibodies 16 Antibodies 192 Antibodies 183 Antibodies 4 Antibodies 2 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Intracellular Staining (ICS) 1 Antibodies 1 Antibodies 1 Antibodies
Immunoprecipitation (IP) 100 Antibodies 7 Antibodies 70 Antibodies

General

Antigen Cyclin D1 (CCND1) Antibodies
Epitope AA 285-295
(76), (24), (19), (16), (13), (12), (10), (10), (9), (8), (8), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bat, Hamster, Human, Monkey, Mouse (Murine), Rat (Rattus)
(470), (241), (230), (44), (20), (19), (19), (16), (16), (15), (4), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(312), (198), (19), (4)
Clonality
Polyclonal
Conjugate This Cyclin D1 antibody is un-conjugated
(23), (20), (12), (10), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
(430), (205), (126), (124), (111), (108), (103), (78), (50), (14), (13), (12), (8), (7), (7), (6), (4), (3), (2), (2), (1), (1), (1)
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Product Details anti-Cyclin D1 Antibody

Target Details Cyclin D1 Application Details Handling Images
Specificity Recognizes p36 Cyclin D1 and cross-reacts with p34 Cyclin D2, which has a highly homologous epitope. No cross-reactivity with Cyclin A or Cyclin B. Shows reactivity with a 28kD band that can competed away by pre-incubating antibody with the peptide immunogen. Also detect a nonspecific band at 21kD. Species cross-reactivity: mouse and rat.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Bat (100%) Elephant, Panda, Bovine, Dog, Horse, Turkey, Chicken, Platypus, Lizard, Xenopus, Salmon, Pufferfish (91%) Opossum, Stickleback, Zebrafish (82%).
Purification Protein A purified
Immunogen Synthetic peptide corresponding to the 11 C-terminal amino acids (residues 285-295, sequence: CTPTDVRDVDI) of human Cyclin D1. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Bat (100%), Elephant, Panda, Bovine, Dog, Horse, Turkey, Chicken, Platypus, Lizard, Xenopus, Salmon, Pufferfish (91%), Opossum, Stickleback, Zebrafish (82%).

Type of Immunogen: Synthetic peptide
Isotype IgG

Target Details Cyclin D1

Product Details anti-Cyclin D1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CCND1 / Cyclin D1 (CCND1 Antibody Abstract)
Background Name/Gene ID: CCND1

Synonyms: CCND1, B-cell lymphoma 1 protein, BCL-1 oncogene, B-cell CLL/lymphoma 1, BCL-1, Cyclin D1, G1/S-specific cyclin-D1, U21B31, PRAD1, BCL1, D11S287E, PRAD1 oncogene
Gene ID 595
UniProt P24385
Pathways PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases

Application Details

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Handling Images back to top
Application Notes Approved: Func, ICC, IP, WB

Usage: Western Blot Analysis 2: At 0.5-2 μg/mL, this antibody detected Cyclin D in a RIPA lysates of human A431 cells, mouse 3T3 fibroblasts. Positive Antigen Control: EGF-stimulated A431 cell lysate. Use 20 μg per lane. Immunoprecipitation: 5 μg of antibody immunoprecipitated Cyclin D from a RIPA lysate of human A431 cells. An H1 kinase assay can be performed with the immunoprecipitate, as reported previously. Immunocytochemistry: At 10 μg/mL, a previous lot of antibody detected Cyclin D in cells fixed with 4 % paraformaldehyde/0.1 % Triton X-100. Western Blot Analysis EGF-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Cyclin D (2 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Restrictions For Research Use only

Handling

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.