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anti-Dog (Canine) MAP3K8 antibody for Western Blotting

Recommended MAP3K8 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) Antibodies
  • c-COT
  • Cot
  • COT
  • Cot/Tpl2
  • D17TPL2
  • Est
  • EST
  • ESTF
  • Estf
  • MAP3K8
  • MEKK8
  • Tpl-2
  • TPL2
  • Tpl2
Epitope
C-Term
28
16
13
9
7
6
4
4
3
2
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
93
37
34
4
4
1
1
Host
Rabbit
89
4
Clonality
Polyclonal
Conjugate
This MAP3K8 antibody is un-conjugated
5
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
49
41
32
23
13
11
8
5
3
2
Supplier
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Catalog No. ABIN2779365
$ 229.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.11933539 ABIN2460190 ELISA WB Rabbit Log in to see Polyclonal

Top referenced anti-MAP3K8 antibody for Western Blotting

  • Chicken Polyclonal MAP3K8 Primary Antibody for WB - ABIN2779365 (1 Publications): Jia, Quinn, Bump, Clark, Clabbers, Hardman, Gagnon, Kamens, Tomlinson, Wishart, Allen: Purification and kinetic characterization of recombinant human mitogen-activated protein kinase kinase kinase COT and the complexes with its cellular partner NF-kappa B1 p105. in Archives of biochemistry and biophysics 2005 (PubMed)

Similar anti-MAP3K8 Antibodies

Application / Reactivity Chicken Cow (Bovine) Dog (Canine) Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 1 Antibodies 4 Antibodies 2 Antibodies 50 Antibodies 20 Antibodies 17 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies 8 Antibodies 6 Antibodies
Immunohistochemistry (IHC) 32 Antibodies 14 Antibodies 14 Antibodies 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies 13 Antibodies 13 Antibodies
Immunofluorescence (IF) 8 Antibodies 7 Antibodies 4 Antibodies 3 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) Antibodies
Epitope C-Term
(28), (16), (13), (9), (7), (6), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(93), (37), (34), (4), (4), (1), (1)
Host Rabbit
(89), (4)
Clonality
Polyclonal
Conjugate This MAP3K8 antibody is un-conjugated
(5), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(49), (41), (32), (23), (13), (11), (8), (5), (3), (2)
Pubmed 1 reference available
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Product Details anti-MAP3K8 Antibody

Target Details MAP3K8 Application Details Handling References for anti-MAP3K8 Antibody (ABIN2779365) Images
Sequence PRCQSLDSAL LERKRLLSRK ELELPENIAD SSCTGSTEES EMLKRQRSLY
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Horse : 100 %, Human : 100 %, Rat : 100 %, Mouse : 90 %, Chicken : 80 %, Rabbit : 70 %
Characteristics This is a rabbit polyclonal antibody against MAP3K8. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human MAP3K8

Target Details MAP3K8

Product Details anti-MAP3K8 Antibody Application Details Handling References for anti-MAP3K8 Antibody (ABIN2779365) Images back to top
Antigen
Alternative Name MAP3K8 (MAP3K8 Antibody Abstract)
Background MAP3K8 is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways. This kinase was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This kinase was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. Studies of a similar gene in rat suggested the direct involvement of this kinase in the proteolysis of NF-kappaB1,p105 (NFKB1). This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity.
Molecular Weight 53 kDa
Gene ID 1326
NCBI Accession NP_005195, NM_005204
UniProt P41279
Research Area Cancer
Pathways PI3K-Akt Signaling, TCR Signaling

Application Details

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Handling References for anti-MAP3K8 Antibody (ABIN2779365) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 467 AA

Restrictions For Research Use only

Handling

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details References for anti-MAP3K8 Antibody (ABIN2779365) Images back to top
Format Liquid
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-MAP3K8 Antibody (ABIN2779365)

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details Handling Images back to top
Background publications

Jia, Quinn, Bump, Clark, Clabbers, Hardman, Gagnon, Kamens, Tomlinson, Wishart, Allen: "Purification and kinetic characterization of recombinant human mitogen-activated protein kinase kinase kinase COT and the complexes with its cellular partner NF-kappa B1 p105." in: Archives of biochemistry and biophysics, Vol. 441, Issue 1, pp. 64-74, 2005

Images

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details Handling References for anti-MAP3K8 Antibody (ABIN2779365) back to top
Supplier Images
Western Blotting (WB) image for anti-MAP3K8 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 8) (C-Term) (ABIN2779365) WB Suggested Anti-MAP3K8 Antibody Titration: 2.5ug/ml
ELISA Titer: 1...