anti-Human Huntingtin antibody for Enzyme Immunoassay

Recommended Huntingtin Antibody (supplied by: Log in to see )

Antigen
Huntingtin (HTT) Antibodies
  • HTT
  • hd
  • ZHD
  • CG9995
  • Dmel\\CG9995
  • HD
  • Hsap\\HD
  • Htt
  • dHtt
  • dhtt
  • SLC6A4
  • huntington
  • it15
  • htt
  • IT15
  • AI256365
  • C430023I11Rik
  • Hd
  • Hdh
  • solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
  • huntingtin
  • SLC6A4
  • LOC100216476
  • htt
  • Htt
  • HTT
  • HDH
Epitope
AA 416-424, pSer421
14
12
10
8
8
7
5
5
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
154
103
62
13
6
2
2
2
1
1
1
Host
Rabbit
81
78
3
1
Clonality
Polyclonal
Conjugate
This Huntingtin antibody is un-conjugated
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
133
76
74
37
26
25
24
17
13
8
3
3
2
1
Supplier
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Catalog No. ABIN5556689
$ 411.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN303085 EIA IHC (p) WB Rabbit AA 416-424, pSer421 Log in to see Polyclonal
1 ABIN781873 EIA IHC (p) WB Mouse IgG2a AA 81-191 Log in to see 3F1
1 ABIN5540398 EIA ICC IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal

General

Antigen Huntingtin (HTT) Antibodies
Epitope AA 416-424, pSer421
(14), (12), (10), (8), (8), (7), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(154), (103), (62), (13), (6), (2), (2), (2), (1), (1), (1)
Host Rabbit
(81), (78), (3), (1)
Clonality
Polyclonal
Conjugate This Huntingtin antibody is un-conjugated
(2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(133), (76), (74), (37), (26), (25), (24), (17), (13), (8), (3), (3), (2), (1)
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Product Details anti-Huntingtin Antibody

Target Details Huntingtin Application Details Handling Images
Sequence Human Huntingtin protein, 3144 aa, predicted MW 347.7 kDa
Specificity This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human Huntingtin protein at the pS421 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Huntingtin homologues from chimpanzee, pig and chicken. Cross reactivity with Huntingtin protein homologues from mouse and rat may also occur as sequence homology varies by one amino acids residues in this sequence. Reactivity with Huntington protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.
Purification Immunoaffinity chromatography.
Immunogen Synthetic peptide corresponding aa 416-424 of Human huntingtin protein.

Target Details Huntingtin

Product Details anti-Huntingtin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Huntingtin (HTT Antibody Abstract)
Background Huntingtin (also known as Huntington's disease protein and HD protein) is the protein product of a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This may be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. The huntingtin gene locus is large, spanning 180 kb and consisting of 67 exons. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. Normal huntingtin protein shows a cytoplasmic localization. This protein is widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.Synonyms: HD, HTT, Huntington Disease Protein, IT15
Gene ID 3064
NCBI Accession NP_002102
UniProt P42858
Research Area Neurology, Virology
Pathways PI3K-Akt Signaling, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport

Application Details

Product Details anti-Huntingtin Antibody Target Details Huntingtin Handling Images back to top
Application Notes Western blotting (1: 500 - 1: 3,000). ELISA (1: 10,000 - 1: 40,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Images back to top
Concentration 0.8 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 2-8 °C.