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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)||Human|
|ELISA||14 Antibodies||13 Antibodies||41 Antibodies|
|Immunochromatography (IC)||2 Antibodies||2 Antibodies||2 Antibodies|
|Immunofluorescence (IF)||4 Antibodies||7 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||14 Antibodies||14 Antibodies|
|Immunohistochemistry (IHC)||14 Antibodies||14 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies||8 Antibodies|
|Western Blotting (WB)||18 Antibodies||20 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies||5 Antibodies|
|Dot Blot (DB)||11 Antibodies|
|Enzyme Immunoassay (EIA)||3 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAP3K8 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-MAP3K8 AntibodyTarget Details MAP3K8 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of MAP3K8 / TPL-2 protein(region surrounding Pro284).|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||Affinity Chromatography using epitope-specific immunogen|
Target Details MAP3K8Product Details anti-MAP3K8 Antibody Application Details Handling Images back to top
|Alternative Name||MAP3K8 / TPL-2 (MAP3K8 Antibody Abstract)|
|Background||The role of mitogen-activated protein kinases (MAPKs) in cell signaling pathways is well established. The rat gene Tpl-2, for tumor progression locus 2, and the human and mouse homologues c-Cot, for cancer osaka thyroid oncogene, encode a proto-oncogene serine/threonine protein kinase that was shown to play a role in the functional activation of the MAP kinase pathway. Overexpression of Cot induces MAP kinase activation in COS-1 and NIH/3T3 cells. Cot-mediated activation of MAP kinase is inhibited by both Ras N17, a dominant negative mutant of c-H-Ras, and Raf-1s621A, a dominant negative mutant of Raf-1, suggesting that Cot functions upstream of Ras and Raf-1.Other studies have shown that a kinase-negative, dominant negative mutant of Cot partially blocks Ras or Raf-1-induced MAP kinase activation, arguing that Cot functions downstream of Ras and Raf-1. To explain these contrasting findings, it has been suggested that Cot, Ras and Raf-1 may form a multimeric complex that phosphorylates MEK-1. Cot has also been shown to be implicated in T lymphocyte activation.Synonyms: COT, COT proto-oncogene serine/threonine-protein kinase, Cancer Osaka thyroid oncogene, ESTF, MAPK, Mitogen-activated protein kinase kinase kinase 8, Tumor progression locus 2, Tumor progression locus 2|
|Molecular Weight||approx. 53 kDa|
|Pathways||PI3K-Akt Signaling, TCR Signaling|
Application DetailsProduct Details anti-MAP3K8 Antibody Target Details MAP3K8 Handling Images back to top
ELISA: 1/40000approx. 1/60000. Western Blot: 1/500approx. 1/1000. Immunofluorescence: 1/50approx. 1/200. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details Handling back to top