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|Antigen||Huntingtin (HTT) Antibodies|
|Epitope||AA 416-424, pSer421 Alternatives|
|Conjugate||This Huntingtin antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-Huntingtin AntibodyTarget Details Huntingtin Application Details Handling Images
|Sequence||Human Huntingtin protein, 3144 aa, predicted MW 347.7 kDa|
|Specificity||This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human Huntingtin protein at the pS421 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Huntingtin homologues from chimpanzee, pig and chicken. Cross reactivity with Huntingtin protein homologues from mouse and rat may also occur as sequence homology varies by one amino acids residues in this sequence. Reactivity with Huntington protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.|
|Immunogen||Synthetic peptide corresponding aa 416-424 of Human huntingtin protein.|
Target Details HuntingtinProduct Details anti-Huntingtin Antibody Application Details Handling Images back to top
|Alternative Name||Huntingtin (HTT Antibody Abstract)|
|Background||Huntingtin (also known as Huntington's disease protein and HD protein) is the protein product of a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This may be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. The huntingtin gene locus is large, spanning 180 kb and consisting of 67 exons. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. Normal huntingtin protein shows a cytoplasmic localization. This protein is widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.Synonyms: HD, HTT, Huntington Disease Protein, IT15|
|Research Area||Neurology, Virology|
|Pathways||PI3K-Akt Signaling, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport|
Application DetailsProduct Details anti-Huntingtin Antibody Target Details Huntingtin Handling Images back to top
Western blotting (1: 500 - 1: 3,000). ELISA (1: 10,000 - 1: 40,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Images back to top
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 2-8 °C.|