anti-Cow (Bovine) MAP3K8 antibody for Western Blotting

Recommended MAP3K8 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) Antibodies
  • MAP3K8
  • COT
  • EST
  • ESTF
  • MEKK8
  • TPL2
  • Tpl-2
  • c-COT
  • Cot
  • Cot/Tpl2
  • Est
  • Estf
  • Tpl2
  • D17TPL2
  • mitogen-activated protein kinase kinase kinase 8
  • MAP3K8
  • map3k8
  • Map3k8
Epitope
N-Term
26
16
15
10
5
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Horse (Equine), Human, Mouse (Murine)
100
48
46
10
8
4
3
3
2
1
1
Host
Rabbit
96
4
Clonality
Polyclonal
Conjugate
This MAP3K8 antibody is un-conjugated
5
5
4
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
58
39
32
30
15
10
9
3
3
3
2
2
Options
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Catalog No. ABIN2779366
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1581392 ABIN2779365 WB Rabbit C-Term Log in to see Polyclonal 1
3.1581392 ABIN2707193 IC IF IHC WB Rabbit pSer290 Log in to see Polyclonal
1 ABIN202595 WB Rabbit IgG AA 396-445 Log in to see Polyclonal
1 ABIN467660 WB Rabbit AA 35-84 Log in to see Polyclonal
1 ABIN3198352 IC IF IHC WB Rabbit Log in to see Polyclonal
1 ABIN2625110 ICC IF IHC (p) WB Rabbit pThr290 Log in to see Polyclonal
1 ABIN2972689 IF/ICC IHC WB Rabbit Center Log in to see Polyclonal
1 ABIN2994921 IF/ICC IHC WB Rabbit pThr290 Log in to see Polyclonal

Top referenced anti-MAP3K8 antibody for Western Blotting

Similar anti-MAP3K8 Antibodies

Application / Reactivity Cow (Bovine) Human Mouse (Murine) Horse (Equine)
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 2 Antibodies 2 Antibodies
Western Blotting (WB) 9 Antibodies 59 Antibodies 27 Antibodies 4 Antibodies
Immunoprecipitation (IP) 3 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 30 Antibodies 17 Antibodies 3 Antibodies
Immunohistochemistry (IHC) 32 Antibodies 15 Antibodies 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 15 Antibodies 15 Antibodies
Immunofluorescence (IF) 9 Antibodies 9 Antibodies 3 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) Antibodies
Epitope N-Term
(26), (16), (15), (10), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Horse (Equine), Human, Mouse (Murine)
(100), (48), (46), (10), (8), (4), (3), (3), (2), (1), (1)
Host Rabbit
(96), (4)
Clonality
Polyclonal
Conjugate This MAP3K8 antibody is un-conjugated
(5), (5), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(58), (39), (32), (30), (15), (10), (9), (3), (3), (3), (2), (2)
Pubmed 1 reference available
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Product Details anti-MAP3K8 Antibody

Target Details MAP3K8 Application Details Handling ProductDetails: References for anti-MAP3K8 antibody (ABIN2779366) Images
Blocking Peptide Blocking peptide for this product available: ABIN979781
Sequence ASEEPAVYEP SLMTMCQDSN QNDERSKSLL LSGQEVPWLS SVRYGTVEDL
Predicted Reactivity Cow: 75%, Horse: 87%, Human: 100%, Mouse: 85%
Characteristics This is a rabbit polyclonal antibody against MAP3K8. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human MAP3K8

Target Details MAP3K8

Product Details anti-MAP3K8 Antibody Application Details Handling ProductDetails: References for anti-MAP3K8 antibody (ABIN2779366) Images back to top
Antigen
Alternative Name MAP3K8 (MAP3K8 Antibody Abstract)
Background MAP3K8 is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways. This kinase was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This kinase was
Alias Symbols: COT, EST, ESTF, FLJ10486, TPL2, Tpl-2, c-COT, MEKK8
Protein Interaction Partner: RELA, MAP2K1, RIPK1, NFKB1, ACY3, STIP1, HSP90AA1, TAB2, TRAF6, TRAF2, CD40, TNIP2, Nedd4, MAP3K14, MAP2K4, BMPR2, CHUK, REL, AKT1, NFKB2, KSR2,
Protein Size: 467
Molecular Weight 53 kDa
Gene ID 1326
NCBI Accession NM_005204, NP_005195
UniProt P41279
Research Area Cancer
Pathways PI3K-Akt Signaling, TCR Signaling

Application Details

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Handling ProductDetails: References for anti-MAP3K8 antibody (ABIN2779366) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 467 AA

Restrictions For Research Use only

Handling

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details ProductDetails: References for anti-MAP3K8 antibody (ABIN2779366) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-MAP3K8 antibody (ABIN2779366)

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details Handling Images back to top
Background publications

Wittwer, Schmitz: "NIK and Cot cooperate to trigger NF-kappaB p65 phosphorylation." in: Biochemical and biophysical research communications, Vol. 371, Issue 2, pp. 294-7, 2008

Images

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details Handling ProductDetails: References for anti-MAP3K8 antibody (ABIN2779366) back to top
Supplier Images
Western Blotting (WB) image for anti-MAP3K8 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 8) (N-Term) (ABIN2779366) WB Suggested Anti-MAP3K8 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positi...