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anti-Mouse (Murine) MAP3K8 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended MAP3K8 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) Antibodies
  • c-COT
  • Cot
  • COT
  • Cot/Tpl2
  • D17TPL2
  • Est
  • EST
  • ESTF
  • Estf
  • MAP3K8
  • MEKK8
  • Tpl-2
  • TPL2
  • Tpl2
Reactivity
Human, Mouse (Murine), Rat (Rattus)
93
37
34
5
4
2
1
1
Host
Rabbit
89
4
Clonality
Polyclonal
Conjugate
This MAP3K8 antibody is un-conjugated
5
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
49
41
32
22
13
11
7
5
3
2
Supplier
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Catalog No. ABIN265369
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008917441 ABIN2855464 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.0008917441 ABIN2855466 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.0008917441 ABIN746573 IF (p) IHC (p) WB Rabbit IgG AA 370-420, pSer400 Log in to see Polyclonal
0.0008917441 ABIN746588 IF (p) IHC (p) Rabbit IgG AA 270-320, pThr290 Log in to see Polyclonal
0.0008917441 ABIN701275 IF (p) IHC (p) WB Rabbit IgG AA 140-190 Log in to see Polyclonal
0.0008917441 ABIN701277 IHC (p) WB Biotin Rabbit IgG AA 140-190 Log in to see Polyclonal
0.0008917441 ABIN701284 IHC (p) WB HRP Rabbit IgG AA 140-190 Log in to see Polyclonal

Similar anti-MAP3K8 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 14 Antibodies 13 Antibodies 41 Antibodies
Immunochromatography (IC) 2 Antibodies 2 Antibodies 2 Antibodies
Immunofluorescence (IF) 4 Antibodies 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 14 Antibodies 14 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies 8 Antibodies
Western Blotting (WB) 17 Antibodies 20 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 5 Antibodies
Dot Blot (DB) 11 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(93), (37), (34), (5), (4), (2), (1), (1)
Host Rabbit
(89), (4)
Clonality
Polyclonal
Conjugate This MAP3K8 antibody is un-conjugated
(5), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(49), (41), (32), (22), (13), (11), (7), (5), (3), (2)
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Product Details anti-MAP3K8 Antibody

Target Details MAP3K8 Application Details Handling Images
Specificity This antibody detects endogenous levels of MAP3K8 / TPL-2 protein(region surrounding Pro284).
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity Chromatography using epitope-specific immunogen

Target Details MAP3K8

Product Details anti-MAP3K8 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAP3K8 / TPL-2 (MAP3K8 Antibody Abstract)
Background The role of mitogen-activated protein kinases (MAPKs) in cell signaling pathways is well established. The rat gene Tpl-2, for tumor progression locus 2, and the human and mouse homologues c-Cot, for cancer osaka thyroid oncogene, encode a proto-oncogene serine/threonine protein kinase that was shown to play a role in the functional activation of the MAP kinase pathway. Overexpression of Cot induces MAP kinase activation in COS-1 and NIH/3T3 cells. Cot-mediated activation of MAP kinase is inhibited by both Ras N17, a dominant negative mutant of c-H-Ras, and Raf-1s621A, a dominant negative mutant of Raf-1, suggesting that Cot functions upstream of Ras and Raf-1.Other studies have shown that a kinase-negative, dominant negative mutant of Cot partially blocks Ras or Raf-1-induced MAP kinase activation, arguing that Cot functions downstream of Ras and Raf-1. To explain these contrasting findings, it has been suggested that Cot, Ras and Raf-1 may form a multimeric complex that phosphorylates MEK-1. Cot has also been shown to be implicated in T lymphocyte activation.Synonyms: COT, COT proto-oncogene serine/threonine-protein kinase, Cancer Osaka thyroid oncogene, ESTF, MAPK, Mitogen-activated protein kinase kinase kinase 8, Tumor progression locus 2, Tumor progression locus 2
Molecular Weight approx. 53 kDa
Gene ID 1326
NCBI Accession NP_005195
UniProt P41279
Pathways PI3K-Akt Signaling, TCR Signaling

Application Details

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Handling Images back to top
Application Notes ELISA: 1/40000approx. 1/60000. Western Blot: 1/500approx. 1/1000. Immunofluorescence: 1/50approx. 1/200. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details Handling back to top
Supplier Images
 image for anti-MAP3K8 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 8) (ABIN265369) anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) antibody
 image for anti-MAP3K8 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 8) (ABIN265369) anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) antibody (Image 2)