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anti-Mouse (Murine) Cyclin D1 antibody for Immunofluorescence

Recommended Cyclin D1 Antibody (supplied by: Log in to see )

Antigen
Cyclin D1 (CCND1) Antibodies
  • AI327039
  • bcl-1
  • bcl1
  • BCL1
  • cb161
  • ccnd1
  • CCND1
  • ccnd1a
  • ccnd1b
  • ccndbp1
  • cD1
  • cycd1
  • CycD1
  • Cyl-1
  • d11s287e
  • D11S287E
  • etID37810.7
  • fb52e01
  • fc45c08
  • fc83a12
  • id:ibd1198
  • prad1
  • PRAD1
  • u21b31
  • U21B31
  • wu:fb52e01
  • wu:fc45c08
  • wu:fc83a12
  • ZHM
Epitope
AA 68-97
77
22
22
15
13
12
10
10
9
9
8
7
6
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
454
184
176
44
16
15
13
11
8
5
4
3
2
2
2
2
1
1
1
1
Host
Rabbit
307
192
3
Clonality
Polyclonal
Conjugate
This Cyclin D1 antibody is un-conjugated
22
19
11
9
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
400
193
140
122
110
106
99
75
68
13
12
8
8
7
6
3
2
2
2
1
1
Supplier
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Catalog No. ABIN3030588
$ 411.13
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011096579 ABIN1106872 IHC (fro) IF IHC (p) WB Mouse IgG2a Log in to see CY-D1 3
0.0011096579 ABIN389146 IF IHC (p) WB Rabbit Ig AA 68-97, Ser90 Log in to see Polyclonal 3
0.0011096579 ABIN181311 IF IP WB Rabbit C-Term Log in to see Polyclonal 3
0.0011096579 ABIN200706 IF IP IHC WB Mouse IgG1 Log in to see
0.0011096579 ABIN1732740 IF IHC (p) ELISA WB Rabbit IgG Ser90 Log in to see Polyclonal
0.0011096579 ABIN4301541 FACS ICC IF IHC (p) IP WB Mouse IgG2a kappa Log in to see SPM587
0.0011096579 ABIN4301540 FACS ICC IF IHC (p) IP WB Mouse IgG2a kappa Log in to see SPM587
0.0011096579 ABIN4301539 FACS ICC IF IHC (p) IP WB Mouse IgG2a kappa Log in to see SPM587
0.0011096579 ABIN2736604 IF IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0011096579 ABIN4301563 FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG2a kappa Log in to see SPM587
0.0011096579 ABIN4301564 FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG2a kappa Log in to see SPM587
0.0011096579 ABIN4301570 FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG2a kappa Log in to see SPM587
0.0011096579 ABIN4301566 FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG2a kappa Log in to see SPM587
0.0011096579 ABIN4301568 FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG2a kappa Log in to see SPM587
0.0011096579 ABIN4301573 FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG2a kappa Log in to see SPM587
0.0011096579 ABIN4301575 FACS ICC IF IHC (fro) IHC (p) IP WB PE Mouse IgG2a kappa Log in to see SPM587
0.0011096579 ABIN4301577 FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG2a kappa Log in to see SPM587
0.0011096579 ABIN4301574 FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG2a kappa Log in to see SPM587
0.0011096579 ABIN4301576 FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 550 Mouse IgG2a kappa Log in to see SPM587
0.0011096579 ABIN4301567 FACS ICC IF IHC (fro) IHC (p) IP WB Allophycocyanin Mouse IgG2a kappa Log in to see SPM587

Top referenced anti-Cyclin D1 antibody for Immunofluorescence

Similar anti-Cyclin D1 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 61 Antibodies 167 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 65 Antibodies
Functional Studies (Func) 2 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 56 Antibodies
Immunofluorescence (IF) 62 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 12 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 45 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 42 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 64 Antibodies
Immunoprecipitation (IP) 64 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Western Blotting (WB) 153 Antibodies
Blocking Reagent (BR) 2 Antibodies
Dot Blot (DB) 3 Antibodies

General

Antigen Cyclin D1 (CCND1) Antibodies
Epitope AA 68-97
(77), (22), (22), (15), (13), (12), (10), (10), (9), (9), (8), (7), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(454), (184), (176), (44), (16), (15), (13), (11), (8), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(307), (192), (3)
Clonality
Polyclonal
Conjugate This Cyclin D1 antibody is un-conjugated
(22), (19), (11), (9), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(400), (193), (140), (122), (110), (106), (99), (75), (68), (13), (12), (8), (8), (7), (6), (3), (2), (2), (2), (1), (1)
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Product Details anti-Cyclin D1 Antibody

Target Details Cyclin D1 Application Details Handling Images
Cross-Reactivity (Details) Expected species reactivity: Bovine
Purification Antigen affinity purified
Immunogen A portion of amino acids 68-97 from the human protein was used as the immunogen for this Cyclin D1 antibody.
Isotype Ig Fraction

Target Details Cyclin D1

Product Details anti-Cyclin D1 Antibody Application Details Handling Images back to top
Antigen
Background CCND1 is the regulatory component of the Cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. CyclinD-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. [UniProt]
UniProt P24385
Pathways PI3K-Akt Signaling, Cell Division Cycle

Application Details

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Handling Images back to top
Application Notes Titration of the Cyclin D1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50
Restrictions For Research Use only

Handling

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Images back to top
Format Liquid
Buffer In 1X PBS, pH 7.4, with 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Aliquot the Cyclin D1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-Cyclin D1 antibody (CCND1) (AA 68-97) (ABIN3030588) Confocal immunofluorescent analysis of Cyclin D1 antibody with HeLa cells followed by...
Immunohistochemistry (IHC) image for anti-Cyclin D1 antibody (CCND1) (AA 68-97) (ABIN3030588) IHC analysis of FFPE human breast carcinoma tissue stained with Cyclin D1 antibody
Immunohistochemistry (IHC) image for anti-Cyclin D1 antibody (CCND1) (AA 68-97) (ABIN3030588) IHC analysis of FFPE human testis tissue stained with Cyclin D1 antibody
Western Blotting (WB) image for anti-Cyclin D1 antibody (CCND1) (AA 68-97) (ABIN3030588) Western blot analysis of Cyclin D1 antibody and mouse lung lysate. Predicted molecula...