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|Antigen||Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) Antibodies|
|Conjugate||This MAP3K8 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-MAP3K8 AntibodyTarget Details MAP3K8 Application Details Handling ProductDetails: References for anti-MAP3K8 antibody (ABIN360298) Images
|Specificity||This antibody reacts to COT (MAP3K8/MEKK8).|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human MEKK8.|
Target Details MAP3K8Product Details anti-MAP3K8 Antibody Application Details Handling ProductDetails: References for anti-MAP3K8 antibody (ABIN360298) Images back to top
|Alternative Name||MAP3K8 / TPL-2 (MAP3K8 Antibody Abstract)|
|Background||Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MEKK8 is able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. The protein appears to play an important role in the cell cycle. This cytoplasmic protein is expressed in several normal tissues and human tumor-derived cell lines. The 58 kDa form is activated specifically during the S and G2/M phases of the cell cycle. The longer form undergoes phosphorylation on Ser residues mainly, and the shorter form on both Ser and Thr residues.Synonyms: COT, COT proto-oncogene serine/threonine-protein kinase, Cancer Osaka thyroid oncogene, ESTF, MAPK, Mitogen-activated protein kinase kinase kinase 8, Tumor progression locus 2, Tumor progression locus 2|
|Gene ID||1326, 9606|
|Pathways||PI3K-Akt Signaling, TCR Signaling|
Application DetailsProduct Details anti-MAP3K8 Antibody Target Details MAP3K8 Handling ProductDetails: References for anti-MAP3K8 antibody (ABIN360298) Images back to top
ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details ProductDetails: References for anti-MAP3K8 antibody (ABIN360298) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
ProductDetails: References for anti-MAP3K8 antibody (ABIN360298)Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details Handling Images back to top
Sánchez-Góngora, Lisbona, de Gregorio, Ballester, Calvo, Pérez-Jurado, Alemany: "COT kinase proto-oncogene expression in T cells: implication of the JNK/SAPK signal transduction pathway in COT promoter activation." in: The Journal of biological chemistry, Vol. 275, Issue 40, pp. 31379-86, 2000
Shlomchik, Zharhary, Saunders, Camper, Weigert: "A rheumatoid factor transgenic mouse model of autoantibody regulation." in: International immunology, Vol. 5, Issue 10, pp. 1329-41, 1994
Aoki, Hamada, Sugimoto, Sumida, Akiyama, Toyoshima: "The human cot proto-oncogene encodes two protein serine/threonine kinases with different transforming activities by alternative initiation of translation." in: The Journal of biological chemistry, Vol. 268, Issue 30, pp. 22723-32, 1993
Miyoshi, Higashi, Mukai, Ohuchi, Kakunaga: "Structure and transforming potential of the human cot oncogene encoding a putative protein kinase." in: Molecular and cellular biology, Vol. 11, Issue 8, pp. 4088-96, 1991
Aoki, Akiyama, Miyoshi, Toyoshima: "Identification and characterization of protein products of the cot oncogene with serine kinase activity." in: Oncogene, Vol. 6, Issue 9, pp. 1515-9, 1991
ImagesProduct Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details Handling ProductDetails: References for anti-MAP3K8 antibody (ABIN360298) back to top