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anti-Human Huntingtin antibody for Enzyme Immunoassay

Recommended Huntingtin Antibody (supplied by: Log in to see )

Antigen
Huntingtin (HTT) Antibodies
  • AI256365
  • C430023I11Rik
  • CG9995
  • dHtt
  • dhtt
  • Dmel\\CG9995
  • HD
  • hd
  • Hd
  • Hdh
  • Hsap\\HD
  • htt
  • HTT
  • Htt
  • huntington
  • it15
  • IT15
  • SLC6A4
  • ZHD
Epitope
AA 416-424, pSer421
14
12
12
9
7
7
6
5
5
4
4
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
155
76
42
10
2
Host
Rabbit
85
73
3
1
Clonality
Polyclonal
Conjugate
This Huntingtin antibody is un-conjugated
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
127
74
52
24
24
22
18
18
11
8
3
2
2
Supplier
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Catalog No. ABIN117993
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN303085 EIA IHC (p) WB Rabbit AA 416-424, pSer421 Log in to see Polyclonal
0.0008867729 ABIN781873 EIA IHC (p) WB Mouse IgG2a AA 81-191 Log in to see 3F1

Top referenced anti-Huntingtin antibody for Enzyme Immunoassay

Similar anti-Huntingtin Antibodies

Application / Reactivity Human
Dot Blot (DB) 3 Antibodies
ELISA 72 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Immunochromatography (IC) 8 Antibodies
Immunocytochemistry (ICC) 16 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 49 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 21 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies
Immunoprecipitation (IP) 24 Antibodies
Western Blotting (WB) 123 Antibodies

General

Antigen Huntingtin (HTT) Antibodies
Epitope AA 416-424, pSer421
(14), (12), (12), (9), (7), (7), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(155), (76), (42), (10), (2)
Host Rabbit
(85), (73), (3), (1)
Clonality
Polyclonal
Conjugate This Huntingtin antibody is un-conjugated
(2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(127), (74), (52), (24), (24), (22), (18), (18), (11), (8), (3), (2), (2)
Pubmed 2 references available
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Product Details anti-Huntingtin Antibody

Target Details Huntingtin Application Details Handling References for anti-Huntingtin Antibody (ABIN117993) Images
Sequence Human Huntingtin protein, 3144 aa, predicted MW 347.7 kDa
Specificity This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human Huntingtin protein at the pS421 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Huntingtin homologues from chimpanzee, pig and chicken. Cross reactivity with Huntingtin protein homologues from mouse and rat may also occur as sequence homology varies by one amino acids residues in this sequence. Reactivity with Huntington protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.
Purification Immunoaffinity chromatography.
Immunogen Synthetic peptide corresponding aa 416-424 of Human huntingtin protein.

Target Details Huntingtin

Product Details anti-Huntingtin Antibody Application Details Handling References for anti-Huntingtin Antibody (ABIN117993) Images back to top
Antigen
Alternative Name Huntingtin (HTT Antibody Abstract)
Background Huntingtin (also known as Huntington's disease protein and HD protein) is the protein product of a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This may be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. The huntingtin gene locus is large, spanning 180 kb and consisting of 67 exons. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. Normal huntingtin protein shows a cytoplasmic localization. This protein is widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.Synonyms: HD, HTT, Huntington Disease Protein, IT15
Gene ID 3064
NCBI Accession NP_002102
UniProt P42858
Research Area Neurology, Virology
Pathways PI3K-Akt Signaling

Application Details

Product Details anti-Huntingtin Antibody Target Details Huntingtin Handling References for anti-Huntingtin Antibody (ABIN117993) Images back to top
Application Notes Western blotting (1: 500 - 1: 3,000). ELISA (1: 10,000 - 1: 40,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details References for anti-Huntingtin Antibody (ABIN117993) Images back to top
Concentration 0.8 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 2-8 °C.

References for anti-Huntingtin Antibody (ABIN117993)

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Handling Images back to top
Background publications

Lee, Yoshihara, Littleton: "Cytoplasmic aggregates trap polyglutamine-containing proteins and block axonal transport in a Drosophila model of Huntington's disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 9, pp. 3224-9, 2004

Zainelli, Ross, Troncoso, Fitzgerald, Muma: "Calmodulin regulates transglutaminase 2 cross-linking of huntingtin." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 24, Issue 8, pp. 1954-61, 2004

Images

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Handling References for anti-Huntingtin Antibody (ABIN117993) back to top
Supplier Images
Western Blotting (WB) image for anti-Huntingtin antibody (HTT) (AA 416-424) (ABIN117993) Western blot analysis is shown using anti-Huntingtin pS421 antibody to detect endogen...