|Application / Reactivity||Human||Mouse (Murine)||Rabbit||Rat (Rattus)||Cow (Bovine)|
|Immunofluorescence (fixed cells) (IF/ICC)||5 Antibodies||2 Antibodies||1 Antibodies||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||148 Antibodies||64 Antibodies||45 Antibodies||14 Antibodies||10 Antibodies|
|Immunoprecipitation (IP)||9 Antibodies||4 Antibodies||5 Antibodies||4 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||38 Antibodies||16 Antibodies||18 Antibodies||4 Antibodies||3 Antibodies|
|Antigen||Neutrophil Cytosol Factor 1 (NCF1) Antibodies|
|Reactivity||Cow (Bovine), Human, Mouse (Murine), Rabbit, Rat (Rattus) Alternatives|
|Conjugate||This NCF1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-NCF1 AntibodyTarget Details NCF1 Application Details Handling Images
|Specificity||Recognizes Neutrophil Cytosol Factor 1 (NCF1).|
Species reactivity (expected):Bovine, Mouse, Rabbit and Rat.
Species reactivity (tested):Human.
|Immunogen||Synthetic peptide -KLH conjugated corresponding to C Terminus of NCF1.|
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|Alternative Name||NCF1 (NCF1 Antibody Abstract)|
|Background||NCF1, along with NCF2 and a membrane bound cytochrome b558, is required for activation of the latent NADPH oxidase necessary for superoxide production. Defects in NCF1 are the cause of autosomal cytochrome-b-positive chronic granulomatous disease type 1 (CGD). NCF1 is a cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils.Synonyms: 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-1, NCF-47K, NOXO2, Neutrophil NADPH oxidase factor 1, Neutrophil cytosol factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, SH3PXD1A, p47-phox|
|Research Area||Cancer, Metabolism|
Application DetailsProduct Details anti-NCF1 Antibody Target Details NCF1 Handling Images back to top
ELISA. Western Blot: 0.2-2.0 μg/mL. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
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|Buffer||TRIS Buffered Saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
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