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anti-Human MAP3K8 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended MAP3K8 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) Antibodies
  • c-COT
  • Cot
  • COT
  • Cot/Tpl2
  • D17TPL2
  • Est
  • EST
  • ESTF
  • Estf
  • MAP3K8
  • MEKK8
  • Tpl-2
  • TPL2
  • Tpl2
Epitope
C-Term
28
16
13
9
7
6
4
4
3
2
1
1
1
1
1
1
1
1
Reactivity
Human
93
38
35
5
4
2
1
1
Host
Rabbit
89
4
Clonality
Polyclonal
Conjugate
This MAP3K8 antibody is un-conjugated
5
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
49
41
32
22
13
11
8
5
2
2
Supplier
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Catalog No. ABIN360298
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089850504 ABIN360360 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 5
0.00089850504 ABIN265369 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.00089850504 ABIN392472 IHC (p) WB Rabbit Ig AA 368-397, C-Term Log in to see Polyclonal
0.00089850504 ABIN2855464 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.00089850504 ABIN2855466 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.00089850504 ABIN392412 IHC (p) WB Rabbit Ig AA 414-445, C-Term Log in to see Polyclonal
0.00089850504 ABIN1049035 IHC (p) Rabbit N-Term Log in to see Polyclonal
0.00089850504 ABIN1049034 IHC (p) Rabbit C-Term Log in to see Polyclonal
0.00089850504 ABIN746573 IF (p) IHC (p) WB Rabbit IgG AA 370-420, pSer400 Log in to see Polyclonal
0.00089850504 ABIN746588 IF (p) IHC (p) Rabbit IgG AA 270-320, pThr290 Log in to see Polyclonal
0.00089850504 ABIN2888594 IF IHC (p) ELISA WB Rabbit IgG AA 256-305 Log in to see Polyclonal
0.00089850504 ABIN701275 IF (p) IHC (p) WB Rabbit IgG AA 140-190 Log in to see Polyclonal
0.00089850504 ABIN2889108 IHC (p) ELISA WB Rabbit IgG AA 366-415 Log in to see Polyclonal
0.00089850504 ABIN701277 IHC (p) WB Biotin Rabbit IgG AA 140-190 Log in to see Polyclonal
0.00089850504 ABIN701284 IHC (p) WB HRP Rabbit IgG AA 140-190 Log in to see Polyclonal
0.00089850504 ABIN1736725 IHC (p) Rabbit IgG C-Term Log in to see Polyclonal
0.00089850504 ABIN1736726 IHC (p) Rabbit IgG N-Term Log in to see Polyclonal
0.00089850504 ABIN1841365 IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.00089850504 ABIN1841477 IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.00089850504 ABIN4218936 IHC (p) Rabbit pSer400 Log in to see Polyclonal

Similar anti-MAP3K8 Antibodies

Application / Reactivity Human
Dot Blot (DB) 11 Antibodies
ELISA 41 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 32 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies
Western Blotting (WB) 50 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) Antibodies
Epitope C-Term
(28), (16), (13), (9), (7), (6), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(93), (38), (35), (5), (4), (2), (1), (1)
Host Rabbit
(89), (4)
Clonality
Polyclonal
Conjugate This MAP3K8 antibody is un-conjugated
(5), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(49), (41), (32), (22), (13), (11), (8), (5), (2), (2)
Pubmed 5 references available
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Product Details anti-MAP3K8 Antibody

Target Details MAP3K8 Application Details Handling References for anti-MAP3K8 Antibody (ABIN360298) Images
Specificity This antibody reacts to COT (MAP3K8/MEKK8).
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human MEKK8.
Isotype Ig

Target Details MAP3K8

Product Details anti-MAP3K8 Antibody Application Details Handling References for anti-MAP3K8 Antibody (ABIN360298) Images back to top
Antigen
Alternative Name MAP3K8 / TPL-2 (MAP3K8 Antibody Abstract)
Background Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MEKK8 is able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. The protein appears to play an important role in the cell cycle. This cytoplasmic protein is expressed in several normal tissues and human tumor-derived cell lines. The 58 kDa form is activated specifically during the S and G2/M phases of the cell cycle. The longer form undergoes phosphorylation on Ser residues mainly, and the shorter form on both Ser and Thr residues.Synonyms: COT, COT proto-oncogene serine/threonine-protein kinase, Cancer Osaka thyroid oncogene, ESTF, MAPK, Mitogen-activated protein kinase kinase kinase 8, Tumor progression locus 2, Tumor progression locus 2
Gene ID 1326, 9606
UniProt P41279
Pathways PI3K-Akt Signaling, TCR Signaling

Application Details

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Handling References for anti-MAP3K8 Antibody (ABIN360298) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details References for anti-MAP3K8 Antibody (ABIN360298) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-MAP3K8 Antibody (ABIN360298)

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details Handling Images back to top
Background publications

Sánchez-Góngora, Lisbona, de Gregorio, Ballester, Calvo, Pérez-Jurado, Alemany: "COT kinase proto-oncogene expression in T cells: implication of the JNK/SAPK signal transduction pathway in COT promoter activation." in: The Journal of biological chemistry, Vol. 275, Issue 40, pp. 31379-86, 2000

Shlomchik, Zharhary, Saunders, Camper, Weigert: "A rheumatoid factor transgenic mouse model of autoantibody regulation." in: International immunology, Vol. 5, Issue 10, pp. 1329-41, 1994

Aoki, Hamada, Sugimoto, Sumida, Akiyama, Toyoshima: "The human cot proto-oncogene encodes two protein serine/threonine kinases with different transforming activities by alternative initiation of translation." in: The Journal of biological chemistry, Vol. 268, Issue 30, pp. 22723-32, 1993

Miyoshi, Higashi, Mukai, Ohuchi, Kakunaga: "Structure and transforming potential of the human cot oncogene encoding a putative protein kinase." in: Molecular and cellular biology, Vol. 11, Issue 8, pp. 4088-96, 1991

Aoki, Akiyama, Miyoshi, Toyoshima: "Identification and characterization of protein products of the cot oncogene with serine kinase activity." in: Oncogene, Vol. 6, Issue 9, pp. 1515-9, 1991

Images

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details Handling References for anti-MAP3K8 Antibody (ABIN360298) back to top
Supplier Images
 image for anti-MAP3K8 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 8) (C-Term) (ABIN360298) anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) (C-Term) antibody
 image for anti-MAP3K8 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 8) (C-Term) (ABIN360298) anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) (C-Term) antibody (Image 2)