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anti-Human Huntingtin antibody for Fluorescence Microscopy

Recommended Huntingtin Antibody (supplied by: Log in to see )

Antigen
Huntingtin (HTT) Antibodies
  • AI256365
  • C430023I11Rik
  • CG9995
  • dHtt
  • dhtt
  • Dmel\\CG9995
  • HD
  • hd
  • Hd
  • Hdh
  • Hsap\\HD
  • htt
  • HTT
  • Htt
  • huntington
  • it15
  • IT15
  • SLC6A4
  • ZHD
Epitope
N-Term
13
12
12
7
6
6
5
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
124
46
34
7
Host
Rabbit
66
61
2
1
Clonality
Polyclonal
Conjugate
This Huntingtin antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Fluorescence Microscopy (FM), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
96
65
41
21
20
18
11
11
11
8
3
3
1
Supplier
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Catalog No. ABIN1607890
$ 369.00
Plus shipping costs $45.00 and $21.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008676689 ABIN1607891 FM IHC ELISA WB Rabbit C-Term Log in to see Polyclonal

Similar anti-Huntingtin Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 15 Antibodies 19 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies 2 Antibodies
Immunochromatography (IC) 1 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 5 Antibodies
Immunofluorescence (IF) 2 Antibodies 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 17 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 22 Antibodies

General

Antigen Huntingtin (HTT) Antibodies
Epitope N-Term
(13), (12), (12), (7), (6), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(124), (46), (34), (7)
Host Rabbit
(66), (61), (2), (1)
Clonality
Polyclonal
Conjugate This Huntingtin antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Fluorescence Microscopy (FM), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(96), (65), (41), (21), (20), (18), (11), (11), (11), (8), (3), (3), (1)
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Product Details anti-Huntingtin Antibody

Target Details Huntingtin Application Details Handling Images
Cross-Reactivity (Details) Cross reactivity with HTT from other sources has not been determined.
Purity Anti-Huntington was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards HTT. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, and Rat based on 100 % sequence homology.
Endotoxin Level Low Endotoxin : No
Immunogen Huntington affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the near N-terminus of human Huntington.
Immunogen Type: Peptide

Target Details Huntingtin

Product Details anti-Huntingtin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Huntington (HTT Antibody Abstract)
Background Huntington (also known as Huntington's disease protein, Htt and HD protein) is the protein product of a disease gene linked to Huntington's disease, a neuro-degenerative disorder characterized by loss of striatal neurons. This may be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product (see partial protein sequence below). The huntingtin gene locus is large, spanning 180 kb and consisting of 67 exons. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. Normal huntingtin protein shows a cytoplasmic localization. This protein is widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation. Anti-Huntingtin antibodies are ideal for researchers interested in Apoptosis, Autophagy, Cytoskeleton, Neurodegeneration, Neuroscience, and Neuronal Cell Markers research. Synonyms: HD protein, HTT, huntingtin, huntingtin (Huntington disease), IT15HD, Huntington disease protein
Gene ID 3064
NCBI Accession NP_002102
UniProt P42858
Pathways PI3K-Akt Signaling

Application Details

Product Details anti-Huntingtin Antibody Target Details Huntingtin Handling Images back to top
Application Notes Anti-Huntington antibody is useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~350 kDa corresponding to the appropriate cell lysate or extract.
ELISA Dilution: 1:20.000 - 1:60.000
Immunohistochemistry Dilution: 1:100-1:500
IF Microscopy Dilution: 1:100-1:500
Western Blot Dilution: 0.1 µg/mL
Restrictions For Research Use only

Handling

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
Storage 4 °C/-20 °C
Storage Comment Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date Expiration date is six (6) months from date of opening.